Role of MicroRNA-31 (miR-31) in Breast Carcinoma Diagnosis and Prognosis

Gharib, Amal F.; Khalifa, Amany Salah; Eed, Emad M.; Banjer, Hamsa Jameel; Shami, Ashjan; Askary, Ahmad El; Elsawy, Wael H.

doi:10.21873/invivo.12857

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…Breast cancer is the most common malignancy in women, with the occurrence, development, and metastasis of cancer cells being the primary cause of death. Breast cancer is a multifactorial, multistage process where normal mammary cells undergo epigenetic changes to become malignant, forming cancerous tumor cells which then proliferate to form malignant tumors [12,13]. Although many oncogenes and tumor To further study the molecular mechanism by which miR-20b promotes breast cancer cell proliferation, we confirmed through a luciferase reporter assay that miR-20b can directly target the 3'-UTR of HIF-α mRNA, thereby reducing the expression levels of HIF-α mRNA and protein.…”

Section: Discussionmentioning

confidence: 99%

The effect of miR-20b on breast cancer cell proliferation and related mechanism

Li,

He,

Cui

et al. 2024

Preprint

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Background and Purpose Breast cancer is the the most common malignant tumor of women, and contributes to the main cause of death of female malignant tumors. Although there are many mutated oncogenes and anti-oncogenes during the course of breast cancer formation, it is not clear that the specific molecular mechanism to maintain the malignant proliferation of tumor cells. Studies have shown that microRNAs (miRNAs) display specific expression in different tumor tissues and play an important role in the development of tumors. The purpose of this paper is to explore the influences of miR-20b on the breast cancer cell proliferation and the regulation function of miR-20b to the targeted genes.It will provide evidence for diagnosis and treatment of breast cancer and lay a foundation for its clinical targeted diagnosis and treatment. Methods We analyzed the expressive levels of miR-20b within cancer tissue and corresponding normal tissue adjacent to carcinoma of 10 breast cancer patients, as well as the normal breast epithelial cell lines MCF-10A and SKBR3, MCF-7, and MDA-MB-231 breast cancer cell lines through quantitative PCR (qPCR). MDA-MB-231 breast cancer cell line was up-regulated by miR-20b, and the effect of miR-20b on proliferation ability of breast cancer cells were detected by MTT and colony formation assay. The effect of miR-20b on the tumor formation ability of 231 breast cancer cells was detected by the breast cancer model of BALB/c mice. we used the bioinformatics to predict the possible downstream target genes, and verified it through luciferase experiment. Finally, the regulation effect of miR-20b on target genes was detected by qPCR and western blot in mRNA and protein levels. Results The present study confirmed that the expressive level of miR-20b were significantly reduced in breast cancer tissue and cells. MTT and colony formation data showed that miR-20b inhibited the proliferation ability of 231 breast cancer cells, and the breast cancer model of BALB/c nude mice showed that miR-20b inhibited the tumor formation ability of 231 breast cancer cells. The luciferase experiment confirmed that hypoxia inducible factor-α(HIF-α)was a potential downstream target gene of miR-20b. Finally we found by qPCR and western blot that miR-20b could inhibit the expression of target gene HIF-α in mRNA and protein levels. Conclusions MiR-20b can inhibit the proliferation of breast cancer cells. MiR-20b can inhibit the expression of target gene HIF-α in mRNA and protein levels.

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Section: Discussionmentioning

confidence: 99%

The effect of miR-20b on breast cancer cell proliferation and related mechanism

Li,

He,

Cui

et al. 2024

Preprint

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“…Non-circulating oncomiR/ Suppressor -Correlated with poor prognosis [208] miR-2117 Non-circulating oncomiR -Poor survival -Large tumor size [116] miR-519c-3p Non-circulating oncomiR -Associated with a large tumor size [116] miR-873-5p Non-circulating Suppressor -Promotes tumor development and metastasis [209] miR-133 Non-circulating oncomiR -Induces proliferation and colony formation [198] miR-585 Non-circulating oncomiR -Promotes cell proliferation, migration, and invasion -Significantly associated with poor prognosis [210] Indeed, both tissue and circulating miRNAs are deregulated in TNBCs and are implicated with the various pathophysiological processes of initiation, development, and the progression of tumors, which may have the potential to help in the discovery of new diagnostic, prognostic, and therapeutic strategies.…”

Section: Mirna Implications In Breast Cancermentioning

confidence: 99%

ER Negative Breast Cancer and miRNA: There Is More to Decipher Than What the Pathologist Can See!

Chamandi,

El-Hajjar,

El Kurdi

et al. 2023

Biomedicines

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Breast cancer (BC), the most prevalent cancer in women, is a heterogenous disease. Despite advancements in BC diagnosis, prognosis, and therapeutics, survival rates have drastically decreased in the metastatic setting. Therefore, BC still remains a medical challenge. The evolution of high-throughput technology has highlighted gaps in the classification system of BCs. Of particular interest is the notorious triple negative BC, which was recounted as being heterogenous itself and it overlaps with distinct subtypes, namely molecular apocrine (MA) and luminal androgen (LAR) BCs. These subtypes are, even today, still misdiagnosed and poorly treated. As such, researchers and clinicians have been looking for ways through which to refine BC classification in order to properly understand the initiation, development, progression, and the responses to the treatment of BCs. One tool is biomarkers and, specifically, microRNA (miRNA), which are highly reported as associated with BC carcinogenesis. In this review, the diverse roles of miRNA in estrogen receptor negative (ER−) and androgen receptor positive (AR+) BC are depicted. While highlighting their oncogenic and tumor suppressor functions in tumor progression, we will discuss their diagnostic, prognostic, and predictive biomarker potentials, as well as their drug sensitivity/resistance activity. The association of several miRNAs in the KEGG-reported pathways that are related to ER-BC carcinogenesis is presented. The identification and verification of accurate miRNA panels is a cornerstone for tackling BC classification setbacks, as is also the deciphering of the carcinogenesis regulators of ER − AR + BC.

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Latest advances in the study of non-coding RNA-mediated circadian rhythm disorders causing endometrial cancer

Zheng,

Chen,

Zhou

et al. 2023

Front. Oncol.

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Endometrial cancer (EC) is one of the most common gynecological cancers, and its risk factors include obesity and metabolic, genetic, and other factors. Recently, the circadian rhythm has also been shown to be associated with EC, as the severity of EC was found to be related to night work and rhythm disorders. Therefore, circadian rhythm disorders (CRDs) may be one of the metabolic diseases underlying EC. Changes in the circadian rhythm are regulated by clock genes (CGs), which in turn are regulated by non-coding RNAs (ncRNAs). More importantly, the mechanism of EC caused by ncRNA-mediated CRDs is gradually being unraveled. Here, we review existing studies and reports and explore the relationship between EC, CRDs, and ncRNAs.

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Role of MicroRNA-31 (miR-31) in Breast Carcinoma Diagnosis and Prognosis

Cited by 4 publications

References 33 publications

The effect of miR-20b on breast cancer cell proliferation and related mechanism

The effect of miR-20b on breast cancer cell proliferation and related mechanism

ER Negative Breast Cancer and miRNA: There Is More to Decipher Than What the Pathologist Can See!

Latest advances in the study of non-coding RNA-mediated circadian rhythm disorders causing endometrial cancer

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