Role of Microorganisms in Lignocellulosic Biodegradation

Rana, Vandana; Rana, Diwakar

doi:10.1007/978-3-319-47379-6_2

Cited by 2 publications

(2 citation statements)

References 291 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cellulases generally include three classes of glycoside hydrolases: cellobiohydrolase (EC 3.2.1.91), endo-1,4-β-glucanase (EC 3.2.1.4), and β-glucosidase (EC 3.2.1.21). The action of these enzymes generates products such as oligosaccharides, cellobiose, and glucose [ 11 , 13 , 14 , 15 ]. Xylan is the main component of hemicellulose, composed of a linear backbone of β-D-1,4-xylopyranose units, and it is hydrolyzed by two enzymes, endo xylanase (EC 3.2.1.8) and β-xylosidase (EC 3.2.1.37).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TtCel7A: A Native Thermophilic Bifunctional Cellulose/Xylanase Exogluclanase from the Thermophilic Biomass-Degrading Fungus Thielavia terrestris Co3Bag1, and Its Application in Enzymatic Hydrolysis of Agroindustrial Derivatives

López-López

Santiago-Hernández

Cayetano-Cruz

et al. 2023

JoF

View full text Add to dashboard Cite

The biomass-degrading thermophilic ascomycete fungus Thielavia terrestris Co3Bag1 produces TtCel7A, a native bifunctional cellulase/xylanase GH7 family. The purified TtCel7A, with an estimated molecular weight of 71 kDa, was biochemically characterized. TtCel7A displayed an optimal pH of 5.5 for both activities and an optimal temperature of 60 and 50 °C for cellulolytic and xylanolytic activities, respectively. The half-lives determined for cellulase activity were 140, 106, and 41 min at 50, 60, and 70 °C, respectively, whereas the half-lives observed for xylanase activity were 24, 10, and 1.4 h at 50, 60, and 70 °C, respectively. The KM and Vmax values were 3.12 mg/mL and 50 U/mg for cellulase activity and 0.17 mg/mL and 42.75 U/mg for xylanase activity. Circular dichroism analysis suggests changes in the secondary structure of TtCel7A in the presence of CMC as the substrate, whereas no modifications were observed with beechwood xylan. TtCel7A displayed the excellent capability to hydrolyze CMC, beechwood xylan, and complex substrates such as oat bran, wheat bran, and sugarcane bagasse, with glucose and cellobiose being the main products released; also, slightly less endo cellulase and xylanase activities were observed. Thus, suggesting TtCel7A has an exo- and endomode of action. Based on the characteristics of the enzyme, it might be considered a good candidate for industrial applications.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Xylan is the main component of hemicellulose, composed of a linear backbone of β-D-1,4-xylopyranose units, and it is hydrolyzed by two enzymes, endo xylanase (EC 3.2.1.8) and β-xylosidase (EC 3.2.1.37). The action of xylanases generates xylooligosaccharides and monosaccharides [ 3 , 7 , 14 , 16 ].…”

Section: Introductionmentioning

confidence: 99%