Role of macrophages in early protective immune responses induced by two vaccines against foot and mouth disease

Quattrocchi, Valeria; Langellotti, Cecilia; Pappalardo, Juan Sebastian; Olivera, Vivian; Giacomo, Sebastián Di; Rooijen, Nico van; Montagnoli, Claudia; Waldner, Claudia; Zamorano, Patricia

doi:10.1016/j.antiviral.2011.08.007

Cited by 33 publications

(48 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seraneutralizing antibodies (SN Abs) were measured as previously described [15]. Briefly: sera were serially diluted and dilutions were incubated with 100 TCID50/well of infectious FMDV.…”

Section: Anti-fmdv Ab Measurementsmentioning

confidence: 99%

See 1 more Smart Citation

Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity

Quattrocchi

Molinari

Langellotti

et al. 2013

Vaccine

Self Cite

View full text Add to dashboard Cite

“…Seraneutralizing antibodies (SN Abs) were measured as previously described [15]. Briefly: sera were serially diluted and dilutions were incubated with 100 TCID50/well of infectious FMDV.…”

Section: Anti-fmdv Ab Measurementsmentioning

confidence: 99%

“…Total antibodies against FMDV were assessed by ELISA as described previously [11][12][13]. Briefly, Immulon II plates were coated with anti-FMDV rabbit serum in 0.05 M carbonate-bicarbonate buffer, pH 9.6.…”

Section: Anti-fmdv Ab Measurementsmentioning

confidence: 99%

Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity

Quattrocchi

Molinari

Langellotti

et al. 2013

Vaccine

Self Cite

View full text Add to dashboard Cite

“…ADCP has been demonstrated as necessary for clearance of viral infections and has been understudied in the case of prevention of MTCT of HIV. [38][39][40][41][42] Given the paucity of knowledge about the potential contribution of ADCP activity by BM phagocytes to prevention of MTCT of HIV, we undertook the study described below of ADCP mediated by cells from BM obtained at 7-183 days postpartum.…”

Section: Introductionmentioning

confidence: 99%

Phagocytosis of a Model Human Immunodeficiency Virus Target by Human Breast Milk Leukocytes Is Predominantly Granulocyte-Driven When Elicited by Specific Antibody

Powell

Fox

Liu

et al. 2019

Breastfeeding Medicine

View full text Add to dashboard Cite

Background: Studies demonstrate a protective effect of antibodies (Abs) in breast milk (BM) against motherto-child transmission (MTCT) of human immunodeficiency virus (HIV). Contribution of the BM cellular component has been overlooked. The only clinical HIV vaccine trial to demonstrate efficacy, RV144, correlated protection with Abs mediating functions through the constant immunoglobulin region-the crystallizable fragment (Fc). These data support induction of vaccine Abs triggering antiviral activities by leukocytes through Fc receptors (FcRs). Objective: To measure Ab-dependent cellular phagocytosis (ADCP), an essential Fc-mediated response, by BM phagocytes. Materials and Methods: Cells were isolated from five human BM samples obtained at 7-183 days postpartum and analyzed for ADCP. Fluorescent beads coated with HIV envelope (Env) epitopes were used as targets. Sixty-seven to 100 mL of milk was utilized.Results: Total cell concentrations per milliliter were 16,083-222,857, with 1.6-12.3% being CD45 + leukocytes. ADCP activity was measurable using the HIV-specific Ab 830A. Use of the actin inhibitor cytochalasin D and FcR blocker indicated that ADCP was actin dependent and required FcR engagement. ADCP scores were variable, but largely consistent, across the samples studied, exhibiting <4-fold difference from lowest to highest activity for CD45 + cells. Of the CD45 + ADCP, significantly more activity was granulocyte derived (72-95%), while the remaining activity was monocyte driven. Conclusions: The data indicate that BM phagocytes can manifest antiviral activities in the presence of specific Abs and therefore may contribute to reduction of MTCT of HIV.

show abstract

“…Protection against FMDV was assessed as previously described [30]. Briefly, mice were IP inoculated with 10 2 TCID infectious FMDV A/Arg/01.…”

Section: Methodsmentioning

confidence: 99%

Transient Gene Expression in Serum-Free Suspension-Growing Mammalian Cells for the Production of Foot-and-Mouth Disease Virus Empty Capsids

et al. 2013

View full text Add to dashboard Cite

Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.

show abstract

Role of macrophages in early protective immune responses induced by two vaccines against foot and mouth disease

Cited by 33 publications

References 40 publications

Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity

Co-inoculation of baculovirus and FMDV vaccine in mice, elicits very early protection against foot and mouth disease virus without interfering with long lasting immunity

Phagocytosis of a Model Human Immunodeficiency Virus Target by Human Breast Milk Leukocytes Is Predominantly Granulocyte-Driven When Elicited by Specific Antibody

Transient Gene Expression in Serum-Free Suspension-Growing Mammalian Cells for the Production of Foot-and-Mouth Disease Virus Empty Capsids

Contact Info

Product

Resources

About