Role of key genetic mutations on increasing migration of brain cancer cells through confinement

Bui, Loan; Bhuiyan, Sayem H; Hendrick, Alissa; Chuong, C. J.; Kim, Young Tae

doi:10.1007/s10544-017-0197-9

Cited by 6 publications

(7 citation statements)

References 41 publications

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“…Vimentin provides resistance against stress such as mechanical stress, hypoxia, etc [22][23][24]; and β3-Tubulin is greatly involved in cellular division and intracellular transportation. We also knew that cells inside a microchannel had to encounter hydrodynamic resistances in order to continue their migration journey [13]. Here, the migrating cells enduring confinement were found to reduce β3-Tubulin compared to 2D cells.…”

Section: Discussionmentioning

confidence: 90%

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Microchannel device for proteomic analysis of migrating cancer cells

Bui

Shen

Hill

et al. 2018

Biomed. Phys. Eng. Express

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Tissue invasion and metastasis are leading causes of death among cancer patients due to cells escaping from the primary tumor and invading distant sites. To better understand these phenomena and develop efficient therapeutic regimens against different types of malignancies, there is a need for exclusive cellular and molecular examination of migrating cells. In this study, aggressive brain cancer cells, G55, migrating through confined microchannels were directly extracted and used for subsequent proteomic analysis via Western Blot and/or immunostaining quantification. The method was based on an engineered Polydimethylsiloxane microchannel platform that facilitated the exclusive extraction of migrating cells and their contents while preventing non-migrating (or proliferatingdenoted as 2D) cell contamination. The migrating cells in physical confinement of the microchannels were exclusively examined for their protein expression. They were found with increased expression of Vimentin, approximately 2.5-fold higher than 2D cells. On the other hand, the migrating cells showed significantly decreased β3-Tubulin and Met signal compared to 2D cells. The differences in biomarker expression between migrating cells and non-migrating cells revealed by this study provided an insight into key features of cancer invasion and metastasis. The successful outcome of this research suggests improved targets for ceasing different types of malignancies. REVISED

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Section: Discussionmentioning

confidence: 90%

“…Fabrication of the microchannel devices was conducted based on standard photolithography and soft lithography which had been described in our previous works [4,13,14]. Briefly, we photo-patterned the microchannel feature on a silicon wafer coated with SU-8 photoresist (Microchem Corp, Newton, MA).…”

Section: Fabrication Of Microchannel Devicesmentioning

confidence: 99%

Microchannel device for proteomic analysis of migrating cancer cells

Bui

Shen

Hill

et al. 2018

Biomed. Phys. Eng. Express

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“…The design was sent for transparent mask fabrication (Fine Line Imaging, 50,000 dpi). By conducting standard photolithography technology ( Bui et al, 2017 ; Bui et al, 2018 ; Hobbs et al, 2021 ), we generated a silicon mold (University Wafer, 3,774) with SU8-2025 negative photoresist features (MicroChem). The photoresist was spin-coated on a silicon wafer at 1,800 rpm for 30 s, then soft-baked at 65°C for 3 min and 95°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Development of a tomato xylem-mimicking microfluidic system to study Ralstonia pseudosolanacearum biofilm formation

Chu,

Laxman,

Abdelhamed

et al. 2024

Front. Bioeng. Biotechnol.

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The bacterial wilt pathogen Ralstonia pseudosolanacearum (Rps) colonizes plant xylem vessels and blocks the flow of xylem sap by its biofilm (comprising of bacterial cells and extracellular material), resulting in devastating wilt disease across many economically important host plants including tomatoes. The technical challenges of imaging the xylem environment, along with the use of artificial cell culture plates and media in existing in vitro systems, limit the understanding of Rps biofilm formation and its infection dynamics. In this study, we designed and built a microfluidic system that mimicked the physical and chemical conditions of the tomato xylem vessels, and allowed us to dissect Rps responses to different xylem-like conditions. The system, incorporating functional surface coatings of carboxymethyl cellulose-dopamine, provided a bioactive environment that significantly enhanced Rps attachment and biofilm formation in the presence of tomato xylem sap. Using computational approaches, we confirmed that Rps experienced linear increasing drag forces in xylem-mimicking channels at higher flow rates. Consistently, attachment and biofilm assays conducted in our microfluidic system revealed that both seeding time and flow rates were critical for bacterial adhesion to surface and biofilm formation inside the channels. These findings provided insights into the Rps attachment and biofilm formation processes, contributing to a better understanding of plant-pathogen interactions during wilt disease development.

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“…Using fabricated microfluidic devices with narrow channels as the model platform, we studied GBMs migration in confinement. PDMS (Dow Corning, Sylgard 184) devices with microchannel of 5 × 5 µm cross section and a length of 530 µm connecting two reservoirs each at 100 µm height were fabricated using photo and soft lithography technique (Bui et al 2017 ). A silicone elastomer base was mixed with a curing agent at a mixing ratio of 10:1.…”

Section: Methodsmentioning

confidence: 99%

Differences in creep response of GBM cells migrating in confinement

Khan

Bui

Bachoo

et al. 2020

International Biomechanics

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Using a microfluidic platform to apply negative aspiration pressure (–20, –25, –30, –35 and –40 cm H 2 O), we compared the differences in creep responses of Glioblastoma Multiforme (GBM) cells while migrating in confinement and at a stationary state on a 2D substrate. Cells were either migrating in a channel of 5 x 5 μm cross-section or stationary at the entrance to the channel. In response to aspiration pressure, we found actively migrating GBM cells exhibited a higher stiffness than stationary cells. Additionally, migrating cells absorbed more energy elastically with a relatively small dissipative energy loss. At elevated negative pressure loads up to – 30 cm H 2 O, we observed a linear increase in elastic deformation and a higher distribution in elastic storage than energy loss, and the response plateaued at further increasing negative pressure loads. To explore the underlying cause, we carried out immuno-cytochemical studies of these cells and found a polarized actin and myosin distribution at the front and posterior ends of the migrating cells, whereas the distribution of the stationary group demonstrated no specific regional differences. These differences in creep response and cytoskeletal protein distribution demonstrate the importance of a migrating cell’s kinematic state to the mechanism of cell migration.

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Role of key genetic mutations on increasing migration of brain cancer cells through confinement

Cited by 6 publications

References 41 publications

Microchannel device for proteomic analysis of migrating cancer cells

Microchannel device for proteomic analysis of migrating cancer cells

Development of a tomato xylem-mimicking microfluidic system to study Ralstonia pseudosolanacearum biofilm formation

Differences in creep response of GBM cells migrating in confinement

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