Role of membrane proteins in thermal damage and necrosis of red blood cells

Ivanov, Ivan T.; Brähler, M.; Georgieva, Radostina; Bäumler, Hans

doi:10.1016/j.tca.2007.01.020

Cited by 20 publications

(16 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Table 1. Table 2 The experimental data on cell decomposition in references [12][13][14][15][16][17][18][19] were used for calculation of thermodynamic variables. 5 The concentration of free vesicles increased after exposure to heat stress from (1.47)×10 6 to (3.87)×10 6 6 vesicles/μL (Fig.…”

Section: Thermodynamics Of Erythrocyte Vesiculation In the Human Casementioning

confidence: 99%

Thermodynamic evaluation of vesicles shed by erythrocytes at elevated temperatures

Vodyanoy

2015

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Section: Thermodynamics Of Erythrocyte Vesiculation In the Human Casementioning

confidence: 99%

Thermodynamic evaluation of vesicles shed by erythrocytes at elevated temperatures

Vodyanoy

2015

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

“…Non-radiative relaxation to the ground state results in heat production. Localized heating can increase the permeability of the cell membrane by a local phase transition of the lipid bilayer or by thermal denaturation of integral proteins [74,86]. However, it was reported that photothermal heating by single photon absorption alone is not sufficient to effectively form pores in cell membranes [82,83,87].…”

Section: Photothermal Pore Formationmentioning

confidence: 99%

“…Of special relevance is ionization of water molecules and the generation of ROS [73,136,137]. ROS and free radicals can initiate a damaging chain reaction of lipid peroxidation followed by decreased hydrophobicity of the lipid bilayer [74,86]. Baumgart et al made use of this process by irradiating 100 nm AuNPs with a fs laser (45 fs pulse, λ = 810 nm) at a laser fluence of 60 mJ/cm 2 [135].…”

Section: Photochemical-induced Photoporationmentioning

confidence: 99%

Laser-assisted photoporation: fundamentals, technological advances and applications

Xiong

Samal

Demeester

et al. 2016

Advances in Physics: X

104

View full text Add to dashboard Cite

Laser-assisted photoporation is a promising technique that is receiving increasing attention for the delivery of membrane impermeable nanoscopic substances into living cells. Photoporation is based on the generation of localized transient pores in the cell membrane using continuous or pulsed laser light. Increased membrane permeability can be achieved directly by focused laser light or in combination with sensitizing nanoparticles for higher throughput. Here, we provide a detailed account on the history and current state-ofthe-art of photoporation as a physical nanomaterial delivery technique. We first introduce with a detailed explanation of the mechanisms responsible for cell membrane pore formation, following an overview of experimental procedures for realizing direct laser photoporation. Next, we review the second and most recent method of photoporation that combines laser light with sensitizing NPs. The different mechanisms of pore formation are discussed and an overview is given of the various types of sensitizing nanomaterials. Typical experimental setups to achieve nanoparticlemediated photoporation are discussed as well. Finally, we discuss the biological and therapeutic applications enabled by photoporation and give our current view on this expanding research field and the challenges and opportunities that remain for the near future.

show abstract

“…The images were taken with a confocal laser scanning microscope (LSM 510, Zeiss, Jena, Germany). calorimetric A peak) and the integral domain of band 3 (the calorimetric C peak), respectively (Brandts et al 1977;Ivanov et al 2007). Hence, it could be assumed that the peaks at the T A and T C top temperatures on the spectrophotometric (Fig.…”

Section: Thermal Denaturation Of Spectrin and Band 3 Of Triton--x-100mentioning

confidence: 99%

Effects of heat and freeze on isolated erythrocyte submembrane skeletons

Ivanov¹,

Paarvanova²,

Ivanov³

et al. 2017

gpb

Self Cite

View full text Add to dashboard Cite

Abstract. In this study we heated insoluble residues, obtained after Triton-X-100 (0.1 v/v%) extraction of erythrocyte ghost membranes (EGMs). Specific heat capacity, electric capacitance and resistance, and optical transmittance (280 nm) sustained sharp changes at 49°C (T A ) and 66°C (T C ), the known denaturation temperatures of spectrin and band 3, respectively. The change at T A was selectively inhibited by diamide (1 mM) and taurine mustard (1 mM) while its inducing temperature was selectively decreased by formamide in full concert with the assumed involvement of spectrin denaturation. In the residues of EGMs, pretreated with 4,4'-diiso-thiocyanato stilbene-2,2'-disulfonic acid (DIDS), the change at T C was shifted from 66 to 78°C which indicated the involvement of band 3 denaturation. The freeze and rapid thaw of EGM residues resulted in a strong reduction of cooperativity of band 3 denaturation while the slow thaw completely eliminated the peak of this denaturation. These effects of freeze-thaw were prevented in residues obtained from DIDS-treated EGMs. The freeze-thaw of residues slightly affected spectrin denaturation at 49°C although an additional denaturation appeared at 55°C. The results indicate preserved molecular structure and dynamics of the membrane skeleton in Triton-X-100 extracts of EGMs. The freeze-thaw inflicted strong damage on band 3 and spectrin-actin skeleton of EGM extracts which is relevant to cryobiology, cryosurgery and cryopreservation of cells.

show abstract

Role of membrane proteins in thermal damage and necrosis of red blood cells

Cited by 20 publications

References 27 publications

Thermodynamic evaluation of vesicles shed by erythrocytes at elevated temperatures

Thermodynamic evaluation of vesicles shed by erythrocytes at elevated temperatures

Laser-assisted photoporation: fundamentals, technological advances and applications

Effects of heat and freeze on isolated erythrocyte submembrane skeletons

Contact Info

Product

Resources

About