Role of lipoproteins in growth of human adult arterial endothelial and smooth muscle cells in low lipoprotein‐deficient serum

Abstract

Recently improved culture conditions for human adult arterial endothelial and smooth muscle cells from a wide variety of donors have been used to study the effects of lipoproteins on proliferation of both cell types in low serum culture medium. Optimal growth of endothelial and smooth muscle cells in an optimal nutrient medium (MCDB 107) containing epidermal growth factor, a partially purified fraction from bovine brain, and 1% (v/v) lipoprotein-deficient serum was dependent on either high- or low-density lipoprotein. High- and low-density lipoprotein stimulated cell growth by three- and five-fold, respectively, over a 6-day period. Optimal stimulation of both endothelial and smooth muscle cell growth occurred between 20 and 60 micrograms/ml of high- and low-density lipoproteins, respectively. No correlation between the activation of 3-hydroxyl-3-methylglutaryl coenzyme. A reductase activity and lipoprotein-stimulated cell proliferation was observed. Lipid-free total apolipoproteins or apolipoprotein C peptides from high-density lipoprotein were partially effective and together with oleic acid effectively replaced native high-density lipoprotein for the support of endothelial cell growth. In contrast, apolipoproteins or apolipoprotein C peptides from high-density lipoprotein alone or with oleic acid had no effect on smooth muscle cell proliferation. The results suggest a functional role of high- and low-density lipoproteins and apolipoproteins in the proliferation of human adult endothelial and smooth muscle cells.

Herpes simplex virus type-1 infection upregulates cellular promoters and telomerase activity in both tumor and nontumor human cells

“…Normal endothelial cells (HUVEC) were isolated from human umbilical cord veins as described previously. 77 Cells were cultured in medium MCDB 107 (JRH Biosciences, Lenexa, KS, USA) supplemented with 2% FCS and a fibroblast growth factor-enriched fraction of porcine brain extract (1 mg/ml).…”

Mitogenic Effect of Lipoproteins on Human Vascular Smooth Muscle Cells: The Impact of Hydrolysis by gr II A Phospholipase A2

“…Whereas only a minimal effect was reported (Libby et al 1985), others found that LDL has a definite mitogenic effect (Fang et al, 1997;Locher et al, 1992). The effect was more pronounced when VSMC were cultured in low-serum medium (Chen et al, 1986) or when LDL was oxidized (Heery et al, 1995). In our system, LDL induced pronounced mitogenic activity that was further enhanced by extrinsic sPLA 2 .…”

“…The impact of LDL on mitogenic activity of VSMC was found to vary, depending on experimental conditions (Chen et al, 1986;Fang et al, 1997;Heery et al, 1995;Leitinger et al, 1999;Libby et al, 1985;Locher et al, 1992;Scott-Burden et al, 1989). Whereas only a minimal effect was reported (Libby et al 1985), others found that LDL has a definite mitogenic effect (Fang et al, 1997;Locher et al, 1992).…”

Lipoprotein(a) stimulates the proliferation of cultured human arterial smooth muscle cells through two pathways

“…Lipoprotein-deficient serum (LPDS/was prepared from FBS by the method of Chen et al [15]. Briefly, FBS was adjusted to a density of 1.21 g/ml with solid potassium bromide (KBr) and centrifuged at 140,000 x g for 48 hours.…”