Role of microRNA 146a on the healing of cornea alkali burn treated with mesenchymal stem cells

Luo, Xu; Li, Jianmin; Liang, Yin; Pan, Jian; Zhang, Yang; Jiang, Zipei

doi:10.3892/mmr.2018.9328

Cited by 8 publications

(13 citation statements)

References 26 publications

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“…MiRNAs, a subclass of 18–24 bp long non-coding single-stranded RNA, are an important regulatory RNA component of EVs. MiRNAs regulate stability as well as post-transcriptional regulation of gene expression by binding to target messenger RNAs (mRNAs) [ 54 ]. These biomolecules participate in various diseases including chronic inflammatory diseases, cancer, heart diseases, and ophthalmic diseases.…”

Section: Limiting Damage After Corneal Burn Injurymentioning

confidence: 99%

“…In the case of corneal injury, the role of miRNAs on phenomena such as wound repair, inflammation, maintenance of the corneal/ limbal stem cell pool, lymphangiogenesis, and corneal neovascularization has been studied in the pre-clinical setting. For instance, in a rat model of alkali-induced burn injury, the authors evaluated the therapeutic effect of bone marrow-derived MSCs engineered to over-express miR146a on corneal repair [ 54 ]. A reduction in corneal opacification was observed in vivo with improvement in angiogenesis through the inhibition of VEGF, CD45 and Interferon-γ [ 54 ].…”

Section: Limiting Damage After Corneal Burn Injurymentioning

confidence: 99%

“…For instance, in a rat model of alkali-induced burn injury, the authors evaluated the therapeutic effect of bone marrow-derived MSCs engineered to over-express miR146a on corneal repair [ 54 ]. A reduction in corneal opacification was observed in vivo with improvement in angiogenesis through the inhibition of VEGF, CD45 and Interferon-γ [ 54 ]. RNA-seq and qualitative proteomics analyses revealed that miR146a was upregulated in the limbal region rich in stem cells.…”

Section: Limiting Damage After Corneal Burn Injurymentioning

confidence: 99%

“…In summary, EVs represent an innovative and very promising drug delivery system, and EV-based therapeutics offer new hopes to satisfy unmet clinical needs. [54]. These biomolecules participate in various diseases including chronic inflammatory diseases, cancer, heart diseases, and ophthalmic diseases.…”

Section: Cell-free Strategies: Ev-based Therapeutic Approaches For Corneal Burn Injurymentioning

confidence: 99%

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Regenerative Approaches and Future Trends for the Treatment of Corneal Burn Injuries

Saccu

Menchise

Giordano³

et al. 2021

JCM

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Ocular chemical and thermal burns are frequent causes of hospitalization and require immediate interventions and care. Various surgical and pharmacological treatment strategies are employed according to damage severity. Controlling inflammation and neovascularization while promoting normal ocular surface anatomy and function restoration is the principal aim. In the most severe cases, when epithelial healing is severely affected, reconstruction of the ocular surface may be a valid option, which, however, requires expertise, adequate instruments, and qualified donors. Numerous endogenous and exogenous strategies have been considered for corneal repair. Among these, stem cells and their derivatives have offered numerous attractive possibilities in finding an effective way in stimulating corneal regeneration. Limbal epithelial stem cells and mesenchymal cells from the ocular tissue as well as from various sources have demonstrated their effectiveness in dampening neovascularization, scarring, and inflammation, while promoting epithelialization of the injured cornea. Moreover, a plethora of cytokines and growth factors, and extracellular vesicles, which constitute the secretome of these cells, work in concert to enhance wound healing. In this review, we provide an update on the recent potential therapeutic avenues and clinical applications of stem cells and their products in corneal regeneration after burn injury, as well as current imaging strategies for monitoring therapeutic efficacy and damage resolution.

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Section: Limiting Damage After Corneal Burn Injurymentioning

confidence: 99%

Section: Limiting Damage After Corneal Burn Injurymentioning

confidence: 99%

Section: Limiting Damage After Corneal Burn Injurymentioning

confidence: 99%

Section: Cell-free Strategies: Ev-based Therapeutic Approaches For Corneal Burn Injurymentioning

confidence: 99%

See 2 more Smart Citations

Regenerative Approaches and Future Trends for the Treatment of Corneal Burn Injuries

Saccu

Menchise

Giordano³

et al. 2021

JCM

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show abstract

“…From this, we can infer that these cells have potential to control the microenvironment during local inflammation, and are candidates for allogeneic cell-based therapies. There have been several studies investigating the use of MSCs from other tissue (bone marrow or adipose tissue) in treating corneal disease to differing success[16-19]. The use of MSCs from tissues other than the cornea has shown limitations for corneal disease models.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model

Morales¹,

Marsit²,

McIntosh³

et al. 2019

WJSC

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BACKGROUND An in vitro injury model mimicking a corneal surface injury was optimised using human corneal epithelial cells (hCEC). AIM To investigate whether corneal-stroma derived stem cells (CSSC) seeded on an amniotic membrane (AM) construct manifests an anti-inflammatory, healing response. METHODS Treatment of hCEC with ethanol and pro-inflammatory cytokines were compared in terms of viability loss, cytotoxicity, and pro-inflammatory cytokine release, in order to generate the in vitro injury. This resulted in an optimal injury of 20% (v/v) ethanol for 30 s with 1 ng/mL interleukin-1 (IL-1) beta. Co-culture experiments were performed with CSSC alone and with CSSC-AM constructs. The effect of injury and co-culture on viability, cytotoxicity, IL-6 and IL-8 production, and IL1B , TNF , IL6 , and CXCL8 mRNA expression were assessed. RESULTS Co-culture with CSSC inhibited loss of hCEC viability caused by injury. Enzyme linked immunosorbent assay and polymerase chain reaction showed a significant reduction in the production of IL-6 and IL-8 pro-inflammatory cytokines, and reduction in pro-inflammatory cytokine mRNA expression during co-culture with CSSC alone and with the AM construct. These results confirmed the therapeutic potential of the CSSC and the possible use of AM as a cell carrier for application to the ocular surface. CONCLUSION CSSC were shown to have a potentially therapeutic anti-inflammatory effect when treating injured hCEC, demonstrating an important role in corneal regeneration and wound healing, leading to an improved knowledge of their potential use for research and therapeutic purposes.

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Surgical Management and Recent Advances in Chemical Injury: A 5-year Review

Awasthi

Singh

Raj

2021

Seminars in Ophthalmology

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Role of microRNA 146a on the healing of cornea alkali burn treated with mesenchymal stem cells

Cited by 8 publications

References 26 publications

Regenerative Approaches and Future Trends for the Treatment of Corneal Burn Injuries

Regenerative Approaches and Future Trends for the Treatment of Corneal Burn Injuries

Anti-inflammatory potential of human corneal stroma-derived stem cells determined by a novel in vitro corneal epithelial injury model

Surgical Management and Recent Advances in Chemical Injury: A 5-year Review

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