Role of microRNA-7 in digestive system malignancy

Chen, Wanqun

doi:10.4251/wjgo.v8.i1.121

Cited by 19 publications

(18 citation statements)

References 52 publications

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“…miRNA- (miR-) 7 is an evolutionarily conserved miRNA abundantly expressed in the human pancreas and endocrine cells, which plays specific roles in the endocrine cell differentiation and function [ 10 ]. Moreover, it has been shown that miR-7 is associated with the progression of various tumors, including gastric cancer, lung cancer, breast cancer, and glioma [ 11 ]. DNA methylation-mediated miR-7-5p silencing would promote the gastric cancer stem cell invasion by increasing Smo and Hes1 [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Nei Endonuclease VIII-Like1 (NEIL1) Inhibits Apoptosis of Human Colorectal Cancer Cells

Xue

Liu

Xin

et al. 2020

BioMed Research International

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The study is aimed at investigating the role of Nei endonuclease VIII-like1 (NEIL1) in the pathogenesis of colorectal cancer (CRC). The human CRC (HCT116 and SW480) cells were subjected to the siRNA silencing and recombinant plasmid overexpression of NEIL1. Transfection of siNEIL1 significantly inhibited the cell growth. It also increased the Bax expression levels, while it decreased the Bcl-2 expression levels in human CRC cells, leading the Bax/Bcl-2 balance toward apoptosis. Moreover, the apoptosis was promoted through the caspase-9 signaling pathway. One the other hand, high expression of NEIL1 promoted the cell viability and reduced the apoptosis, inducing the balance of Bax/Bcl-2 in the human colon cancer cells to be antiapoptotic. In addition, the caspase-9 signaling pathway inhibited apoptosis, contrary to the results obtained by downregulating NEIL1 expression. Furthermore, NEIL1 was negatively regulated by miR-7-5p, indicating that miR-7-5p inhibited the NEIL1 expression after transcription. Overexpression of miR-7-5p reversed the effects of NEIL1 on these CRC cells. In conclusion, NEIL1 promotes the proliferation of CRC cells, which is regulated negatively by miR-7-5p. These findings suggest that NEIL1 is a potential therapeutic target for CRC.

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Section: Introductionmentioning

confidence: 99%

Nei Endonuclease VIII-Like1 (NEIL1) Inhibits Apoptosis of Human Colorectal Cancer Cells

Xue

Liu

Xin

et al. 2020

BioMed Research International

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“…A variety of miRNAs have been reported to serve as anti-oncogenes in CRC, including miR-483 and miR-551 ( 8 ). miR-7 is an evolutionarily conserved miRNA that is involved in the development of the eye and pancreas in Drosophila ( 9 ). Accumulating evidence suggests that miR-7 simultaneously targets a number of mRNAs that are involved in various signaling pathways in several types of cancer ( 9 ).…”

Section: Introductionmentioning

confidence: 99%

“…miR-7 is an evolutionarily conserved miRNA that is involved in the development of the eye and pancreas in Drosophila ( 9 ). Accumulating evidence suggests that miR-7 simultaneously targets a number of mRNAs that are involved in various signaling pathways in several types of cancer ( 9 ). It has been demonstrated that miR-7 is downregulated in certain human tumors, including CRC, and this miRNA has been reported to regulate a number of oncogenic signal transduction pathways, including the epidermal growth factor receptor signaling pathway, as well as the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and RAF/mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (RAF/MEK/ERK) pathways, suggesting that it may function as a tumor suppressor ( 10 , 11 ).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-7 inhibits colorectal cancer cell proliferation, migration and invasion via TYRO3 and phosphoinositide 3-kinase/protein B kinase/mammalian target of rapamycin pathway suppression

