Role of Matrix Metalloproteinase-7 in Colorectal Adenomas

Kırımlıoğlu, Hale; Kırımlıoğlu, Vedat; Yilmaz, Sezai; Sağır, Vedat; Çoban, Sacit; Türkmen, Emine; Hilmioğlu, Fatih

doi:10.1007/s10620-005-9070-4

Cited by 20 publications

(21 citation statements)

References 18 publications

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“…MMP-7 is involved in the progression of human rectal cancer and is a key player in invasion and metastasis, and thus, its assessment may become useful for clinical diagnosis and predicting the prognosis of rectal cancer. 20,21 The correlation of MMP-7 expression with liver metastasis has been also reported. 22 It is synergistically upregulated by the oncogenic transcription factors activator protein (AP)-1 and b-catenin.…”

Section: Discussionmentioning

confidence: 95%

Effects of Monascus-Fermented Rice Extract on Malignant Cell—Associated Neovascularization and Intravasation Determined Using the Chicken Embryo Chorioallantoic Membrane Model

Hsu

et al. 2010

Integr Cancer Ther

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The traditional Chinese medicine, Hong-Qu, also called red mold rice in the United States and Europe, is used for treating blood stasis, a disorder related to hyperlipidemia and atherosclerosis. In addition to improving metabolic syndrome, extracts from Monascus-fermented rice inhibit the proliferation of various cancer cells in vitro and in vivo. The objective was to examine the effect of red mold rice ethanol extract (RMRE) on angiogenesis, invasion, and metastasis during tumor progression. RMRE significantly inhibited the proliferation of SW480 and SW620 human colorectal carcinoma cells in a dose-and time-dependent manner by using the MTT assay. A capillary-like network morphology was observed after the addition of 20 ng/mL vascular endothelial growth factor or SW620 culture-conditional medium, which was not seen after RMRE treatment. Moreover, spontaneous intravasation into Matrigel grafts of SW620 cells from the upper to the lower layers in the chick embryo chorioallantoic membrane (CAM) model was detected by the polymerase chain reaction (PCR) amplification of human Alu genomic DNA from the lower CAMs in the RMRE-untreated group. Neovascularization increased to 75.3% ± 11.6% by SW620 cells onplant with Matrigel grafts in the CAM model. However, RMRE significantly reduced CAM neovascularization in a dose-dependent manner. Finally, RMRE effectively decreased the activity of matrix metalloproteinase (MMP)-7 as determined by reverse transcription PCR (RT-PCR), Western blotting, and casein zymography assays. In summary, Monascus-fermented products exert a potent effect on tumor growth and activation, suggesting that they may serve as supplementary agents in adjuvant cancer therapy.

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Section: Discussionmentioning

confidence: 95%

Effects of Monascus-Fermented Rice Extract on Malignant Cell—Associated Neovascularization and Intravasation Determined Using the Chicken Embryo Chorioallantoic Membrane Model

Hsu

et al. 2010

Integr Cancer Ther

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“…3 Previous study suggests that MMP-7 proteins are mainly expressed in colon cancer cells and strongly associated with tumor progression. 5,6 A high plasma level of leptin was correlated with tumor progression in colon cancer patients. 7À9 Studies suggest that leptin could play crucial roles in different clinical stages of CRC.…”

Section: Introductionmentioning

confidence: 99%

Cancer Chemopreventive Effects of Lycopene: Suppression of MMP-7 Expression and Cell Invasion in Human Colon Cancer Cells

