Role of M2 macrophages-derived extracellular vesicles in IL-1β-stimulated chondrocyte proliferation and inflammatory responses

Guo, Weiwei; Li, Su; Zhang, Hao; Mi, Zhanhu

doi:10.1007/s10561-022-10016-6

Cited by 3 publications

(4 citation statements)

References 47 publications

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“…14 Only two studies investigated the effects of macrophagederived EVs on OA. 16,29 One study reported that peritoneal macrophage-derived M2-EVs inhibited inflammation and promoted the proliferation of IL-1β-stimulated chondrocytes in vitro. 16 This study showed that M2-EVs enhanced chondrocyte colony formation and viability, increased IL-10 levels, and decreased TNF-α and IL-18 levels.…”

Section: Discussionmentioning

confidence: 99%

“…16,29 One study reported that peritoneal macrophage-derived M2-EVs inhibited inflammation and promoted the proliferation of IL-1β-stimulated chondrocytes in vitro. 16 This study showed that M2-EVs enhanced chondrocyte colony formation and viability, increased IL-10 levels, and decreased TNF-α and IL-18 levels. However, these findings were not validated in vivo, and the repolarization ability of M2-EVs in macrophages was not investigated.…”

Section: Discussionmentioning

confidence: 99%

“…15 Only one study evaluated M2-EVs in OA and found that M2-EVs inhibited inflammation and promoted the proliferation of IL-1β-stimulated chondrocytes in vitro. 16 However, this study did not assess the repolarization ability of M2-EVs on macrophages or verify their findings in vivo. Notably, another study demonstrated the remarkable in situ macrophage-repolarizing capacity of M2-EVs, in which synovial macrophages switched from the M1 to M2 phenotype in a collagen-induced rheumatoid arthritis (RA) mouse model.…”

Section: Introductionmentioning

confidence: 87%

“…Only one study evaluated M2-EVs in OA and found that M2-EVs inhibited inflammation and promoted the proliferation of IL-1β-stimulated chondrocytes in vitro . However, this study did not assess the repolarization ability of M2-EVs on macrophages or verify their findings in vivo.…”

Section: Introductionmentioning

confidence: 99%

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M2 Macrophage-Derived Extracellular Vesicles Encapsulated in Hyaluronic Acid Alleviate Osteoarthritis by Modulating Macrophage Polarization

Yuan,

Yang,

Zheng

et al. 2024

ACS Biomater. Sci. Eng.

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An imbalance between M1 and M2 macrophage polarization is critical in osteoarthritis (OA) development. We investigated the effect of M2 macrophage-derived extracellular vesicles (M2-EVs) to reprogramme macrophages from the M1 to M2 phenotype for OA treatment. M1 macrophages and mouse OA models were treated with M2-EVs. Proteomic analysis was performed to evaluate macrophage polarization in vitro. The OA models were as follows: destabilization of the medial meniscus (DMM) surgery-induced OA and collagenase-induced OA (CIOA). Hyaluronic acid (HA) was used to deliver M2-EVs. M2-EVs decreased macrophage accumulation, repolarized macrophages from the M1 to M2 phenotype, mitigated synovitis, reduced cartilage degradation, alleviated subchondral bone damage, and improved gait abnormalities in the CIOA and DMM models. Moreover, HA increased the retention time of M2-EVs and enhanced the efficiency of M2-EVs in OA treatment. Furthermore, proteomic analysis demonstrated that M2-EVs exhibited a macrophage reprogramming ability similar to IL-4, and the pathways might be the NOD-like receptor (NLR), TNF, NF-κB, and Toll-like receptor (TLR) signaling pathways. M2-EVs reprogrammed macrophages from the M1 to M2 phenotype, which resulted in beneficial effects on cartilage and attenuation of OA severity. In summary, our study indicated that M2-EV-guided reprogramming of macrophages is a promising treatment strategy for OA.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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M2 Macrophage-Derived Extracellular Vesicles Encapsulated in Hyaluronic Acid Alleviate Osteoarthritis by Modulating Macrophage Polarization

Yuan,

Yang,

Zheng

et al. 2024

ACS Biomater. Sci. Eng.

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Genetically engineered loaded extracellular vesicles for drug delivery

Erana-Perez,

Igartua,

Santos-Vizcaino

et al. 2024

Trends in Pharmacological Sciences

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Extracellular vesicles the delivery of drugs through genetic engineering

Ankur,

Priyanka,

Neelesh

et al. 2024

IJPCA

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Researchers from a variety of disciplines are investigating the use of extracellular vesicles (EVs) as delivery vehicles for pharmaceutical solutions. In order to make the most of the therapeutic potential of EVs, various loading strategies have been devised. Despite the widespread application of exogenous methods, endogenous approaches are becoming increasingly preferred instead. Medicinal macro molecules such as proteins and nucleic acids can be loaded with relative ease using this technology that manipulates parental cells through genetic engineering. We examine the most effective ways for EV loading and demonstrate why endogenous is superior. Extracellular vesicles (EVs), which are able to transport pharmaceuticals, have the potential to tackle the problems of biologic therapeutic stability and off-target undesirable effects. In addition, we investigate the most recent findings and applications of this innovative method in order to shed light on the numerous potential therapeutic possibilities in the field of EV-based treatments. The possibility of endogenous loading of EVs with big biological medicines has been brought to light by recent research. In order to optimize the loading of biologic drugs in extracellular vesicles (EVs), researchers have been able to apply cargo selection and loading pathway molecules thanks to advancements in EV biogenesis. It has been established that endogenous EV loading is successful in preclinical in vivo trials, which demonstrates its promise in a variety of therapeutic situations.

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Role of M2 macrophages-derived extracellular vesicles in IL-1β-stimulated chondrocyte proliferation and inflammatory responses

Cited by 3 publications

References 47 publications

M2 Macrophage-Derived Extracellular Vesicles Encapsulated in Hyaluronic Acid Alleviate Osteoarthritis by Modulating Macrophage Polarization

M2 Macrophage-Derived Extracellular Vesicles Encapsulated in Hyaluronic Acid Alleviate Osteoarthritis by Modulating Macrophage Polarization

Genetically engineered loaded extracellular vesicles for drug delivery

Extracellular vesicles the delivery of drugs through genetic engineering

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