Role of Macrophage Scavenger Receptors in Hepatic Granuloma Formation in Mice

Hagiwara, Sho Ichiro; Takeya, Motohiro; Suzuki, Hiroshi; Kodama, Tatsuhiko; Laan, Luc J. W. van der; Kraal, Georg; Kitamura, Nobuo; Takahashi, Kiyoshi

doi:10.1016/s0002-9440(10)65317-5

Cited by 22 publications

(14 citation statements)

References 58 publications

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“…Similarly, the SR-A mRNA is transiently down regulated after an in vivo challenge with LPS in mice (461). However, treatment of mice with inflammatory stimuli like BCG or heatkilled Corynebacterium parvum results in increased expression on liver Kupffer cells (181,198,523). Also, the expression of MARCO can be upregulated by inflammatory stimuli; in mice, treatments with BCG, C. parvum, K. pneumoniae, and LPS all resulted in a marked upregulation of MARCO expression in the liver (181,544,545).…”

Section: Scavenger Receptorsmentioning

confidence: 99%

“…However, treatment of mice with inflammatory stimuli like BCG or heatkilled Corynebacterium parvum results in increased expression on liver Kupffer cells (181,198,523). Also, the expression of MARCO can be upregulated by inflammatory stimuli; in mice, treatments with BCG, C. parvum, K. pneumoniae, and LPS all resulted in a marked upregulation of MARCO expression in the liver (181,544,545). Moreover, a similar pattern of high MARCO expression was observed in the livers of two newborns who died of sepsis (108).…”

Section: Scavenger Receptorsmentioning

confidence: 99%

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Receptors, Mediators, and Mechanisms Involved in Bacterial Sepsis and Septic Shock

2003

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Bacterial sepsis and septic shock result from the overproduction of inflammatory mediators as a consequence of the interaction of the immune system with bacteria and bacterial wall constituents in the body. Bacterial cell wall constituents such as lipopolysaccharide, peptidoglycans, and lipoteichoic acid are particularly responsible for the deleterious effects of bacteria. These constituents interact in the body with a large number of proteins and receptors, and this interaction determines the eventual inflammatory effect of the compounds. Within the circulation bacterial constituents interact with proteins such as plasma lipoproteins and lipopolysaccharide binding protein. The interaction of the bacterial constituents with receptors on the surface of mononuclear cells is mainly responsible for the induction of proinflammatory mediators by the bacterial constituents. The role of individual receptors such as the toll-like receptors and CD14 in the induction of proinflammatory cytokines and adhesion molecules is discussed in detail. In addition, the roles of a number of other receptors that bind bacterial compounds such as scavenger receptors and their modulating role in inflammation are described. Finally, the therapies for the treatment of bacterial sepsis and septic shock are discussed in relation to the action of the aforementioned receptors and proteins

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Section: Scavenger Receptorsmentioning

confidence: 99%

Section: Scavenger Receptorsmentioning

confidence: 99%

Receptors, Mediators, and Mechanisms Involved in Bacterial Sepsis and Septic Shock

2003

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“…The samples were subjected to reverse transcriptase reaction using a random primer, and PCR reaction was performed as described elsewhere (Hagiwara et al, 1999). The primer pairs for human ACAT1 and ACAT2 and mouse ACAT2 used in these experiments are summarized in Table 1.…”

Section: Rt-pcrmentioning

confidence: 99%

Acyl-Coenzyme A:Cholesterol Acyltransferase 2 (ACAT2) Is Induced in Monocyte-Derived Macrophages: In Vivo and In Vitro Studies

Sakashita

Miyazaki

Chang

et al. 2003

Laboratory Investigation

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SUMMARY:To test the possibility that acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) may be expressed in human macrophages under pathologic conditions, we employed specific anti-ACAT2 antibodies and found clear ACAT2 signals in lipid-laden as well as lipid-free macrophages under various disease conditions, including atherosclerosis. However, no ACAT2 signal was detectable in macrophages under normal physiologic conditions. Using cultured human macrophages derived from blood-borne monocytes, immunoblot and RT-PCR analyses demonstrated that immature macrophages expressed only ACAT1, but the fully differentiated macrophages expressed both ACAT1 and ACAT2. Furthermore, RT-PCR clearly revealed the presence of both ACAT1 and ACAT2 mRNAs in human atherosclerotic aorta. Double immunohistochemical staining indicated that in human atherosclerotic aorta, all macrophages expressed ACAT1, while approximately 70% to 80% of macrophages also expressed ACAT2. In congenital hyperlipidemic mice, immunohistochemistry and RT-PCR demonstrated that ACAT2 was also present in lipid-laden cells of the atheromatous plaques. Our results suggest that in atherosclerotic plaque, the ability of macrophage foam cell transformation may be augmented by the dual expressions of ACAT1 and ACAT2. Additional immunoblot and RT-PCR experiments showed that the ACAT2 signal was clearly detectable in thioglycollate-elicited exudate mouse macrophages but not in peritoneal resident macrophages. We conclude that under various pathologic conditions, fully differentiated macrophages express ACAT2 in addition to ACAT1. (Lab Invest 2003, 83:1569 -1581.

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“…To detect the GM-CSF mRNA, we used the RT reaction, performed as reported previously, 65,66 with a random primer, nested PCR was done using the following primers. The sequences of outer and inner primers of GM-CSF were as follows: outer primer: sense: 5Ј-TGAGGAGGATGTGGC-TGCA-3Ј, antisense: 5Ј-CAGGCACAAAAGCAGCAGTC-3Ј, size of the amplified product, 488 bp; inner primer: sense: 5Ј-TGTGGTCTACAGCCTCTCAGCAC-3Ј, antisense: 5Ј-CAA-AGGGGATATCAGTCAGAAAGGT-3Ј, size of the amplified product, 368 bp (Table 2).…”

Section: Rt Reaction With Nested Pcrmentioning

confidence: 99%

Role of Granulocyte/Macrophage Colony-Stimulating Factor in Zymocel-Induced Hepatic Granuloma Formation

Wynn

Miyakawa

Miyata

et al. 2001

The American Journal of Pathology

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Role of Macrophage Scavenger Receptors in Hepatic Granuloma Formation in Mice

Cited by 22 publications

References 58 publications

Receptors, Mediators, and Mechanisms Involved in Bacterial Sepsis and Septic Shock

Receptors, Mediators, and Mechanisms Involved in Bacterial Sepsis and Septic Shock

Acyl-Coenzyme A:Cholesterol Acyltransferase 2 (ACAT2) Is Induced in Monocyte-Derived Macrophages: In Vivo and In Vitro Studies

Role of Granulocyte/Macrophage Colony-Stimulating Factor in Zymocel-Induced Hepatic Granuloma Formation

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