Role of matrix metalloproteinase-10 in the BMP-2 inducing osteoblastic differentiation

Li, Mao; Yano, Masato; Kawao, Naoyuki; Tamura, Yukinori; Okada, Kiyotaka; Kaji, Hiroshi

doi:10.1507/endocrj.ej13-0270

Cited by 21 publications

(21 citation statements)

References 36 publications

(35 reference statements)

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“…Some of the matrix metalloproteinases can augment the expression of the osteogenic genes such as osterix, type 1 collagen, alkaline phosphatase, and osteocalcin [15]. There are few reports about how metformin affects matrix metalloproteinases in osteoblast under high glucose.…”

Section: Discussionmentioning

confidence: 99%

Metformin Rescues the MG63 Osteoblasts against the Effect of High Glucose on Proliferation

Shao

Cao

Song

et al. 2014

Journal of Diabetes Research

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Aims. To study the proliferation of osteoblasts and genes expression under normal glucose, high glucose, and metformin (Met). Methods. MG63 osteoblast-like cells were cultured in osteogenic medium supplemented with normal glucose (glucose 5.5 mmol/L) or high glucose (glucose 16.7 mmol/L) and metformin + high glucose (Met 300 μmol/L + glucose 16.7 mmol/L). Proliferation was detected with CCK-8 assay at days 1, 3, and 7. Real-time PCR and Western blot were performed to compare the expression of collagen I (Col I), osteocalcin (OCN), osteoprotegerin (OPG), receptor activator for NF-κB ligand (RANKL), and metal matrix proteinases 1 and 2 (MMP1, MMP2). Alkaline phosphatase (ALP) activity was also detected at days 6, 12, and 18. Results. Exposure to high glucose inhibited the proliferation of osteoblasts (P < 0.05), with suppressed OCN and OPG. Meanwhile, Col I, RANKL, MMP1, and MMP2 were unaffected. Metformin attenuated the suppression on proliferation with increased expression of Col I, OCN, and OPG, meanwhile suppressing MMP1 and MMP2. High glucose lowered the intracellular ALP, while metformin raised it. Metformin attenuated the downregulation of ALP completely at day 6, partly at day 12, but not at day 18. Conclusions. Metformin attenuated the suppression effect of high glucose to the osteoblast proliferation and gene expression, more prominently in earlier stage.

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Section: Discussionmentioning

confidence: 99%

Metformin Rescues the MG63 Osteoblasts against the Effect of High Glucose on Proliferation

Shao

Cao

Song

et al. 2014

Journal of Diabetes Research

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“…60 Conversely, in transfected mouse myoblastic C2C12 cells producing MMP-10, which is an enzyme that cleaves matrix molecules, the levels of type 1 collagen, ALP, and osteocalcin mRNA were increased. 61 Another possible explanation for the lower expression of collagen I and ALP in cells on O-terminated surfaces is a higher proliferative activity (stimulation) of cells on these surfaces. The proliferation activity has often been reported to be inversely correlated to the cell differentiation, which includes the expression of collagen, ALP, and osteocalcin.…”

mentioning

confidence: 99%

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Lišková¹,

Babchenko²,

Varga³

et al. 2015

IJN

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Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings.

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“…BMP-2 is an osteogenic induction factor, which can facilitate osteoblastic differentiation and regulate the occurrence of heterotopic ossification. BMP-2 expression level was shown to be related to bone formation level (17). TGF-β regulates cells proliferation and differentiation, increases the synthesis of extracellular matrix and facilitates cartilage tissue collagen synthesis (18).…”

Section: Discussionmentioning

confidence: 99%

Local influence of high molecular polyethylene particles on heterotopic ossification

Zeng¹,

Zhu²,

Wang³

et al. 2017

Experimental and Therapeutic Medicine

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We studied the effect of molecular polyethylene particles on local heterotopic ossification. A total of 36 healthy Sprague-Dawley rats were randomly divided into the control group (n=18) and the observation group (n=18). High molecular polyethylene particles were injected to rupture Achilles tendon position in the observation group, and normal saline was injected in the control group. X-ray examinations were conducted on Achilles tendon in the 4th, 8th and 12th week after operation. The incidence rate of heterotopic ossification was evaluated, and bone trabecula morphological structure was studied under optical microscope after hematoxylin and eosin staining. Bone morphogenetic protein 2 (BMP-2), transforming growth factor-β (TGF-β), interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α), runt-related transcription factor 2 (Runx2) and matrix metalloproteinase-9 (MMP-9) expression levels were also measured. Our results showed that heterotopic ossification incidence in the observation group was significantly lower than that in the control group. Achilles tendon structure in the control group increased in volume, and its texture was harder and cartilage-like. In the observation group, trabecular bone volume, thickness and quantity were more than those observed in the control group. BMP-2, TGF-β, IL-1, TNF-α, Runx2 and MMP-9 levels in the observation group were significantly lower than those in the control group. We concluded that, high molecular polyethylene particles had a significant inhibiting effect on local heterotopic ossification.

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Role of matrix metalloproteinase-10 in the BMP-2 inducing osteoblastic differentiation

Cited by 21 publications

References 36 publications

Metformin Rescues the MG63 Osteoblasts against the Effect of High Glucose on Proliferation

Metformin Rescues the MG63 Osteoblasts against the Effect of High Glucose on Proliferation

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Local influence of high molecular polyethylene particles on heterotopic ossification

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