Qin

Qian

2018

Int J Mol Med

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MicroRNA-7 (miR-7) is a non-coding RNA that inhibits colorectal cancer (CRC) cell proliferation, migration and invasion. miR-7 effectively silences TYRO3 expression, and the close association between TYRO3 and CRC has previously been reported. Therefore, the present study aimed to assess the roles and molecular mechanisms of TYRO3 and miR-7 in the development of CRC. The expression levels of miR-7 and TYRO3 in CRC tissues and cell lines were detected by reverse transcription-quantitative polymerase chain reaction. A dual-luciferase reporter assay was also performed to confirm whether TYRO3 was a target of miR-7. The effect of miR-7 and TYRO3 on cell proliferation, migration and invasion was detected in vitro with MTT, wound healing and cell invasion assays, respectively. The expression of proteins associated with the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway was detected by western blot analysis. The results indicated that the expression of miR-7 was downregulated and the levels of TYRO3 were gradually increased in CRC tissues and cell lines. TYRO3 was identified as a functional target of miR-7, and its expression was negatively regulated by miR-7 in CRC cell lines. The functional studies demonstrated that miR-7 knockdown promoted the proliferation, migration and invasion of CRC cells, while knockdown of TYRO3 repressed these biological processes. In addition, pathway analyses revealed that the oncogenic effect of TYRO3 was associated with PI3K/AKT/mTOR pathway inhibition. In conclusion, the data suggested that miR-7 promoted the development of CRC by targeting oncogenic TYRO3, which may be mediated by inhibition of the PI3K/AKT/mTOR signaling pathway. Thus, miR-7 may serve as an independent prognostic biomarker in patients with CRC.

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“…The digestive system is composed of many ducts and glands, and because of its complicated physiology and anatomy, numerous diseases are prone to occur, especially malignancies, which have become one of the most terrible threats for human health [ 1 ]. According to the 2015 cancer statistics, the cancer-related mortality has been continuously declining for the past two decades.…”

Section: Introductionmentioning

confidence: 99%

Poor prognosis of hexokinase 2 overexpression in solid tumors of digestive system: a meta-analysis

Zhou

et al. 2017

Oncotarget

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Several previous studies have reported the prognostic value of hexokinase 2 (HK2) in digestive system tumors. However, these studies were limited by the small sample sizes and the results were inconsistent among them. Therefore, we conducted a meta-analysis based on 15 studies with 1932 patients to assess the relationship between HK2 overexpression and overall survival (OS) of digestive system malignancies. The relationship of HK2 and clinicopathological features was also evaluated. Hazard ratio (HR) or odds ratio (OR) with its 95% confidence intervals (CI) were calculated to estimate the effect size. Positive HK2 expression showed poor OS in all tumor types (HR = 1.75 [1.41-2.18], P < 0.001). When stratified by tumor type, the impact of HK2 overexpression on poor prognosis was observed in gastric cancer (HR = 1.77 [1.25-2.50], P < 0.001), hepatocellular carcinoma (HR = 1.87 [1.58-2.21], P < 0.001), and colorectal cancer (HR = 2.89 [1.62-5.15], P < 0.001), but not in pancreatic ductal adencarcinoma (HR = 1.11 [0.58-2.11], P = 0.763). Furthermore, high HK2 expression was significantly associated with some phenotypes of tumor aggressiveness, such as large tumor size (OR = 2.03 [1.10-3.74], P = 0.024), positive lymph node metastasis (OR = 2.05 [1.39-3.02], P < 0.001), advanced clinical stage (OR = 2.17 [1.21-3.89], P = 0.009) and high alpha fetoprotein level (OR = 1.47 [1.09-2.02] P = 0.013). In summary, HK2 might act as a prognostic indicator and a potential therapeutic target of these digestive system cancers.

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Role of microRNA-7 in digestive system malignancy

Cited by 19 publications

References 52 publications

Nei Endonuclease VIII-Like1 (NEIL1) Inhibits Apoptosis of Human Colorectal Cancer Cells

Nei Endonuclease VIII-Like1 (NEIL1) Inhibits Apoptosis of Human Colorectal Cancer Cells

MicroRNA-7 inhibits colorectal cancer cell proliferation, migration and invasion via TYRO3 and phosphoinositide 3-kinase/protein B kinase/mammalian target of rapamycin pathway suppression

Poor prognosis of hexokinase 2 overexpression in solid tumors of digestive system: a meta-analysis

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