Role of Lipid Composition in the Interaction and Activity of the Antimicrobial Compound Fengycin with Complex Membrane Models

Mantil, Elisabeth; Buznytska, Iryna; Daly, Grace; Ianoul, Anatoli; Avis, Tyler J.

doi:10.1007/s00232-019-00100-6

Cited by 14 publications

(12 citation statements)

References 39 publications

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“…There is a lack of studies using leakage assays to investigate the interactions occurring at the bio-nano interface, involving protein-based nanodelivery systems and giant unilamellar vesicles. Existing information on organic compounds, such as antibiotic peptides fengycins [ 20 ], maculatin, citropin, and aurein [ 21 ], showed the disruption of liposomes as a measure of their antimicrobial properties. Liposome leakage data have also been reported for organic nanoparticles, such as lysozyme functionalized single-walled carbon nanotube, amyloid fibril in nanometer-order and lysozyme aggregates [ 12 ], semihydrophobic nanoparticles of polystyrene [ 19 ], and nanoscale protein aggregates [ 22 ], focusing on cytotoxicity of the nanomaterials.…”

Section: Resultsmentioning

confidence: 99%

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Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles

2022

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Curcumin-loaded native and succinylated pea protein nanoparticles, as well as zwitterionic giant unilamellar vesicles were used in this study as model bioactive compound loaded-nanoparticles and biomembranes, respectively, to assess bio-nano interactions. Curcumin-loaded native protein-chitosan and succinylated protein-chitosan complexes, as well as native protein-chitosan and succinylated protein-chitosan hollow, induced leakage of the calcein encapsulated in the giant unilamellar vesicles. The leakage was more pronounced with hollow protein-chitosan complexes. However, curcumin-loaded native protein and curcumin-loaded succinylated protein nanoparticles induced calcein fluorescence quenching. Dynamic light scattering measurements showed that the interaction of curcumin-loaded native protein, curcumin-loaded succinylated protein, native protein-chitosan, and succinylated protein-chitosan complexes with the giant unilamellar vesicles caused a major reduction in the size of the lipid vesicles. Confocal and widefield fluorescence microscopy showed rupturing of the unilamellar vesicles after treatment with native pea protein-chitosan and succinylated pea protein-chitosan complexes. The nature of interaction between the curcumin-loaded protein nanoparticles and the biomembranes, at the bio-nano interface, is influenced by the encapsulated curcumin. Findings from this study showed that, as the protein plays a crucial role in stabilizing the bioactive compound from chemical and photodegradation, the encapsulated nutraceutical stabilizes the protein nanoparticle to reduce its interaction with biomembranes.

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Section: Resultsmentioning

confidence: 99%

“…This is supported by results from confocal ( Figure 4 h) and widefield fluorescence microscopy ( Figure S1i ). Triton X-100 is a well-known surfactant that causes almost 100% leakage or disruption of liposome vesicles; thus, it is commonly used to estimate the percentage leakage of fluorophore from loaded vesicles ( Section 3.3.1 ) [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles

2022

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“…The micellization process of these amphiphilic molecules is dependent on the chemical structure (chemical composition, length, and isomery of the fatty acid chain) and on environmental parameters of the study (buffer, pH, and temperature) ( Deleu et al, 2013 ). In the scientific literature, CMC of the different LPPs was measured in different aqueous solutions (water or saline buffer) and was from 10 mg·L −1 for surfactin in pH 8.5 in 5 mM of Tris–HCl or in 0.1 M of NaHCO 3 buffer ( Thimon et al, 1992 ; Abdel-Mawgoud et al, 2008 ) to 25 mg·L −1 in water ( Cooper et al, 1981 ), from 39 mg·L −1 in 0.1 M of NaHCO 3 ( Thimon et al, 1992 ) to 44 mg·L −1 for mycosubtilin in KCl 0.1 M ( Maget-Dana et al, 1992 ) and from 2 mg·L −1 ( Eeman et al, 2014 ) to 4 mg·L −1 for fengycin (plipastatin) in PBS buffer ( Mantil et al, 2019 ). A slight impact can be observed on the surface tension profile of the surfactin with a surface tension approximately 10% higher in the surfactin samples, which has undergone a spray-drying step, from the concentration of 10 mg·L −1 .…”

Section: Resultsmentioning

confidence: 99%

Impact of the Purification Process on the Spray-Drying Performances of the Three Families of Lipopeptide Biosurfactant Produced by Bacillus subtilis

Vassaux

Rannou

Peers³

et al. 2021

Front. Bioeng. Biotechnol.

