Role of Maize Root Exudates in Promotion of Colonization of Bacillus velezensis Strain S3-1 in Rhizosphere Soil and Root Tissue

Jin, Yeqing; Zhu, Hangfei; Luo, Si; Yang, Wenwu; Zhang, Li; Li, Shanshan; Jin, Qinian; Cao, Qin; Sun, Shili; Xiao, Ming

doi:10.1007/s00284-019-01699-4

Cited by 57 publications

(24 citation statements)

References 37 publications

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“…To further confirm that root exudates and exogenously-added organic acids affect biofilm formation of strain B26, quantitative microtiter assay showed that citric acid and oxalic acid promoted maximum biofilm formation after 48 h compared to the control, and most importantly, Brachypodium root exudate stimulated biofilm formation of strain B26. Similar results were reported where citric acid stimulated biofilm formation by Bacillus amyloliquefaciens SQR9 ( Zhang et al, 2014 ) and oxalic acid induced biofilm formation by Bacillus velezensis Strain S3-1 ( Jin et al, 2019 ).…”

Section: Discussionsupporting

confidence: 88%

“…The enhancement of biofilm formation in response to root exudates was previously reported in Bacillus velezensis strain S3-1 in maize ( Jin et al, 2019 ), Bacillus velezensis strain FZB42 in tomato ( Al-Ali et al, 2018 ), B. subtilis in Arabidopsis and tomato ( Rudrappa et al, 2008 ; Chen et al, 2012 ) and Bacillus amyloliquefaciens NJN-6 in banana root exudates ( Yuan et al, 2015 ). To further confirm that root exudates and exogenously-added organic acids affect biofilm formation of strain B26, quantitative microtiter assay showed that citric acid and oxalic acid promoted maximum biofilm formation after 48 h compared to the control, and most importantly, Brachypodium root exudate stimulated biofilm formation of strain B26.…”

Section: Discussionsupporting

confidence: 59%

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A Crosstalk Between Brachypodium Root Exudates, Organic Acids, and Bacillus velezensis B26, a Growth Promoting Bacterium

et al. 2020

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Plant growth-promoting rhizobacteria (PGPR) are associated with plant roots and use organic compounds that are secreted from root exudates as food and energy source. Root exudates can chemoattract and help bacteria to colonize the surface of plant roots by inducing chemotactic responses of rhizospheric bacteria. In this study, we show that root colonization of Brachypodium distachyon by Bacillus velezensis strain B26 depends on several factors. These include root exudates, organic acids, and their biosynthetic genes, chemotaxis, biofilm formation and the induction of biofilm encoding genes. Analysis of root exudates by GC-MS identified five intermediates of the TCA cycle; malic, fumaric, citric, succinic, oxaloacetic acids, and were subsequently evaluated. The strongest chemotactic responses were induced by malic, succinic, citric, and fumaric acids. In comparison, the biofilm formation was induced by all organic acids with maximal induction by citric acid. Relative to the control, the individual organic acids, succinic and citric acids activated the epsD gene related to EPS biofilm, and also the genes encoding membrane protein ( yqXM ) and hydrophobin component ( bslA ) of the biofilm of strain B26. Whereas epsA and epsB genes were highly induced genes by succinic acid. Similarly, concentrated exudates released from inoculated roots after 48 h post-inoculation also induced all biofilm-associated genes. The addition of strain B26 to wild type and to icdh mutant line led to a slight induction but not biologically significant relative to their respective controls. Thus, B26 has no effect on the expression of the ICDH gene, both in the wild type and the mutant backgrounds. Our results indicate that root exudates and individual organic acids play an important role in selective recruitment and colonization of PGPR and inducing biofilm. The current study increases the understanding of molecular mechanisms behind biofilm induction by organic acids.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 59%

A Crosstalk Between Brachypodium Root Exudates, Organic Acids, and Bacillus velezensis B26, a Growth Promoting Bacterium

et al. 2020

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“…Biofilms are the sessile cells embedded in a self-produced matrix of extracellular polymeric substance and attached to the plant roots [33]. By utilizing the root exudate as carbon and energy source, most of the rhizobacteria form biofilm on the surface of the plant root [34][35][36][37][38][39][40]. Yuan et al [40] also identified that the malic acid present in the maize root can trigger the biofilm-forming genes (epsD exopolysaccharide biosynthesis gene and yqxM, TasA proteinproducing gene) of rhizobacterial strain, Bacillus amyloliquefaciens NJN-6.…”

