Role of maternal exposures and newborn genotypes on newborn chromosome aberration frequencies

Pluth, Janice M.; Ramsey, Marilyn J.; Tucker, James D.

doi:10.1016/s1383-5718(99)00217-x

Cited by 62 publications

(39 citation statements)

References 40 publications

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“…Maternal cigarette smoking during pregnancy and a possible transplacental effect on the fetal development were the subject matter of numerous investigations (Pluth et al 2000;Neri et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…It is known that human fetus reacts to different environmental factors that may cross the placental barrier including the medicaments administered to their mothers during pregnancy for therapeutic or prophylactic reasons (Pluth et al 2000). Because of intensive proliferate activity of embryonic and fetal tissue, the influence of xenobiotics on the induction of DNA damages is increased.…”

Section: Methodsmentioning

confidence: 99%

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Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

Grujičić

Milošević-Djordjević

Arsenijevic

et al. 2008

Tohoku J. Exp. Med.

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In prevention of preterm labor, betamimetics are used in gynecological practice mostly combined with antiarrhythmic verapamil because of their therapeutic cardiovascular side effects. The aim of this study was to investigate the influence of a betamimetic (ritodrine hydrochloride, fenoterol or hexoprenaline) and verapamil (administered to mothers) on the frequency of micronuclei (MN) in umbilical cord blood lymphocytes of neonates, using cytokinesis-block micronucleus test. The analyzed sample included 23 babies whose mothers received the therapy and 30 control babies whose mothers received no therapy. The average MN frequency was significantly higher in the neonates whose mothers received the therapy (8.13 ± 2.69 MN/1000 BN cells), in comparison with the baseline frequency in untreated controls (3.30 ± 2.63 MN/1000 BN cells), with probability p < 0.05. The highest MN frequency was found in neonates whose mothers received fenoterol and verapamil (2.8-fold i.e. 9.10 ± 3.00 MN/1000 BN cells), while ritodrine hydrochloride and hexoprenaline combined with verapamil induced 2.3-fold and 2.2-fold higher MN values than in controls (7.50 ± 3.33 MN/1000 BN cells and 7.29 ± 0.95 MN/1000 BN cells). Multiple linear regression analysis showed that MN frequency was affected only by the maternal therapeutic treatment, while the neonates' sex, maternal age, cigarette smoking, and therapeutic doses did not affect the MN frequency in umbilical lymphocytes of neonates. We conclude that the treatment of pregnant women with a betamimetic and verapamil significantly increases the MN frequency in umbilical cord blood lymphocytes of neonates, regardless to therapeutic doses.umbilical cord blood lymphocytes; micronuclei; betamimetic; verapamil.Tohoku

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

Grujičić

Milošević-Djordjević

Arsenijevic

et al. 2008

Tohoku J. Exp. Med.

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“…Cigarette smoke has been linked to an increased frequency of chromosomal abnormalities and aneuploidy in sperm (Pluth et al, 2000). The rate of adolescent addiction to cigarettes and tobacco use is high in India.…”

Section: Introductionmentioning

confidence: 99%

Tobacco Use Increases Oxidative DNA Damage in Sperm - Possible Etiology of Childhood Cancer

Kumar

Chawla²,

Bisht³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Background: Cigarette smoking and tobacco chewing are common modes of consuming tobacco all over the world. Parents need to be aware that germ cell integrity is vital for birth of healthy offspring as biological parenting begins much before birth of a child and even before conception. The present study was conducted to determine the etiology of non-familial sporadic heritable retinoblastoma (NFSHRb), by evaluating oxidative sperm DNA damage in fathers due to use of tobacco (smoking and chewing). Materials and Methods: We recruited 145 fathers of NFSHRb children and 53 fathers of healthy children (controls) in the study. Tobacco history was obtained by personal interview. Seminal reactive oxygen species (ROS) in semen, sperm DNA fragmentation index (DFI) and 8 hydroxy 2' deoxyguanosine (8-OHdG) levels in sperm were evaluated. The RB1 gene was screened in genomic blood DNA of parents of children with NFSHRb and controls. Odds ratios (ORs) derived from conditional logistic regression models. Results: There was significant difference in the levels of ROS (p<0.05), DFI (p<0.05) and 8-OHdG (p<0.05) between tobacco users and non-users. The OR of NFSHRb for smokers was 7.29 (95%CI 2.9-34.5, p<0.01), for tobacco chewers 4.75 (2.07-10.9, p<0.05) and for both 9.11 (3.79-39.2; p<0.01). Conclusions: This study emphasizes the adverse effect of tobacco on the paternal genome and how accumulation of oxidative damage in sperm DNA may contribute to the etiology of NFSHRb. In an ongoing parallel study in our laboratory, 11 of fathers who smoked underwent. Meditation and yoga interventions, showed significant decline in levels of highly mutagenic oxidised DNA adducts after 6 months. Thus our lifestyle and social habits impact sperm DNA integrity and simple interventions like yoga and meditation are therapeutic for oxidative damage to sperm DNA.

