Role of Macrophages During early Achilles Tendon Healing

Blomgran, Parmis; Hammerman, Malin; Blomgran, Robert; Aspenberg, Per

doi:10.32098/mltj.01.2021.02

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Example references: Blomgran et al, 2017;Kataoka et al, 2018;Nakagaki et al, 2013;Ueda et al, 2019;Zhang et al, 2020. von Kossa -mineralised/calcified tissue Marlon Schneider recently published a comprehensive commentary on the von Kossa staining technique (Schneider, 2021). von Kossa staining is a two-step reaction with silver cations reacting firstly with calcium deposits, which are stained transiently in yellow.…”

Section: Masson-goldner Trichrome -Different Types Of Tissuementioning

confidence: 99%

Histology of tendon and enthesis – suitable techniques for specific research questions

Angrisani¹,

Willbold²,

Kampmann³

et al. 2022

eCM

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he musculoskeletal system consists of different components comprising a wide range of tissue types, with tendons being one part. Tendon degeneration or rupture have a high prevalence in all age groups, often with poor outcomes of surgical treatment such as chronic pain and high re-tear rates. Therefore, much effort has been directed to further develop diagnostic and therapeutic methods as well as reconstruction techniques, including using adequate placeholders or implants. Diagnostic approaches and advanced stages of preclinical studies will inevitably include histological examination of the pathologically affected tissue. The present study presents adequate tendon-related, histological techniques, including the embedding of soft- and hard-tissue samples in different media. Consideration is also given to samples containing residual implant materials or having been subjected to standard staining protocols and immunohistochemical procedures. The study further examines cells and tendon structure to detect degenerative, fibrotic or inflammatory conditions and possible foreign-body responses to implanted materials. Infraspinatus tendons from preclinical studies carried on rat and sheep samples, as well as human biceps tendon samples, have been used as example materials.

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Section: Masson-goldner Trichrome -Different Types Of Tissuementioning

confidence: 99%

Histology of tendon and enthesis – suitable techniques for specific research questions

Angrisani¹,

Willbold²,

Kampmann³

et al. 2022

eCM

View full text Add to dashboard Cite

show abstract

“…In contrast, mild attenuation of macrophage presence has shown some beneficial effects on the healing process. [16][17][18] Collectively, these data suggest that a more translationally-tenable therapeutic approach to enhancing healing via modulation of the macrophage environment is likely to involve blunting rather than complete attenuation of macrophage recruitment and temporal control of macrophage dynamics. To that end, we leveraged a small molecule antagonist of Ccr2, which blunts circulating monocyte/macrophage recruitment to sites of injury or…”

Section: Introductionmentioning

confidence: 97%

Pharmacological Antagonism of Ccr2+ Cell Recruitment to Facilitate Regenerative Tendon Healing

Smolyak,

Rodenhouse,

Nichols

et al. 2024

Preprint

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Successful tendon healing requires sufficient deposition and remodeling of new extracellular matrix at the site of injury, with this process mediating in part through fibroblast activation via communication with macrophages. Moreover, resolution of healing requires clearance or reversion of activated cells, with chronic interactions with persistent macrophages impairing resolution and facilitating the conversion the conversion to fibrotic healing. As such, modulation of the macrophage environment represents an important translational target to improve the tendon healing process. Circulating monocytes are recruited to sites of tissue injury, including the tendon, via upregulation of cytokines including Ccl2, which facilitates recruitment of Ccr2+ macrophages to the healing tendon. Our prior work has demonstrated that Ccr2-/-can modulate fibroblast activation and myofibroblast differentiation. However, this approach lacked temporal control and resulted in healing impairments. Thus, in the current study we have leveraged a Ccr2 antagonist to blunt macrophage recruitment to the healing tendon in a time-dependent manner. We first tested the effects of Ccr2 antagonism during the acute inflammatory phase and found that this had no effect on the healing process. In contrast, Ccr2 antagonism during the late inflammatory/ early proliferative period resulted in significant improvements in mechanical properties of the healing tendon. Collectively, these data demonstrate the temporally distinct impacts of modulating Ccr2+ cell recruitment and Ccr2 antagonism during tendon healing and highlight the translational potential of transient Ccr2 antagonism to improve the tendon healing process.

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Role of Macrophages During early Achilles Tendon Healing

Cited by 2 publications

References 17 publications

Histology of tendon and enthesis – suitable techniques for specific research questions

Histology of tendon and enthesis – suitable techniques for specific research questions

Pharmacological Antagonism of Ccr2+ Cell Recruitment to Facilitate Regenerative Tendon Healing

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