Role of intestinal brush border peptidases in the simulated digestion of milk proteins

Picariello, Gianluca; Miralles, Beatriz; Mamone, Gianfranco; Sánchez-Rivera, Laura; Recio, Isidra; Addeo, Francesco; Ferranti, Pasquale

doi:10.1002/mnfr.201400856

Cited by 83 publications

(61 citation statements)

References 29 publications

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“…The DH value measured after gastrointestinal digestion with our model was lower than that determined by Picariello et al (2015) which found a degree of hydrolysis for skimmed milk between 34.5 and 58 depending on the substrate to digestive enzyme ratio. Simulated digestion of isolated κ-and β-casein resulted in a hydrolysis degree 3.2 ABTS + radical scavenging activity of digested whole, semi-skimmed and skimmed milk All three types of milk showed ABTS + radical scavenging activity before the digestion (Table 2), but with some differences.…”

Section: Resultsmentioning

confidence: 48%

Bovine milk antioxidant properties: effect of in vitro digestion and identification of antioxidant compounds

Tagliazucchi

Helal

Verzelloni

et al. 2016

Dairy Sci. & Technol.

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Milk proteins contained encrypted in their sequence biologically active components that can be released by enzymatic hydrolysis. Among the biological activities recognized in milk components, the antioxidant activity is of great interest.\ud The objective of the present study was to analyse the antioxidant properties of whole, semi-skimmed and skimmed milk during simulated gastrointestinal digestion and to identify the compounds responsible for the antioxidant activity. Simulated digestion\ud increased the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS)+ radical scavenging activity of milk. In digested whole milk, the main contribution to ABTS+ radical scavenging activity was due to high molecular weight fraction (>3 kg.mol−1).\ud For semi-skimmed and skimmed milk, the main contribution was due to low molecular weight fraction (<3 kg.mol−1). Twelve major peaks were collected from low molecular weight fraction of digested skimmed milk by reversed-phase high-performance liquid chromatography and evaluated for their ABTS+ radical scavenging activity. Among the different fractions, three (F2, F3 and F5) showed high ABTS+ and hydroxyl radical scavenging activity and lipid peroxidation inhibitory capacity. The compounds (free\ud amino acids and peptides) present in these fractions were identified with nano-LCQTOF MS/MS analysis. The amino acids tryptophan and tyrosine seemed fundamental in the ABTS+ and hydroxyl radical scavenging capacities whereas the amino acids\ud phenylalanine and histidine played an important role in the lipid peroxidation inhibitory activity of the peptides. The results reported in this study suggested that milk proteins could act as a carrier for the delivery of antioxidant compounds in the gastrointestinal tract possibly protecting the gastrointestinal tract itself from the oxidative damage

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Section: Resultsmentioning

confidence: 48%

Bovine milk antioxidant properties: effect of in vitro digestion and identification of antioxidant compounds

Tagliazucchi

Helal

Verzelloni

et al. 2016

Dairy Sci. & Technol.

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“…The α s1 -casein (f180-197) was found in an independent sample and it probably survived digestion, because it belongs to a relatively proteolysis-resistant region of the protein. 35 No additional casein peptides were detected, most likely due to their prompt and extensive degradation by the gastro-intestinal system of the mother. The MS/MS spectrum of β-Lg (f42-57) is shown in Figure 1 with the assignment of the most intense fragments, also manually validated.…”

Section: Resultsmentioning

confidence: 98%

Antibody-independent identification of bovine milk-derived peptides in breast-milk

et al. 2016

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Exclusively breast-fed infants can exhibit clear signs of IgE or non IgE-mediated cow's milk allergy. However, the definite characterization of dietary cow's milk proteins (CMP) that survive the maternal digestive tract to be absorbed into the bloodstream and secreted into breast milk remains missing. Herein, we aimed at assessing possible CMP-derived peptides in breast milk. Using high performance liquid chromatography (HPLC)-high resolution mass spectrometry (MS), we compared the peptide fraction of breast milk from 12 donors, among which 6 drank a cup of milk daily and 6 were on strict diary-free diet. We identified two bovine β-lactoglobulin (β-Lg, 2 out 6 samples) and one αs1-casein (1 out 6 samples) fragments in breast milk from mothers receiving a cup of bovine milk daily. These CMP-derived fragments, namely β-Lg (f42-54), (f42-57) and αs1-casein (f180-197), were absent in milk from mothers on dairy-free diet. In contrast, neither intact nor hydrolyzed β-Lg was detected by Western blot and competitive ELISA in any breast milk sample. Eight additional bovine milk-derived peptides identified by software-assisted MS were most likely false positive. The results of this study demonstrate that CMP-derived peptides rather than intact CMP may sensitize or elicit allergic responses in the neonate through mother's milk. Immunologically active peptides from the maternal diet could be involved in priming the newborn's immune system, driving tolerogenic response.

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“…Some precursors included in the β-casein 57-68 region were found when cheese or milk were digested with pepsin and pancreatin (Hernandez-Ledesma, Quiros et al 2007; Sanchez-Rivera, Diezhandino et al 2014) or with human gastrointestinal secretions (Qureshi, Vegarud et al 2013). Interestingly, an additional hydrolysis step with brush border peptidases released β-casomorphin-7 (Picariello et al, 2015).…”

Section: Comparison Of Bioactive Sequences Found In Vitro and In Vivomentioning

confidence: 99%

Correlation between in vitro and in vivo data on food digestion. What can we predict with static in vitro digestion models?

Bohn

Carrière

Day

et al. 2017

Critical Reviews in Food Science and Nutrition

272

153

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During the last decade, there has been a growing interest in understanding food's digestive fate in order tostrengthen the possible effects of food on human health. Ideally, food digestion should be studied in vivoon humans but this is not always ethically and financially possible. Therefore, simple in vitro digestionmodels mimicking the gastrointestinal tract have been proposed as alternatives to in vivo experiments.Thus, it is no surprise that these models are increasingly used by the scientific community, although theirvarious limitations to fully mirror the complexity of the digestive tract. Therefore, the objective of thisarticle was to call upon the collective experiences of scientists involved in Infogest (an internationalnetwork on food digestion) to review and reflect on the applications of in vitro digestion models, theparameters assessed in such studies and the physiological relevance of the data generated when comparedto in vivo data. The authors provide a comprehensive review in vitro and in vivo digestion studiesinvestigating the digestion of macronutrients (i.e. proteins, lipids and carbohydrates) as well as studies of the bioaccessibility and bioavailability of micronutrients and phytochemicals. The main conclusion is thatevidences show that despite the simplicity of in vitro models they are often very useful in predictingoutcomes of the digestion in vivo. However, this has relies on the complexity of in vitro models and theirtuning towards answering specific questions related to human digestion physiology, which leaves a vastroom for future studies and improvements

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Role of intestinal brush border peptidases in the simulated digestion of milk proteins

Abstract: Any in vitro digestion model should be supplemented with a jejunal phase to realistically determine the bioaccessibility and bioavailability of dietary peptides.

Cited by 83 publications

References 29 publications

Bovine milk antioxidant properties: effect of in vitro digestion and identification of antioxidant compounds

Bovine milk antioxidant properties: effect of in vitro digestion and identification of antioxidant compounds

Antibody-independent identification of bovine milk-derived peptides in breast-milk

Correlation between in vitro and in vivo data on food digestion. What can we predict with static in vitro digestion models?

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