Role of Meta-topolin on in Vitro Shoot Regeneration: An Insight

Gantait, Saikat; Mitra, Monisha

doi:10.1007/978-981-15-9046-7_12

Cited by 10 publications

(4 citation statements)

References 91 publications

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“…m T at 4 μM gave the maximum results (3.28) recorded for shoot number. These findings confirmed that the optimal concentration of m T for shoot multiplication of apple depended not only on the examined genotype but also on the type of explant used, as reported by Gantait and Mitra [ 52 ]. Other aspects of m T, apart from effective induction and multiplication of shoots, are the increase in shoot length, fresh weight, and photosynthetic capacity of in vitro-derived plants [ 44 , 66 ].…”

Section: Discussionsupporting

confidence: 88%

“…Shoot proliferation and multiplication during micropropagation process is affected by the type and level of PGRs applied in culture media; CKs especially have governing effects due to their significant functions in cell division, organogenesis, and suppressing the apical dominance, thus enhancing shoot formation from lateral buds [ 50 , 51 , 52 ]. CKs are widely added in shoot cultures to regulate the in vitro growth and development of newly regenerated shoots [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…It could prevent shoot-tip necrosis, and help evade the effects of hyperhydricity. It has tremendous potential in in vitro shoot regeneration by delaying senescence, which ultimately facilitates multiple shoot induction, proliferation, and regeneration, and increases shoot length [ 52 , 60 ]. m T has proven to be an effective cytokinin for inducing multiple shoot proliferation in a number of plant species via direct and indirect organogenesis [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

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Meta-Topolin as an Effective Benzyladenine Derivative to Improve the Multiplication Rate and Quality of In Vitro Axillary Shoots of Húsvéti Rozmaring Apple Scion

Abdalla,

Dobránszki

2024

Plants

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In vitro mass propagation of apple plants plays an important role in the rapid multiplication of genetically uniform, disease-free scions and rootstocks with desired traits. Successful micropropagation of apple using axillary shoot cultures is influenced by several factors, the most critical of which is the cytokinin included in the culture medium. The impact of medium composition from single added cytokinins on shoot proliferation of apple scion Húsvéti rozmaring cultured on agar-agar gelled Murashige and Skoog medium fortified with indole butyric acid and gibberellic acid was investigated. The optimum concentration for efficient shoot multiplication differs according to the type of cytokinin. The highest significant multiplication rate (5.40 shoots/explant) was achieved using 2.0 μM thidiazuron while the longest shoots (1.80 cm) were observed on the medium containing benzyladenine at a concentration of 2.0 μM. However, application of either thidiazuron or benzyladenine as cytokinin source in the medium resulted in shoots of low quality, such as stunted and thickened shoots with small leaves. In the case of benzyladenine riboside, the 8 μM concentration was the most effective in increasing the multiplication rate (4.76 shoots/explant) but caused thickened stem development with tiny leaves. In the present study, meta-topolin was shown to be the most effective cytokinin that could be applied to induce sufficient multiplication (3.28 shoots/explant) and high-quality shoots along with shoot lengths of 1.46 cm when it was applied at concentrations of 4 μM. However, kinetin was the least active cytokinin; it practically did not induce the development of new shoots. The superior cytokinin for in vitro axillary shoot development of apple scion Húsvéti rozmaring with high-quality shoots was the meta-topolin, but it may be different depending on the variety/genotype under study.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Meta-Topolin as an Effective Benzyladenine Derivative to Improve the Multiplication Rate and Quality of In Vitro Axillary Shoots of Húsvéti Rozmaring Apple Scion

Abdalla,

Dobránszki

2024

Plants

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“…As mentioned above, in the last few years, m-T has been proven as an alternative to conventional CK for in vitro propagation of plants [55]. In this sense, the advantages of m-T to promote rhizogenesis have been described [49].…”

Section: Discussionmentioning

confidence: 99%

Adult Trees Cryptomeria japonica (Thunb. ex L.f.) D. Don Micropropagation: Factors Involved in the Success of the Process

Rojas-Vargas

Montalbán²,

Moncaleán³

2023

Forests

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Cryptomeria japonica (Thunb. ex L.f.) D. Don is a commercial tree native to Japan and is one of the most important forest species in that country and the Azores (Portugal). Because of the quality of C. japonica timber, several genetic improvement programs have been performed. Recently, some studies focusing on C. japonica somatic embryogenesis have been carried out. However, in this species, this process uses immature seeds as initial explants, and for this reason, it is not possible to achieve the maximum genetic gain (100% genetic of the donor plant). Although some studies have been made applying organogenesis to this species, the success of the process in adult trees is low. For this reason, our main goal was to optimize the micropropagation method by using trees older than 30 years as a source of plant material. In this sense, in a first experiment, we studied the effect of different types of initial explants and three basal culture media on shoot induction; then, two sucrose concentrations and two light treatments (LEDs versus fluorescent lights) were tested for the improvement of rooting. In a second experiment, the effects of different plant growth regulators (6-benzylaminopurine, meta-topolin, and thidiazuron) on shoot induction and the subsequent phases of the organogenesis process were analyzed. The cultures produced the highest number of shoots when QL medium (Quoirin and Lepoivre, 1977) and long basal explants (>1.5 cm) were used; the shoots obtained produced a higher number of roots when they were grown under red LED lights. Moreover, root induction was significantly higher in shoots previously induced with meta-topolin.

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