Role of mast cells in cow metritis

Abstract

Introduction: Bovine postpartum metritis causes great losses. Mast cell (MC)-released mediators participate in uterine inflammation and immune response, but their role in postpartum metritis in cows has not been reported. This study investigated the effect of endometrial MC on the disorder. Material and Methods: Ten dairy cows, at 6 to 10 days postpartum and with acute purulent metritis made up the experimental group, and 10 comparable healthy cows the control group. Endometrial histamine and IgE levels were determined by ELISA, and the MC particle state and expression of histamine H1 (H1R) and H2 (H2R) mRNA receptors were examined by transmission electron microscope and real-time quantitative PCR, respectively. Results: Endometrial histamine and IgE levels were significantly higher in the experimental group. In the control group, homogenously distributed size-varied granules were seen in MC cytoplasm of endometrium of lamina propria. In the experimental group however, these showed degranulation with features of reduction. The level of H1R mRNA was lower in the experimental group, but that of H2R mRNA was higher. Conclusion: The results suggest MC type I hypersensitivity characteristics during metritis, and histamine provocation of local inflammation. High expression of H2R and low expression of H1R inhibited the inflammatory response and prevented excessive uterine tissue damage.

The role of leukotriene B4 in cow metritis

“…Mast cells release pre-stored mediators such as histamine and tryptase after activation. This is consistent with the finding of our previous study that mast cells are activated and degranulated to release histamine in metritis ( 24 ). In addition to SP content, the content of leukotriene B 4 increased significantly in metritis, which is also the main new synthetic medium synthesised and released by mast cells after activation.…”

“…Mast cells (MC) are activated and degranulated to release pre-storage medium histamine in cow metritis ( 24 ). A type I hypersensitivity reaction of mast cells occurs during metritis, and local inflammation can cause histamine excitation.…”

Interaction between stress hormones and phagocytic cells and its effect on the health status of dairy cows: A review

“…Wang et al . [ 105 ] demonstrated that high expression of H2R and low expression of H1R in the endometrial tissue impaired the inflammatory immune response and prevented excessive uterine tissue damage in metritis cows. Sun et al .…”

“…H1R was reported in buffalo myometrium and found to have an important role in regulating myometrial contractility during normal and pathological states [104]. Wang et al [105] demonstrated that high expression of H2R and low expression of H1R in the endometrial tissue impaired the inflammatory immune response and prevented excessive uterine tissue damage in metritis cows. Sun et al [106] demonstrated that histamine increased the mRNA expressions and concentrations of inflammatory cytokines, including TNF-α, IL-6, and IL-1β, thereby inducing the inflammatory response of bovine rumen epithelial cells through the NF-κB pathway.…”