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Lipopeptides produced by Bacillus subtilis display many activities (surfactant, antimicrobial, and antitumoral), which make them interesting compounds with a wide range of applications. During the past years, several processes have been developed to enable their production and purification with suitable yield and purity. The already implemented processes mainly end with a critical drying step, which is currently achieved by freeze-drying. In this study, the possibility to replace this freeze-drying step with a spray-drying one, more suited to industrial applications, was analyzed. After evaluating their thermal resistance, we have developed a spray-drying methodology applicable for the three lipopeptides families produced by B. subtilis, i.e., surfactin, mycosubtilin (iturin family), and plipastatin (fengycin family). For each lipopeptide, the spray-drying procedure was applied at three steps of the purification process by ultrafiltration (supernatant, diafiltered solution, and pre-purified fraction). The analysis of the activities of each spray-dried lipopeptide showed that this drying method is not decreasing its antimicrobial and biosurfactant properties. The methodology developed in this study enabled for the first time the spray-drying of surfactin, without adjuvants’ addition and regardless of the purification step considered. In the case of fengycin and mycosubtilin, only diafiltered solution and purified fraction could be successfully spray-dried without the addition of adjuvant. Maltodextrin addition was also investigated as the solution for the direct drying of supernatant. As expected, the performances of the spray-drying step and the purity of the powder obtained are highly related to the purification step at which the product was dried. Interestingly, the impact of mycosubtilin concentration on spray-drying yield was also evidenced.

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“…Those lipid organization modifications could influence the conformation and/or function of membrane proteins, notably at the level of the lipid domains present in the bacterial IM. However, it has also been recently demonstrated that lipid domains can be formed in the OM, which may have properties and functions different from what is observed in the IM [69]. Additional studies are needed to further explore this hypothesis.…”

Section: General Discussion and Conclusionmentioning

confidence: 98%

“…Then, one can wonder how these specific interactions could be related to the biological effects of 13-HPOT. It is well known that such interactions can influence the membrane organization, the distribution and localization of certain proteins leading notably to the activation of signalling cascade reactions [26,65,69].…”

Section: Involvement Of Specific Lipids In Bpm − 13-hpot Interactionsmentioning

confidence: 99%

Linolenic fatty acid hydroperoxide acts as biocide on plant pathogenic bacteria: Biophysical investigation of the mode of action

Deboever

Lins

Ongena

et al. 2020

Bioorganic Chemistry

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Fatty acid hydroperoxides (HPO) are free phyto-oxylipins known for their crucial role as signalling molecules during plant defense mechanisms. They were also demonstrated to have direct biocidal activities against plant pathogens including gram negative bacteria. In the present work, the biocidal effect of one linolenic fatty acid hydroperoxide, namely 13-HPOT has been investigated on three plant pathogen gram negative bacteria: Pectobacterium carotovorum, Pseudomonas syringae and Xanthomonas translucens. We showed that 13-HPOT has a strong dose response effect on those phytopathogens. In a second part, the molecular mechanism behind the antibacterial effect of 13-HPOT was investigated at a molecular level using an integrative biophysical approach combining in vitro and in silico methods. Since other antimicrobial amphiphilic molecules have been shown to target the lipid membrane of the organisms they act on, we focused our study on the interaction of 13-HPOT with biomimetic membranes. In a first step, we hypothesized that the inner membrane of the bacteria was the main site of action of 13-HPOT and hence we used lipids representative of this membrane to form our models. Our results indicated that 13-HPOT can interact with the lipid representative of the inner bacterial plasma membrane. A strong membrane insertion is suggested but no major permeabilization of the membrane is observed. Phosphatidylethanolamine (PE) and cardiolipin (CL), present in the bacterial plasma membrane, appear to play important roles in this interaction. We suggest that the mode of action of 13-HPOT should involve either subtle changes in membrane properties, such as its lateral organization and distribution, and/or interactions with membrane proteins.

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Role of Lipid Composition in the Interaction and Activity of the Antimicrobial Compound Fengycin with Complex Membrane Models

Cited by 14 publications

References 39 publications

Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles

Curcumin–Induced Stabilization of Protein–Based Nano-Delivery Vehicles Reduces Disruption of Zwitterionic Giant Unilamellar Vesicles

Impact of the Purification Process on the Spray-Drying Performances of the Three Families of Lipopeptide Biosurfactant Produced by Bacillus subtilis

Linolenic fatty acid hydroperoxide acts as biocide on plant pathogenic bacteria: Biophysical investigation of the mode of action

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