Role of metabolites in flower development and discovery of compounds controlling flowering time

Chakraborty, Anwesha; Chaudhury, Rim; Dutta, Shyamali; Basak, Mridushree; Dey, Sonali; Schäffner, Anton R.; Das, Mita

doi:10.1016/j.plaphy.2022.09.002

Cited by 12 publications

(12 citation statements)

References 105 publications

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“…Here, the effect of the CHS gene, which is responsible for synthesizing phenolic compounds (13) from the phenylpropanoid pathways, is related to the flowering process. It is known that the CHS gene is involved in the synthesis of phenolics known to have a flowering suppressant effect (32). According to our findings, CHS gene expression increased UV‐B application, while it decreased in silicon groups (Fig.…”

Section: Resultsmentioning

confidence: 99%

Molecular Effects of Silicon on Arabidopsis thaliana Seedlings under UV‐B Stress

Celayir

Yeni

Yeşildirek

et al. 2023

Photochem & Photobiology

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Silicon‐plant interaction studies have shown that silicon reduces the harmful effects of stress in plants. Ultraviolet‐B (UV‐B) radiation, one of the abiotic stress affecting plants, poses a severe problem due to global warming. In this context, it is crucial to examine silicon's effects on UV‐B radiation stress at the molecular level. The experiments were carried out on 17 days old Arabidopsis seedlings that were treated with 800 μWatt cm−2 doses of UV‐B for 60 min and harvested on the 28th day. 1 mM orthosilicic acid was applied to the in vitro plant tissue culture for experimental groups. According to the results of the osmolyte accumulation analyses, silicon has been shown to play a role in the osmotic stress response. Gene expression levels of DGK2, CHS, FLC, RAD51, and UVR8 were measured via qPCR, and it has been shown that silicon interacts with these genes under UV‐B radiation stress. The result of genomic DNA methylation analysis demonstrated that silicon might affect DNA methylation levels by increasing the 5‐mC percentage compared with the control group. This study focused on the molecular effects of silicon application. It supports silicon–plant interaction research by demonstrating that silicon might affect UV‐B response at the molecular level.

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Section: Resultsmentioning

confidence: 99%

Molecular Effects of Silicon on Arabidopsis thaliana Seedlings under UV‐B Stress

Celayir

Yeni

Yeşildirek

et al. 2023

Photochem & Photobiology

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“…In combination with our metabolomic studies, flavonoids play an important role in the secondary flowering process ( Figures 5 , 6 ). Summer heat stress affects lipid content ( Lahlali et al., 2014 ) as a signalling metabolite for flowering time in Arabidopsis and oilseed rape, which can lead to earlier flowering through up-regulation of FT genes ( Chakraborty et al., 2022 ). Our study also shows that lipids can be involved in other pathways to co-regulate secondary flowering in chestnut.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to hormones, lipids can be used as signalling metabolites for flowering time in Arabidopsis and oilseed rape. Phenolics and phenylpropanoids are also involved in the regulation of plant flowering ( Chakraborty et al., 2022 ). The expression of genes related to the anthocyanin synthesis pathway, such as Chalcone synthase (CHS) , Chalcone isomerase (CHI) , and Dihydroflavonol-4-reductase (DFR) , under a light/dark cycle exhibits a circadian rhythm ( Nguyen and Lee, 2016 ; Hildreth et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated transcriptome, metabolome and phytohormone analysis reveals developmental differences between the first and secondary flowering in Castanea mollissima

Cai

Zhang

et al. 2023

Front. Plant Sci.

