Role of Lipid Composition, Physicochemical Interactions, and Membrane Mechanics in the Molecular Actions of Microbial Cyclic Lipopeptides

Balleza, Daniel; Alessandrini, Andrea; García, Miguel Juan Beltrán

doi:10.1007/s00232-019-00067-4

Cited by 62 publications

(44 citation statements)

References 206 publications

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“…Most natural antimicrobial peptides primarily act on the bacterial cell membrane [26]. The outer cell membrane provides an additional layer of protection for the organism without affecting the exchange of substances required for life.…”

Section: Resultsmentioning

confidence: 99%

Bioactivity and Bactericidal Mechanism of Histidine-Rich β-Hairpin Peptide Against Gram-Negative Bacteria

Dong

Wang

Zhang

et al. 2019

IJMS

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Antibacterial peptides (APMs) are a new type of antibacterial substance. The relationship between their structure and function remains indistinct; in particular, there is a lack of a definitive and fixed template for designing new antimicrobial peptides. Previous studies have shown that porcine Protegrin-1 (PG-1) exhibits considerable antimicrobial activity and cytotoxicity. In this study, to reduce cytotoxicity and increase cell selectivity, we designed histidine-rich peptides based on the sequence template RR(XY)2XDPGX(YX)2RR-NH2, where X represents I, W, V, and F. The results showed that the peptides form more β-hairpin structures in a lipid-rich environment that mimics cell membranes. Among them, the antimicrobial peptide HV2 showed strong antibacterial activity against Gram-negative strains and almost no toxicity to normal cells. The results of our analysis of its antibacterial mechanism showed that peptide HV2 acts on the bacterial cell membrane to increase its permeability, resulting in cell membrane disruption and death. Furthermore, peptide HV2 inhibited bacterial movement in a concentration-dependent manner and had a more robust anti-inflammatory effect by inhibiting the production of TNF-α. In summary, peptide HV2 exhibits high bactericidal activity and cell selectivity, making it a promising candidate for future use as an antibiotic.

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Section: Resultsmentioning

confidence: 99%

Bioactivity and Bactericidal Mechanism of Histidine-Rich β-Hairpin Peptide Against Gram-Negative Bacteria

Dong

Wang

Zhang

et al. 2019

IJMS

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“…In the literature, evidence of the dependence of FE activity on the presence of negatively charged species in the membrane can be found 33 . To estimate the contribution of lipid charge to the channel formation ability of FE, we investigated the effects of replacing the negatively charged POPG with uncharged GMO.…”

Section: Resultsmentioning

confidence: 99%

Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

Zakharova

Efimova

Malev

et al. 2019

Sci Rep

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The one-sided addition of fengycin (FE) to planar lipid bilayers mimicking target fungal cell membranes up to 0.1 to 0.5 μM in the membrane bathing solution leads to the formation of well-defined and well-reproducible single-ion channels of various conductances in the picosiemens range. FE channels were characterized by asymmetric conductance-voltage characteristic. Membranes treated with FE showed nonideal cationic selectivity in potassium chloride bathing solutions. The membrane conductance induced by FE increased with the second power of the lipopeptide aqueous concentration, suggesting that at least FE dimers are involved in the formation of conductive subunits. The pore formation ability of FE was not distinctly affected by the molecular shape of membrane lipids but strongly depended on the presence of negatively charged species in the bilayer. FE channels were characterized by weakly pronounced voltage gating. Small molecules known to modify the transmembrane distribution of electrical potential and the lateral pressure profile were used to modulate the channel-forming activity of FE. The observed effects of membrane modifiers were attributed to changes in lipid packing and lipopeptide oligomerization in the membrane.

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“…In LPQ strains multiple uncharacterized LuxR-type regulators are present (Figures 3 and 5) and it seems possible that some of them also respond to specific secondary metabolites from plants (Subramoni et al 2011). It also appears that the phytotoxic and antimicrobial activities of CLPs are linked to their ability to interact with membranes, where the nature of the sphingolipids and/or the sterols is of prime importance to determine whether or not a target is sensitive (Balleza et al 2019). Membrane composition greatly varies according to the stage of development and the different part of the plant (roots, leaves, fruits … .…”

Section: Discussionmentioning

confidence: 99%

Lipopeptide families at the interface between pathogenic and beneficialPseudomonas-plant interactions

Girard

Höfte

Mot

2020

Critical Reviews in Microbiology

106

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Lipopeptides (LPs) are a prominent class of molecules among the steadily growing spectrum of specialized metabolites retrieved from Pseudomonas, in particular soil-dwelling and plant-associated isolates. Among the multiple LP families, pioneering research focussed on phytotoxic and antimicrobial cyclic lipopeptides (CLPs) of the ubiquitous plant pathogen Pseudomonas syringae (syringomycin and syringopeptin). Their non-ribosomal peptide synthetases (NRPSs) are embedded in biosynthetic gene clusters (BGCs) that are tightly co-clustered on a pathogenicity island. Other members of the P. syringae group (Pseudomonas cichorii) and some species of the Pseudomonas asplenii group and Pseudomonas fluorescens complex have adopted these biosynthetic strategies to co-produce their own mycin and peptin variants, in some strains supplemented with an analogue of the P. syringae linear LP (LLP), syringafactin. This capacity is not confined to phytopathogens but also occurs in some biocontrol strains, which indicates that these LP families not solely function as general virulence factors. We address this issue by scrutinizing the structural diversity and bioactivities of LPs from the mycin, peptin, and factin families in a phylogenetic and evolutionary perspective. BGC functional organization (including associated regulatory and transport genes) and NRPS modular architectures in known and candidate LP producers were assessed by genome mining.

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Role of Lipid Composition, Physicochemical Interactions, and Membrane Mechanics in the Molecular Actions of Microbial Cyclic Lipopeptides

Cited by 62 publications

References 206 publications

Bioactivity and Bactericidal Mechanism of Histidine-Rich β-Hairpin Peptide Against Gram-Negative Bacteria

Bioactivity and Bactericidal Mechanism of Histidine-Rich β-Hairpin Peptide Against Gram-Negative Bacteria

Fengycin induces ion channels in lipid bilayers mimicking target fungal cell membranes

Lipopeptide families at the interface between pathogenic and beneficialPseudomonas-plant interactions

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