Section: Discussionmentioning

confidence: 99%

Harnessing PGPR inoculation through exogenous foliar application of salicylic acid and microbial extracts for improving rice growth

Bowya

Balachandar

2020

J Basic Microbiol

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Plant‐growth‐promoting rhizobacteria (PGPR) should effectively colonize along the plant root to enhance the plant and soil health. The present investigation aims to improve the PGPR‐mediated plant health benefits through above‐ground foliar management. A green fluorescent protein‐tagged PGPR strain, Pseudomonas chlororaphis (ZSB15‐M2) was inoculated in a nonautoclaved agricultural soil before rice culturing. Salicylic acid and cell extracts of Corynebacterium glutamicum and Saccharomyces cerevisiae as a supply of hormonal and inducer compounds were applied on the foliage of the 10‐days‐old rice plants and subsequently observed the colonizing ability of ZSB15‐M2. The cell extracts of Corynebacteria and yeast showed a 100‐fold increase in the ZSB15‐M2 population in the rhizosphere of rice, whereas salicylic acid had a 10‐fold increase in relation to mock control. The rice root exudates collected after the spraying of salicylic acid and microbial extracts showed significantly enhanced release of total carbon, total protein, total sugar, total amino nitrogen, total nitrogen, and phenol content. In vitro assays revealed that these root exudates collected after exogenous spray of these chemicals enhanced the chemotactic motility and biofilm formation of ZSB15‐M2 compared to the control plant's root exudate. Metabolomic analysis of root exudates collected from these rice plants by gas chromatography–mass spectrometry revealed that the Corynebacteria and yeast cell extracts enhanced the divergence of metabolites of rice root exudate. Further, due to these cumulative effects in the rice rhizosphere, the total chlorophyll, total protein, total nitrogen, and total phosphorus of rice were significantly improved. These observations provide insights into the rhizosphere functioning of rice plants as modulated by above‐ground treatments with improved colonization of inoculant strains as well as the plant growth.

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“…Moreover, plants are also able to disrupt the QS system by producing QS inhibitors, which either degrade QS signals or compete for signal receptors. For example, extracts from Medicago truncatula, cinnamon, grapefruit, and other edible plants and fruits showed QS inhibition activity against plant pathogens [94]. Bioactive molecules produced naturally by marine organisms and fungi and chemically synthesized compounds and antibodies have been reported to act as QS inhibitors.…”

Section: Microbe-microbe Interactionsmentioning

confidence: 99%

Plant–Microbiome Crosstalk: Dawning from Composition and Assembly of Microbial Community to Improvement of Disease Resilience in Plants

Noman

Ahmed

Ijaz

et al. 2021

IJMS

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Plants host diverse but taxonomically structured communities of microorganisms, called microbiome, which colonize various parts of host plants. Plant-associated microbial communities have been shown to confer multiple beneficial advantages to their host plants, such as nutrient acquisition, growth promotion, pathogen resistance, and environmental stress tolerance. Systematic studies have provided new insights into the economically and ecologically important microbial communities as hubs of core microbiota and revealed their beneficial impacts on the host plants. Microbiome engineering, which can improve the functional capabilities of native microbial species under challenging agricultural ambiance, is an emerging biotechnological strategy to improve crop yield and resilience against variety of environmental constraints of both biotic and abiotic nature. This review highlights the importance of indigenous microbial communities in improving plant health under pathogen-induced stress. Moreover, the potential solutions leading towards commercialization of proficient bioformulations for sustainable and improved crop production are also described.

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Role of Maize Root Exudates in Promotion of Colonization of Bacillus velezensis Strain S3-1 in Rhizosphere Soil and Root Tissue

Cited by 57 publications

References 37 publications

A Crosstalk Between Brachypodium Root Exudates, Organic Acids, and Bacillus velezensis B26, a Growth Promoting Bacterium

A Crosstalk Between Brachypodium Root Exudates, Organic Acids, and Bacillus velezensis B26, a Growth Promoting Bacterium

Harnessing PGPR inoculation through exogenous foliar application of salicylic acid and microbial extracts for improving rice growth

Plant–Microbiome Crosstalk: Dawning from Composition and Assembly of Microbial Community to Improvement of Disease Resilience in Plants

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