Role of intracellular calcium stores in contrast medium—induced renal vasoconstriction

Drescher, Peter; Rauch, Dieter; Madsen, Paul O.

doi:10.1016/s1076-6332(96)80298-4

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…[8] It has been showed that LPS and GE injected simultaneously in rats induced more toxicity than each substance alone, [1] resulting in decreased glomerular filtration rate and a vasoconstrictor response, which was mediated by extracellular calcium. [7,3] Experimental model of septicemia in rats using LPS showed that it potentiated the toxic effects of GE. [1] [Ca 2þ ] i can be involved in these mechanisms since the acute dose GE increased [Ca 2þ ] i in both alone or LPS or HE treated cells.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Gentamicin, Lipopolysaccharide, and Contrast Media on Immortalized Proximal Tubular Cells

Cunha

Schor

2002

Renal Failure

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Aminoglycosides are widely used in the treatment of gram-negative bacterial infections. Gentamicin (GE) acts mainly in proximal tubular cells, where it is uptake via organic anion transport system and it induces a high incidence of nephrotoxicity, which is characterized by tubular necrosis [5] leading to acute renal failure in 10 to 50% of patients. Gram-negative bacteria has lipopolysaccharide (LPS) which is an endotoxin that cause renal damage. [1] Moreover, many patients are undergone exams using radiologic contrast, which is a risk factor to induce a hemodynamic change in the kidney and to develop acute renal failure. [6] Intracellular calcium [Ca2+]i is involved in renal cellular injury [7,3] and maybe mediate the effects provoked by these drugs. This study was performed to evaluate necrosis, apoptosis, and intracellular calcium levels ([Ca2+]i) in LLC-PK1 (epithelial cell line from pig kidney) induced by GE associated with LPS and a low-osmolality media, Hexabrix (HE).

show abstract

Section: Discussionmentioning

confidence: 99%

“…[1] Moreover, many patients are undergone exams using radiologic contrast, which is a risk factor to induce a hemodynamic change in the kidney and to develop acute renal failure. [6] Intracellular calcium [Ca 2þ ] i is involved in renal cellular injury [7,3] and maybe mediate the effects provoked by these drugs.…”

Section: Introductionmentioning

confidence: 99%

Effects of Gentamicin, Lipopolysaccharide, and Contrast Media on Immortalized Proximal Tubular Cells

Cunha

Schor

2002

Renal Failure

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show abstract

“…The mechanism for the EDTA‐effect in Ca 2+ ‐free medium is less obvious. Presumably, as observed for smooth muscle cells [24], NP may induce intracellular release of Ca 2+ in MNC. This release should not be prevented by EDTA, which is considered to remain extracellular.…”

Section: Resultsmentioning

confidence: 86%

Separation of Human Lymphocytes from Citrated Blood by Density Gradient (NycoPrep) Centrifugation: Monocyte Depletion Depending upon Activation of Membrane Potassium Channels

Bøyum¹,

Fjerdingstad²,

Martinsen³

et al. 2002

Scandinavian Journal of Immunology

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Routine one-step centrifugation procedures (Lymphoprep LP, Percoll) commonly used for separation of blood cells split the cells into two major fractions. After centrifugation the mononuclear cells (MNC monocytes and lymphocytes) are located on the top of the separation fluid, whereas erythrocytes and granulocytes have sedimented to the bottom. We now show that a relatively pure lymphocyte suspension can be obtained by one-step centrifugation of citrated blood by using NycoPrep (NP iohexol), a nonionic X-ray contrast agent. With this gradient medium also the monocytes pass to the bottom, leaving lymphocytes on the top. In parallel separations with LP, which contains Ficoll and a fully dissociated sodium salt of a contrast medium, the results were as usual, i.e. $70±85% lymphocytes and 30±15% monocytes in the top fraction. The monocyte depletion with NP depended upon the use of citrated (ACD) blood and a proper balance of density and osmolality of the gradient medium, and was enhanced by 20 min preincubation with CaCl 2 at room temperature. Monocyte depletion could not be obtained with LP. Under optimal conditions (density 1.075 g/ml, osmolality 280±300 mOsm/kg), the monocyte admixture amounted to $1 (0±2)%, in separations with buffy coat samples. For freshly drawn blood, it was necessary to slightly modify the NP solution. The monocyte depletion was counteracted by blockers of K channels or by KCl in the cell suspension. Following incubation in NP of Percoll-separated cells, an enhanced release of K was observed. The results are interpreted as follows: NP mediates the opening of K channels of MNC, which leads to efflux of K , accompanied with associated anions (Cl ± ). This reduces the osmolality inside the cells which therefore expel water to maintain osmotic equilibrium. In this regard it appears that monocytes are more sensitive than lymphocytes, their density therefore increasing more, so that they are able to pass the density barrier otherwise exerted by the gradient medium.

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“…[8] It has been shown that LPS and GE injected simultaneously in rats induced more toxicity than each substance alone, [1] resulting in decreased glomerular filtration rate and a vasoconstrictor response, which was mediated by extracellular calcium. [3,7] Experimental model of septicemia in rats using LPS showed that it potentiated the toxic effects of GE. [1] [Ca 2þ ] i can be involved in these mechanisms since the acute dose GE increased [Ca 2þ ] i in both alone or LPS or HE treated cells.…”

Section: Discussionmentioning

confidence: 99%

“…[1] Moreover, many patients are undergone exams using radiologic contrast, which is a risk factor to induce a hemodynamic change in the kidney and to develop acute renal failure. [6] Intracellular calcium [Ca 2þ ] i is involved in renal cellular injury [3,7] and maybe mediate the effects provoked by these drugs.…”

Section: Introductionmentioning

confidence: 99%

Effects of Gentamicin, Lipopolysaccharide, and Contrast Media on Immortalized Proximal Tubular Cells

Cunha

Schor

2002

Renal Failure

View full text Add to dashboard Cite

Aminoglycosides are widely used in the treatment of gram-negative bacterial infections. Gentamicin (GE) acts mainly in proximal tubular cells, where it is uptake via organic anion transport system and it induces a high incidence of nephrotoxicity, which is characterized by tubular necrosis leading to acute renal failure in 10 to 50% of patients. Gram-negative bacteria have lipopolysaccharide (LPS) which is an endotoxin that causes renal damage. Moreover, many patients are undergone exams using radiologic contrast, which is a risk factor to induce a hemodynamic change in the kidney and to develop acute renal failure. Intracellular calcium [Ca2+]i is involved in renal cellular injury and maybe mediate the effects provoked by these drugs. This study was performed to evaluate necrosis, apoptosis and intracellular calcium levels ([Ca2+]i) in LLC-PK1 (epithelial cell line from pig kidney) induced by GE associated with LPS and a low-osmolality media, Hexabrix (HE).

show abstract

Role of intracellular calcium stores in contrast medium—induced renal vasoconstriction

Cited by 6 publications

References 34 publications

Effects of Gentamicin, Lipopolysaccharide, and Contrast Media on Immortalized Proximal Tubular Cells

Effects of Gentamicin, Lipopolysaccharide, and Contrast Media on Immortalized Proximal Tubular Cells

Separation of Human Lymphocytes from Citrated Blood by Density Gradient (NycoPrep) Centrifugation: Monocyte Depletion Depending upon Activation of Membrane Potassium Channels

Effects of Gentamicin, Lipopolysaccharide, and Contrast Media on Immortalized Proximal Tubular Cells

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