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“…They have been used widely as a biomarker of cancer risk in adults [Brogger et al, 1990;Chorazy, 1996;Motykiewicz et al, 1998;Huttner et al, 1999;Michalska et al, 1999], and to measure of the effect of environmental pollutants in infants and children [Ramsey et al, 1995;Sram et al, 1999;Pluth et al, 2000;Rossner et al, 2002;Neri et al, 2006a,b].…”

Section: Introductionmentioning

confidence: 99%

“…Previous research has utilized chromosome aberrations as a tool to examine the relationship between in utero environmental exposures and cancer risk [Ramsey et al, 1995;Sram et al, 1999;Pluth et al, 2000;Rossner et al, 2002]. Two studies using fluorescence in situ hybridization (FISH) as a detection method were able to demonstrate a significant positive association between active maternal smoking during pregnancy and increased chromosome aberration levels measured in cord blood [Ramsey et al, 1995;Pluth et al, 2000]. Another two studies performed Giemsa-staining instead of FISH and were not able to demonstrate the effect of air pollution on levels of aberrant cells.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence in situ hybridization is necessary to detect an association between chromosome aberrations and polycyclic aromatic hydrocarbon exposure in utero and reveals nonrandom chromosome involvement

Bocskay

Orjuela

Tang

et al. 2007

Environ and Mol Mutagen

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Chromosome aberrations are associated with environmental exposures in infants and children. Recently we reported that prenatal exposure to airborne polycyclic aromatic hydrocarbons (PAHs) was significantly (P < 0.01) associated with stable aberration frequencies in cord blood from a subset of 60 newborns from the Columbia Center for Children's Environmental Health Prospective Cohort Study (Bocskay K et al. [2005]: Cancer Epidemiol Biomarkers Prev 14:506-511). To determine whether the environmental exposures may be targeting specific chromosomes and to compare various methods for measuring chromosome aberrations, we further evaluated this same subset of subjects composed of African-American and Dominican nonsmoking mother-newborn pairs residing in low-income neighborhoods of New York City, and exposed to varying levels of airborne PAHs. Chromosome aberrations were measured in cord blood lymphocytes, both by whole chromosome probe (WCP) fluorescence in situ hybridization (FISH) and traditional Giemsa-staining. Prenatal exposures were assessed by personal air monitoring. Breaks in chromosomes 1-6, as detected by WCP FISH, were nonrandomly distributed, underscoring the importance of appropriate chromosome probe selection to capture cytogenetic damage in response to exposure. FISH for stable aberrations was found to be a more sensitive method for detecting aberration frequencies associated with environmental exposures, when compared with FISH for unstable aberrations or Giemsa-staining for aberrations. Together, these results suggest that PAHs may be targeting specific chromosomes and highlight the importance of using the more sensitive detection methods to assess risk in populations with low levels of exposure.

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Role of maternal exposures and newborn genotypes on newborn chromosome aberration frequencies

Cited by 62 publications

References 40 publications

Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

Tobacco Use Increases Oxidative DNA Damage in Sperm - Possible Etiology of Childhood Cancer

Fluorescence in situ hybridization is necessary to detect an association between chromosome aberrations and polycyclic aromatic hydrocarbon exposure in utero and reveals nonrandom chromosome involvement

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