Lin

Wang

Kuo

et al. 2011

J. Agric. Food Chem.

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Clinical studies indicate that high blood levels of leptin or matrix metalloproteinase-7 (MMP-7; matrilysin) proteins are associated with tumor progression of human colorectal cancer (CRC). Leptin could play an important role in cell migration and invasion of cancer cells. Our previous study indicated that lycopene could inhibit the proliferation of human colon cancer cells in vitro. However, the inhibitory effects of lycopene on the progression of human colon cancer cells have not been demonstrated yet. In this study, we investigated the inhibitory effects of lycopene on tumor progression including cell invasion and MMP-7 expression in leptin-stimulated human colon cancer cells in vitro. Our results demonstrated that lycopene significantly inhibited leptin-mediated cell invasion and MMP-7 expression in human colon cancer HT-29 cells. Lycopene could augment the expression and stability of E-cadherin proteins. Our results showed that MAPK/ERK and PI3K/Akt signaling pathways played important roles in leptin-mediated MMP-7 expression and cell invasion. Lycopene could effectively inhibit the phosphorylation of Akt, glycogen synthase kinase-3β (GSK-3β) and ERK 1/2 proteins. The molecular mechanisms of lycopene were in part through decreases in nuclear levels of AP-1 and β-catenin proteins. These novel findings suggested that lycopene could act as a chemopreventive agent to suppress MMP-7 expression and leptin-mediated cell invasion in human colon cancer HT-29 cells.

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“…A similar trend was observed for TNF· expression with the highest expression (+80-fold) in the largest most advanced tumors (225-470 mm 3 ). In humans, an increased expression of matrix metalloproteinases (MMP) genes has been associated with early stages of tumor development (15). Here, we observe that MMP-7 gene expression was already enhanced in small polyps (+210-fold) compared to the level found in the mucosal samples.…”

Section: Resultsmentioning

confidence: 76%

Identification of gene expression profiles correlated to tumor progression in a preclinical model of colon carcinogenesis

Bousserouel

Kauntz²,

Gossé³

et al. 2010

Int J Oncol

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Abstract. The rat azoxymethane (AOM)-induced colon carcinogenesis model provides useful information for understanding human colorectal neoplasia. Here, we used the AOM model to measure the gene expression profiles of biomarkers related to tumor progression. We assessed tumor progression stages by computed tomographic (CT) colonography. Messenger RNAs were isolated from tumors and mucosal samples, and gene expression levels were assessed by realtime quantitative polymerase chain reaction (PCR). We show that early stages of tumor progression are associated with an upregulation of matrix metalloproteinase-7 (MMP-7) and of genes involved in the inflammatory response, including interleukin (IL1ß) and tumor necrosis factor-· (TNF·). The ratio of B-cell lymphoma/leukemia 2 (Bcl-2)-associated X proteins (Bax) to Bcl-2 transcript (proapototic/antiapoptotic signals) is elevated in early stages of tumor progression (Bax/Bcl-2 >1) and reversed in more advanced stages of tumor development (Bax/Bcl-2 <1). These changes are associated with the reduced expression of TNF-related apoptosis-inducing ligand (TRAIL)-death receptor 5 (DR5) and FAS (also known as CD95) apoptotic receptors. Advanced stages of tumor development are characterized by an increase in MMP-9 expression associated with the upregulation of components of the innate immune system: ·-defensin 5 (DEF-5) and neutrophil gelatinaseassociated lipocalin (NGAL). The identification of specific gene expression profiles that correlate with tumor progression stages, as reported in the present study, may represent an important step in evaluating potential chemopreventive and/ or chemotherapeutic agents prior to initiating clinical trials.

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Role of Matrix Metalloproteinase-7 in Colorectal Adenomas

Cited by 20 publications

References 18 publications

Effects of Monascus-Fermented Rice Extract on Malignant Cell—Associated Neovascularization and Intravasation Determined Using the Chicken Embryo Chorioallantoic Membrane Model

Effects of Monascus-Fermented Rice Extract on Malignant Cell—Associated Neovascularization and Intravasation Determined Using the Chicken Embryo Chorioallantoic Membrane Model

Cancer Chemopreventive Effects of Lycopene: Suppression of MMP-7 Expression and Cell Invasion in Human Colon Cancer Cells

Identification of gene expression profiles correlated to tumor progression in a preclinical model of colon carcinogenesis

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