Role of light emitting diode (LED) wavelengths on increase of protein productivity and free amino acid profile of Spirulina sp. cultures

Prates, Denise da Fontoura; Duarte, Jessica Hartwig; Vendruscolo, Raquel Guidetti; Wagner, Róger; Ballus, Cristiano Augusto; Oliveira, Wellington da Silva; Godoy, Helena Teixeira; Barcia, Milene Teixeira; Morais, Michele Greque de; Radmann, Elisângela Martha; Costa, Jorge Alberto Vieira

doi:10.1016/j.biortech.2020.123184

Cited by 33 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Spirulina sp. LEB 18, low-energy red light positively contributed to the formation of non-essential amino acids [29], which is in line with our results, especially for serine, aspartic acid and glutamic acid (data not shown). In Phaeodactylum tricornutum, Jungandreas et al [30] concluded that switching between red and blue light wavelengths, which caused vast metabolic reorganisation, was probably related to the engagement of different pathways for carbon partitioning [30].…”

Section: Protein and Amino Acid Dynamicssupporting

confidence: 91%

A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths

Sui

Mazzucchi

Acharya

et al. 2021

Foods

View full text Add to dashboard Cite

Strains of Dunaliella salina microalgae are of considerable research and industrial interest because they hyper-accumulate β-carotene as well as produce high-quality protein. To explore the co-production of valuable compounds in D. salina, this study compared the production of β-carotene, phytoene and amino acids in two strains cultivated under white, red or blue light until no further nitrogen was available. D. salina DF15 (CCAP 19/41 (PLY DF15)) produced more than 12% β-carotene (ash-free dry weight (AFDW) basis), and red light triggered the production of 9-cis β-carotene at a 9-cis/all-trans β-carotene ratio of 1.5. Phytoene production was also evident in D. salina DF15 under all conditions, particularly under blue light. However, the profile of essential amino acids (EAAs) and calculation of the essential amino acid index (EAAI) was less than ideal in terms of protein quality, for both strains. Umami compounds, quantified as monosodium glutamate (MSG) equivalents, indicated a higher equivalent umami concentration (EUC) in D. salina DF15 under red light (3.2 g MSG/100 g AFDW) than in D. salina CCAP19/30. Overall, D. salina DF15 demonstrates valuable traits for further exploration and product optimisation.

show abstract

Section: Protein and Amino Acid Dynamicssupporting

confidence: 91%

A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths

Sui

Mazzucchi

Acharya

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…The concentration of essential and non-essential free amino acids in Spirulina sp. LEB 18 was higher when cultivated under green and blue light, while red light positively contributed to the formation of non-essential amino acids [ 15 ]. When a shifting light strategy from red light to blue light was provided to Phaeodactylum tricornutum , the pool of amino acid content was elevated within 30 min and then decreased after 24 h; when the strategy was from blue light to red light, all amino acid contents quickly decreased after only 15 min [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…Apart from nutrient levels, higher light intensity seems to favour the production of most EAAs except for threonine in D. salina CCAP 19/18, yet delivers a different EAA profile compared with nitrogen limitation [11]. Light-emitting diodes (LEDs) of different wavelengths offer opportunities for delivering efficient, durable, reliable, economic and controllable light in the cultivation of microalgae, and their use has resulted in increased production of biomass and biomolecules [14,15]. In D. salina, different light wavelengths have been studied regarding their effects on carotenoids' synthesis [5,[16][17][18][19][20].…”

Section: Microalgal Strain and Cultivation Methodsmentioning

confidence: 99%

Effect of Light Intensity and Wavelength on Biomass Growth and Protein and Amino Acid Composition of Dunaliella salina

Sui

Harvey

2021

Foods

View full text Add to dashboard Cite

Dunaliella salina is a halotolerant, photoautotrophic marine microalga and one of the richest sources of natural carotenoids but also shows potential as a novel food source with high protein quality. This study sought to optimise the production of biomass, protein and amino acids from D. salina, alongside carotenoids using a two-stage cultivation approach based on the use of light of different intensities and quality, i.e., white, red and blue LED light. In stage 1, four white LED light intensities were tested. In stage 2, the same four light intensities from either blue or red LEDs were applied once exponential growth ceased and cells reached the stationary phase under white LED light in stage 1. Remarkably, both biomass concentration and biomass productivity showed a 1.3–1.7-fold increase in stage 2, without medium replenishment, while protein concentration and protein productivity showed an ~1.1-fold increase. The amino acid content and amino acid index remained unchanged from stage 1 to stage 2, and minimum difference was found across different light intensities. Overall, D. salina delivered so-called high protein quality, with an essential amino acid index (EAAI) of 0.99, and red light, which has previously been shown to increase carotenoid production, boosted further biomass production over and above white light, at all light intensities tested.

show abstract

“…Culture media or light availability can affect protein productivity and amino acid composition (da Fontoura Prates et al. 2020). When used for food or feed, not only the protein content but also the amino acid composition of the proteins is relevant.…”

Section: Resultsmentioning

confidence: 99%

OPTIMIZATION OF A NEW CULTURE MEDIUM FOR THE LARGE‐SCALE PRODUCTION OF PROTEIN‐RICH ARTHROSPIRA PLATENSIS (OSCILLATORIALES, CYANOPHYCEAE)

Gómez-Serrano

Guzmán-Carrasco

Lafarga

et al. 2020

Journal of Phycology

View full text Add to dashboard Cite

Our aim was to develop a novel medium for the large‐scale production of protein‐rich Arthrospira with potential applications as a biofertilizer. The novel culture medium, termed as FM‐II, was formulated using low‐cost commercial chemicals and specifically designed to improve protein production. Both Arthrospira platensis and Arthrospira maxima were produced using FM‐II and Arnon medium, which was used as a control. Photosynthetic status of the cells, which was checked by measuring chlorophyll fluorescence, biomass dry weight and protein content, was assessed daily. Arthrospira platensis had higher biomass and protein productivities than A. maxima when cultured in both control and FM‐II media. Incorporation of varied micronutrients into FM‐II formulation did not improve biomass productivity. Maximum biomass dry weight in FM‐II and control medium was 2.9 and 2.5 g · L−1, respectively. Total protein content of the biomass ranged between 55% and 65%, suggesting potential for being used in the development of high‐value agricultural products. As some nutrients were discarded unused, the initial content of phosphates and bicarbonates was reduced by 75% and 50%, respectively, without affecting the process productivity. Results reported herein could promote the production and utilization of Arthrospira platensis by significantly reducing productions costs and therefore increasing the feasibility of the process.

show abstract

Role of light emitting diode (LED) wavelengths on increase of protein productivity and free amino acid profile of Spirulina sp. cultures

Cited by 33 publications

References 20 publications

A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths

A Comparison of β-Carotene, Phytoene and Amino Acids Production in Dunaliella salina DF 15 (CCAP 19/41) and Dunaliella salina CCAP 19/30 Using Different Light Wavelengths

Effect of Light Intensity and Wavelength on Biomass Growth and Protein and Amino Acid Composition of Dunaliella salina

OPTIMIZATION OF A NEW CULTURE MEDIUM FOR THE LARGE‐SCALE PRODUCTION OF PROTEIN‐RICH ARTHROSPIRA PLATENSIS (OSCILLATORIALES, CYANOPHYCEAE)

Contact Info

Product

Resources

About