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IntroductionChestnut (Castanea mollissima BL.) is an important woody grain, and its flower formation has a significant impact on fruit yield and quality. Some chestnut species in northern China re-flower in the late summer. On the one hand, the second flowering consumes a lot of nutrients in the tree, weakening the tree and thus affecting flowering in the following year. On the other hand, the number of female flowers on a single bearing branch during the second flowering is significantly higher than that of the first flowering, which can bear fruit in bunches. Therefore, these can be used to study the sex differentiation of chestnut.MethodsIn this study, the transcriptomes, metabolomes, and phytohormones of male and female chestnut flowers were determined during spring and late summer. We aimed to understand the developmental differences between the first and secondary flowering stages in chestnuts. We analysed the reasons why the number of female flowers is higher in the secondary flowering than in the first flowering and found ways to increase the number of female flowers or decrease the number of male flowers in chestnuts.ResultsTranscriptome analysis of male and female flowers in different developmental seasons revealed that EREBP-like mainly affected the development of secondary female flowers and HSP20 mainly affected the development of secondary male flowers. KEGG enrichment analysis showed that 147 common differentially-regulated genes were mainly enriched from circadian rhythm-plant, carotenoid biosynthesis, phenylpropanoid biosynthesis, and plant hormone signal transduction pathways. Metabolome analysis showed that the main differentially accumulated metabolites in female flowers were flavonoids and phenolic acids, whereas the main differentially accumulated metabolites in male flowers were lipids, flavonoids, and phenolic acids. These genes and their metabolites are positively correlated with secondary flower formation. Phytohormone analysis showed that abscisic and salicylic acids were negatively correlated with secondary flower formation. MYB305, a candidate gene for sex differentiation in chestnuts, promoted the synthesis of flavonoid substances and thus increased the number of female flowers.DiscussionWe constructed a regulatory network for secondary flower development in chestnuts, which provides a theoretical basis for the reproductive development mechanism of chestnuts. This study has important practical implications for improving chestnut yield and quality.

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“…Agmatine deiminase and spermidine synthase were more abundant ( Figure 3 a), and, in agreement, polyamines were also more abundant, including spermidine (SA t1 ), cadaverine (SA t1 , SA t2 ), and putrescine (SA t1 , Table S3 , p < 0.1). There is evidence that polyamines protect reproductive tissues during stress exposure and that external application of polyamines could accelerate flowering [ 7 ]. The accumulation of polyamines was not specific to SA t2 seeds.…”

Section: Discussionmentioning

confidence: 99%

“…These factors integrate internal signals and external stimuli, and it is a period highly sensitive to stress [ 6 ]. Plants’ response to stressors may vary depending on the species and severity of the stress, but in general, flowering time can either be accelerated or delayed [ 7 ]. Salicylic acid is one of the transmissible signals that are produced to induce flowering under stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Salicylic Acid Treatment and Its Effect on Seed Yield and Seed Molecular Composition of Pisum sativum under Abiotic Stress

Berková

Berka

Kameniarová

et al. 2023

IJMS

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The reproductive stage of plant development has the most critical impact on yield. Flowering is highly sensitive to abiotic stress, and increasing temperatures and drought harm crop yields. Salicylic acid is a phytohormone that regulates flowering and promotes stress resilience in plants. However, the exact molecular mechanisms and the level of protection are far from understood and seem to be species-specific. Here, the effect of salicylic acid was tested in a field experiment with Pisum sativum exposed to heat stress. Salicylic acid was administered at two different stages of flowering, and its effect on the yield and composition of the harvested seeds was followed. Plants treated with salicylic acid produced larger seed pods, and a significant increase in dry weight was found for the plants with a delayed application of salicylic acid. The analyses of the seed proteome, lipidome, and metabolome did not show any negative impact of salicylic treatment on seed composition. Identified processes that could be responsible for the observed improvement in seed yields included an increase in polyamine biosynthesis, accumulation of storage lipids and lysophosphatidylcholines, a higher abundance of components of chromatin regulation, calmodulin-like protein, and threonine synthase, and indicated a decrease in sensitivity to abscisic acid signaling.

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Role of metabolites in flower development and discovery of compounds controlling flowering time

Cited by 12 publications

References 105 publications

Molecular Effects of Silicon on Arabidopsis thaliana Seedlings under UV‐B Stress

Molecular Effects of Silicon on Arabidopsis thaliana Seedlings under UV‐B Stress

Integrated transcriptome, metabolome and phytohormone analysis reveals developmental differences between the first and secondary flowering in Castanea mollissima

Salicylic Acid Treatment and Its Effect on Seed Yield and Seed Molecular Composition of Pisum sativum under Abiotic Stress

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