Role of LOXs and COX-2 on FAK activation and cell migration induced by linoleic acid in MDA-MB-231 breast cancer cells

Serna-Márquez, Nathalia; Villegas-Comonfort, Sócrates; Galindo-Hernández, Octavio; Navarro-Tito, Napoleón; Millán, Alejandro; Salazar, Eduardo Pérez

doi:10.1007/s13402-012-0114-4

Cited by 34 publications

(40 citation statements)

References 66 publications

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“…Specifically, treatment of MDA-MB-231 cells with 90 μM LA induces FAK activation and migration through a SRC, Gi/Go, COX-2, and LOXs-activity dependent pathway, whereas treatment of nonmalignant mammary epithelial cell line MCF10A with 60 μM LA mediates an EMT-like process. 5,11 In the present study, we demonstrate that treatment with LA increases IR and/or IGF1R expression in the breast cancer cell lines MDA-MB-231 and MCF-7. In contrast, LA does not regulate IR and IGF1R expression in non-tumorigenic MCF12A mammary epithelial cells and it is feasible that LA is only able to induce IR and IGF1R expression in breast cancer cells.…”

Section: Discussionsupporting

confidence: 58%

“…Supporting our proposal, LA induces migration through a LOXs-dependent pathway in MDA-MB-231 cells. 5 It remains to be investigated the specific members of LOXs and the eicosanoids that mediate the increase of IR and IGF1R expression. inhibitor AH7614 (A and B), the LOXs inhibitor NDGA or the SRC inhibitor PP2 (C and D) and stimulated with 90 μM LA.…”

Section: Discussionmentioning

confidence: 99%

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Linoleic acid induces an increased response to insulin in MDA‐MB‐231 breast cancer cells

Rodriguez‐Monterrosas

Diaz-Aragon²,

Cortés‐Reynosa³

et al. 2018

J of Cellular Biochemistry

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Epidemiological studies and animal models suggest a link between high levels of dietary fat intake and an increased risk of developing breast cancer. Hyperinsulinemia is a feature of obesity, diabetes, and metabolic syndrome that is associated with an increased breast cancer risk. Insulin is a hormone involved in metabolic regulation of carbohydrate. However, it is also a growth factor that mediates proliferation and migration. Linoleic acid (LA) is a fatty acid that induces migration and invasion in breast cancer cells. In the present study, we demonstrate, for the first time, that treatment with LA increases IR and IGF1R expression through a Free Fatty Acid Receptor 4 (FFAR4)-, lipooxygenases (LOXs)-, and SRC-dependent pathway in MDA-MB-231 breast cancer cells, and similarly induces an increase of IR expression in MCF-7 breast cancer cells. In addition, insulin induces tyrosine phosphorylation of IR/IGF1R and migration in MDA-MB-231 cells pretreated with LA, whereas it augments the increase in migration in MCF-7 cells pretreated with LA. Pretreatment of MDA-MB-231 cells with LA induces invasion, proliferation, and increase the MMP-9 secretion induced by insulin. In summary, our findings demonstrate that treatment with LA induces a higher response to insulin in breast cancer cells.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Linoleic acid induces an increased response to insulin in MDA‐MB‐231 breast cancer cells

Rodriguez‐Monterrosas

Diaz-Aragon²,

Cortés‐Reynosa³

et al. 2018

J of Cellular Biochemistry

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“…We previously demonstrated that LA induces FAK activation and migration through a concentration-dependent manner in MDA-MB-231 cells, with a maximum of FAK activation and migration at 90 mM LA [20]. In addition, treatment of MCF10A cells with 60 mM LA induces an EMT-like process [11].…”

Section: Discussionmentioning

confidence: 94%

“…Particularly, LA is the major PUFA in the diet of adult population, and it has been estimated to be consumed at 15-20 g per day per person, with a plasma concentration of $275 mM [35][36][37]. In addition, LA induces FAK activation and cell migration in MDA-MB-231 cells, as well as an EMT-like process in MCF10A cells [11,20].…”

Section: Discussionmentioning

confidence: 99%

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Extracellular vesicles from MDA-MB-231 breast cancer cells stimulated with linoleic acid promote an EMT-like process in MCF10A cells

Galindo-Hernández

Serna-Márquez

Castillo-Sánchez

et al. 2014

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Identification and Characterization of Robust Hepatocellular Carcinoma Prognostic Subtypes Based on an Integrative Metabolite‐Protein Interaction Network

et al. 2021

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Metabolite-protein interactions (MPIs) play key roles in cancer metabolism. However, our current knowledge about MPIs in cancers remains limited due to the complexity of cancer cells. Herein, the authors construct an integrative MPI network and propose a MPI network based hepatocellular carcinoma (HCC) subtyping and mechanism exploration workflow. Based on the expressions of hub proteins on the MPI network, two prognosis-distinctive HCC subtypes are identified. Meanwhile, multiple interdependent features of the poor prognostic subtype are observed, including hypoxia, DNA hypermethylation of metabolic pathways, fatty acid accumulation, immune pathway up-regulation, and exhausted T-cell infiltration. Notably, the immune pathway up-regulation is probably induced by accumulated unsaturated fatty acids which are predicted to interact with multiple immune regulators like SRC and TGFB1. Moreover, based on tumor microenvironment compositions, the poor prognostic subtype is further divided into two sub-populations showing remarkable differences in metabolism. The subtyping shows a strong consistency across multiple HCC cohorts including early-stage HCC. Overall, the authors redefine robust HCC prognosis subtypes and identify potential MPIs linking metabolism to immune regulations, thus promoting understanding and clinical applications about HCC metabolism heterogeneity.

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Role of LOXs and COX-2 on FAK activation and cell migration induced by linoleic acid in MDA-MB-231 breast cancer cells

Abstract: In summary, our findings show that LA induces FAK activation and cell migration in MDA-MB-231 breast cancer cells.

Cited by 34 publications

References 66 publications

Linoleic acid induces an increased response to insulin in MDA‐MB‐231 breast cancer cells

Linoleic acid induces an increased response to insulin in MDA‐MB‐231 breast cancer cells

Extracellular vesicles from MDA-MB-231 breast cancer cells stimulated with linoleic acid promote an EMT-like process in MCF10A cells

Identification and Characterization of Robust Hepatocellular Carcinoma Prognostic Subtypes Based on an Integrative Metabolite‐Protein Interaction Network

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