Role of La Crosse virus glycoproteins in attachment of virus to host cells

Ludwig, George V.; Israel, Barbara A.; Christensen, Bruce M.; Yuill, Thomas M.; Schultz, Kevin T.

doi:10.1016/0042-6822(91)90889-j

Cited by 77 publications

(43 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As noted above, these results resemble observations on the differential roles of LACV G N and G C glycoproteins in viral neutralization and protection. A soluble version of the G C La Crosse glycoprotein is sufficient to block virus infection in mammalian cells, while antibodies against G N neutralize infection in a mosquito cell line but not in a vertebrate cell line (31). The mechanisms that account for the differential inhibition of CCHFV infection are not currently clear.…”

Section: Discussionmentioning

confidence: 98%

Cellular Localization and Antigenic Characterization of Crimean-Congo Hemorrhagic Fever Virus Glycoproteins

Bertolotti-Ciarlet

Smith

Strecker

et al. 2005

J Virol

144

202

View full text Add to dashboard Cite

Crimean-Congo hemorrhagic fever virus (CCHFV), a member of the genus Nairovirus of the family Bunyaviridae, causes severe disease with high rates of mortality in humans. The CCHFV M RNA segment encodes the virus glycoproteins GN and GC. To understand the processing and intracellular localization of the CCHFV glycoproteins as well as their neutralization and protection determinants, we produced and characterized monoclonal antibodies (MAbs) specific for both GN and GC. Using these MAbs, we found that GN predominantly colocalized with a Golgi marker when expressed alone or with GC, while GC was transported to the Golgi apparatus only in the presence of GN. Both proteins remained endo-β-N-acetylglucosaminidase H sensitive, indicating that the CCHFV glycoproteins are most likely targeted to the cis Golgi apparatus. Golgi targeting information partly resides within the GN ectodomain, because a soluble version of GN lacking its transmembrane and cytoplasmic domains also localized to the Golgi apparatus. Coexpression of soluble versions of GN and GC also resulted in localization of soluble GC to the Golgi apparatus, indicating that the ectodomains of these proteins are sufficient for the interactions needed for Golgi targeting. Finally, the mucin-like and P35 domains, located at the N terminus of the GN precursor protein and removed posttranslationally by endoproteolysis, were required for Golgi targeting of GN when it was expressed alone but were dispensable when GC was coexpressed. In neutralization assays on SW-13 cells, MAbs to GC, but not to GN, prevented CCHFV infection. However, only a subset of GC MAbs protected mice in passive-immunization experiments, while some nonneutralizing GN MAbs efficiently protected animals from a lethal CCHFV challenge. Thus, neutralization of CCHFV likely depends not only on the properties of the antibody, but on host cell factors as well. In addition, nonneutralizing antibody-dependent mechanisms, such as antibody-dependent cell-mediated cytotoxicity, may be involved in the in vivo protection seen with the MAbs to GC

show abstract

Section: Discussionmentioning

confidence: 98%

Cellular Localization and Antigenic Characterization of Crimean-Congo Hemorrhagic Fever Virus Glycoproteins

Bertolotti-Ciarlet

Smith

Strecker

et al. 2005

J Virol

144

202

View full text Add to dashboard Cite

show abstract

“…The family Bunyaviridae is made up mostly of animal viruses transmitted by arthropods, such as mosquitoes and ticks (15). Several bunyaviruses have been shown to infect and replicate in their insect vectors (6,15,19,29). Tospoviruses, the only plant-infecting members of the family Bunyaviridae, also infect their insect vectors, including Frankliniella occidentalis Pergande (Thysanoptera: Thripidae), the western flower thrips (WFT).…”

mentioning

confidence: 99%

The Plant Virus Tomato Spotted Wilt Tospovirus Activates the Immune System of Its Main Insect Vector, Frankliniella occidentalis

Medeiros¹,

Resende

Ávila

2004

J Virol

View full text Add to dashboard Cite

“…The absence of an efficient system for reverse genetics for any of the members of this family has severely limited the elucidation of the primary elements of this relationship. It is only known that determinants of midgut infection and transmission of La Crosse virus (Orthobunyavirus: Bunyaviridae) in the mosquito vector cosegregate with the medium (M) RNA and that proteolytic cleavage of G C is required to infect the midgut epithelium of the mosquito (3)(4)(5)(6). In the case of Tomato spotted wilt virus (TSWV) (Tospovirus: Bunyaviridae), putative thrips receptors have been identified, but there is no specific information regarding viral determinants of the virus-thrips interaction (7)(8)(9).…”

mentioning

confidence: 99%

Viral genetic determinants for thrips transmission of Tomato spotted wilt virus

Sin

McNulty²,

Kennedy³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

121

View full text Add to dashboard Cite

Tomato spotted wilt virus (TSWV) is transmitted exclusively by thrips in nature. A reassortment-based viral genetic system was used to map transmissibility by thrips to the medium (M) RNA of TSWV. To locate determinants of thrips transmission in the M RNA, 30 single-lesion isolates (SLIs) were generated from a single TSWV isolate that was inefficiently transmitted by thrips. Three of the 30 SLIs were transmitted by thrips, and 27 were not. Sequence analysis of the M RNA, thrips transmissibility assays, GC protein analysis, and transmission electron microscopic studies revealed that a specific nonsynonymous mutation (C1375A) in the GN͞GC ORF of the M RNA resulted in the loss of thrips transmissibility without inhibition of virion assembly. This was in contrast to other nontransmissible SLIs, which had frameshift and͞or nonsense mutations in the GN͞GC ORF but were defective in virion assembly. The G C glycoprotein was detectable in the C1375A mutants but not in the frameshift or nonsense mutants. We report a specific viral determinant associated with virus transmission by thrips. In addition, the loss of transmissibility was associated with the accumulation of defective haplotypes in the population, which are not transmissible by thrips, rather than with the presence of a dominant haplotype that is inefficiently transmitted by thrips. These results also indicate that the glycoproteins may not be required for TSWV infection of plant hosts but are required for transmissibility by thrips.

show abstract

Role of La Crosse virus glycoproteins in attachment of virus to host cells

Cited by 77 publications

References 19 publications

Cellular Localization and Antigenic Characterization of Crimean-Congo Hemorrhagic Fever Virus Glycoproteins

Cellular Localization and Antigenic Characterization of Crimean-Congo Hemorrhagic Fever Virus Glycoproteins

The Plant Virus Tomato Spotted Wilt Tospovirus Activates the Immune System of Its Main Insect Vector, Frankliniella occidentalis

Viral genetic determinants for thrips transmission of Tomato spotted wilt virus

Contact Info

Product

Resources

About