Role of lipid membrane–nucleic acid interactions, DNA–membrane contacts and metal (II) cations in origination of initial cells and in evolution of prokaryotes to eukaryotes

Жданов, Р. И.; Kuvichkin, Vasily; Shmyrina, A. S.; Jdanov, A. R.; Твердислов, В. А.

doi:10.1016/s1567-5394(02)00134-2

Cited by 5 publications

(4 citation statements)

References 26 publications

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“…Lipofection reagents efficiently transfer siRNAs, microRNAs, or other oligonucleotides into postmitotic neurons (with up to 83% efficiency in primary rat hippocampal neurons) (Tonges et al, 2006). Finally, there is great interest in lipid-based DNA delivery for gene therapy, as this method has a lower risk of causing mutations and immune responses than virus-based delivery (Zhdanov et al, 2002).…”

Section: Lipofectionmentioning

confidence: 99%

Transfection Techniques for Neuronal Cells: Table 1.

Karra

Dahm²

2010

J. Neurosci.

176

165

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Editor's Note: Toolboxes are intended to describe and evaluate methods that are becoming widely relevant to the neuroscience community or to provide a critical analysis of established techniques. For more information, see http://www.jneurosci.org/misc/ ifa_minireviews.dtl. Transfection Techniques for Neuronal CellsDaniela Karra 1 and Ralf Dahm 2,3 1 Institute for Neuropathology, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany, 2 Department of Biology, University of Padua, I-35121 Padua, Italy, and 3 Spanish National Cancer Research Centre (CNIO), E-28029 Madrid, Spain IntroductionThe transfection of nucleic acids into cells is crucial for the study of many aspects of neuronal cell biology. These include investigating gene and protein function by knocking down target proteins via RNA interference (RNAi) or microRNAs, expressing tagged proteins to track their subcellular localization, behavior, and turnover; and expressing mutant versions of proteins to study the functions of specific domains or mimic disease conditions. Moreover, reporter proteins can be used to detect intracellular ion concentrations or levels of gene expression.Despite efforts to optimize transfection techniques and protocols for neurons, no method has yet been developed that is suitable for all applications. Instead, the various established methods have their own advantages and drawbacks concerning transfection efficiency, expression levels, cell survival, and viability. Other considerations are the ease of use, reproducibility, cost, and applicability to a given experiment. Researchers therefore often face a bewildering roster of possibilities, making it difficult to decide which approach to take.In this review we provide a brief overview of methods used to transfect mam-

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Section: Lipofectionmentioning

confidence: 99%

Transfection Techniques for Neuronal Cells: Table 1.

Karra

Dahm²

2010

J. Neurosci.

176

165

View full text Add to dashboard Cite

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“…Several studies showed binding and disruption of phospholipid bilayers by supramolecular RNA complexes and suggested that specific RNA binding to phospholipids can modulate RNA–membrane interactions (18–20). Similarly, the role of lipid membrane–RNA interactions in cell evolution of prokaryotes and eukaryotes has been investigated (21). Even though much of lipid–RNA interactions are associated with phospholipids bilayers, structural characterization of cationic lipid–RNA complexes has major biological importance due to the applications of cationic lipids in siRNA and miRNA delivery in vivo (22–26).…”

Section: Introductionmentioning

confidence: 99%

Structural characterization of cationic lipid-tRNA complexes

Marty

N’soukpoé‐Kossi

Charbonneau

et al. 2009

Nucleic Acids Research

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Despite considerable interest and investigations on cationic lipid–DNA complexes, reports on lipid–RNA interaction are very limited. In contrast to lipid–DNA complexes where lipid binding induces partial B to A and B to C conformational changes, lipid–tRNA complexation preserves tRNA folded state. This study is the first attempt to investigate the binding of cationic lipid with transfer RNA and the effect of lipid complexation on tRNA aggregation and condensation. We examine the interaction of tRNA with cholesterol (Chol), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), dioctadecyldimethylammoniumbromide (DDAB) and dioleoylphosphatidylethanolamine (DOPE), at physiological condition, using constant tRNA concentration and various lipid contents. FTIR, UV-visible, CD spectroscopic methods and atomic force microscopy (AFM) were used to analyze lipid binding site, the binding constant and the effects of lipid interaction on tRNA stability, conformation and condensation. Structural analysis showed lipid–tRNA interactions with G–C and A–U base pairs as well as the backbone phosphate group with overall binding constants of KChol = 5.94 (± 0.8) × 104 M–1, KDDAB = 8.33 (± 0.90) × 105 M–1, KDOTAP = 1.05 (± 0.30) × 105 M–1 and KDOPE = 2.75 (± 0.50) × 104 M–1. The order of stability of lipid–tRNA complexation is DDAB > DOTAP > Chol > DOPE. Hydrophobic interactions between lipid aliphatic tails and tRNA were observed. RNA remains in A-family structure, while biopolymer aggregation and condensation occurred at high lipid concentrations.

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“…Additionally, liposomes do not induce strong toxicity and are highly reproducible when used for transfecting various neurons 84,91 . Compared to viral delivery methods, there is also a lower risk of mutation and immune-related issues 92 . Expanding from the success of in vitro neuronal cell transfection, lipid-based vectors have also been widely used for in vivo studies, as highlighted in the subsequent section.…”

Section: Lipofectionmentioning

confidence: 99%