Role of Mesenchymal Stem Cell Therapy in Cisplatin Induced Nephrotoxicity in Adult Albino Rats: Ultrastructural &amp; Biochemical Study

Ghaly, Emad Nagiwub; Gergis, Safwat Wadie; Aziz, Joseph Naeim; Yassa, Hanan Dawood; Hassan, Hassan Abdel‐Raheem

doi:10.5530/ami.2014.2.3

Cited by 11 publications

(10 citation statements)

References 36 publications

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“…This was accompanied by the presence of pyknotic or karyolitic nuclei. This was in accordance with some previous researches [17,20] . It was demonstrated that cisplatin caused direct tubular injury through multiple interacting mechanisms.…”

Section: Discussionsupporting

confidence: 80%

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Role of Mesenchymal Stem Cells Versus their Conditioned Medium on Cisplatin-Induced Acute Kidney Injury in Albino Rat. A Histological and Immunohistochemical Study

Zaher

Shawarby

Hammouda

et al. 2017

Egyptian Journal of Histology

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Introduction: Acute kidney injury (AKI) is a syndrome of rapidly declining renal function. Cisplatin nephrotoxicity is a major cause of AKI in about one third of patients under cisplatin treatment. Stem cell therapy have been suggested as a protective measure against cisplatin-induced AKI. The conditioned medium of the stem cells (serum-free culture medium) has been also found to minimize renal injury and might develop a new therapeutic strategy that avoids stem cells administration. Aim of the Study:This study was conducted to compare the effect of intravenous injection of bone marrow-derived mesenchymal stem cells (BMSCs) versus their conditioned medium (CM) in minimizing the cisplatin-induced acute renal injury. Material and Methods: Sixty adult female albino rats were divided into 4 main groups. Group (I) served as a control group, Group (II) (cisplatin treated group) that were subdivided into two subgroups IIa and IIb, Group (III) (BMSCstreated group) and Group (IV) (CM-treated group). Five young male rats were additionally used for obtaining the BMSCs. Rats of all groups were sacrificed on 4th day of the experiment, except for the rats of subgroup (IIa (which were sacrificed after one day of cisplatin administration. Renal specimens were prepared for histological and immunohistochemical techniques. Morphometrical studies and statistical analysis were performed. Results: Treatment by BMSCs resulted in obvious improvement of renal structure with significant increase in Proliferating Cell Nuclear Antigen (PCNA). However, conditioned medium (CM) was less effective in treatment of acute AKI. Conclusion: BMSCs administration is preferable than their CM in reversing the acute structural damage of the kidney induced by cisplatin.

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Section: Discussionsupporting

confidence: 80%

“…It was reported that accumulation of glycoproteins due to renal damage and up-regulation of integrin, laminin and fibronectin were responsible for basement membrane thickening [20] .…”

Section: Discussionmentioning

confidence: 99%

Role of Mesenchymal Stem Cells Versus their Conditioned Medium on Cisplatin-Induced Acute Kidney Injury in Albino Rat. A Histological and Immunohistochemical Study

Zaher

Shawarby

Hammouda

et al. 2017

Egyptian Journal of Histology

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“…Both of them had been carefully assessed and scored in our study. In line with some other reports [35,16], significant reduction has been found in both stem cell and antioxidant groups than in the cisplatin group and this was further proved and fortified via immunohistochemical assay of α-SMA and Ki-67 in renal tissue. Both α-SMA and Ki-67 contents were significantly lower in renal tissue denoting decreased myelofibrosis, collagen formation and cell proliferation in the stem cell group and antioxidant group more than cisplatin group.…”

Section: Discussionsupporting

confidence: 74%

“…Either stem cells or antioxidant has reversed the increased level of level to a considerable extent, thereby confirming their inhibition of lipid peroxidation in cisplatin induced acute kidney injury. The ability of either stem cells or antioxidant in inhibition of lipid peroxidation and their potent role as free radical scavengers was stated before in other reports [35,38].…”

Section: Discussionmentioning

confidence: 98%

Effect of Antioxidants and Mesenchymal Stem Cells on Cisplatin Induced Renal Fibrosis in Rats

Nabil¹

2016

JSRT

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“…This might be due to up-regulation of integrins, laminin and fibronectin with subsequent increase in basement membrane thickness [14] . However, some authors found no changes in basement membrane in cisplatin induced renal injury [45] .…”

Section: Discussionmentioning

confidence: 99%

Transplanted Adipose Derived Mesenchymal Stem Cells Attenuate The Acute Renal Injury Induced by Cisplatin in Rats

Sakr

Elhai

Zeid

et al. 2017

Egyptian Journal of Histology

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Background: Traditional therapeutic strategies used for the treatment of acute kidney injury (AKI) proved to be less effective in reducing the morbidity and mortality rate. Recently, stem cell therapy showed a promise for treatment of this complex disorder. Aim: To investigate the therapeutic role of adipose-derived mesenchymal stem cells (AD-MSCs) in treatment of cisplatininduced nephrotoxicity as a model of AKI. Material and Methods: Twenty adult female Wister rats were divided equally into four groups. Group I was the control, the other three groups received a single intraperitoneal injection of cisplatin (5 mg /kg), where group II were sacrificed after one day from cisplatin injection, group III were sacrificed after seven days from cisplatin injection and group IV received adult male rat AD-MSCs (2x106 cells/rat) in tail vein one day after cisplatin injection and were sacrificed seven days after cisplatin injection. Results: The histopathological changes in the renal cortex were more obviously detected in group III than in group II. These changes include congested and shrunken glomeruli, dilated Bowman's space and loss of proximal convoluted tubules brush borders. Moreover, distal tubular cells showed cytoplasmic vacuolization, with pyknotic nuclei and presence of intraluminal hyaline casts. Interstitial collagen deposition was also noticed. In group IV, AD-MSCs administration almost restores the renal histological architecture. Increased tubular cell proliferation with marked reduction of the interstitial inflammation and fibrosis were also detected. However, some renal glomeruli and tubules showed degenerative changes. Male rat derived-stem cells were detected in the female kidney tissue by Y chromosome PCR technique. Conclusion: Administration of AD-MSCs had a potential regenerative effect for the management of AKI.

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Role of Mesenchymal Stem Cell Therapy in Cisplatin Induced Nephrotoxicity in Adult Albino Rats: Ultrastructural & Biochemical Study

Cited by 11 publications

References 36 publications

Role of Mesenchymal Stem Cells Versus their Conditioned Medium on Cisplatin-Induced Acute Kidney Injury in Albino Rat. A Histological and Immunohistochemical Study

Role of Mesenchymal Stem Cells Versus their Conditioned Medium on Cisplatin-Induced Acute Kidney Injury in Albino Rat. A Histological and Immunohistochemical Study

Effect of Antioxidants and Mesenchymal Stem Cells on Cisplatin Induced Renal Fibrosis in Rats

Transplanted Adipose Derived Mesenchymal Stem Cells Attenuate The Acute Renal Injury Induced by Cisplatin in Rats

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