Role of interleukin-6 in T-cell activation during primary and secondary infection with Listeria monocytogenes

Liu, Zhanqi; Simpson, R. J.; Cheers, Christina

doi:10.1128/iai.63.7.2790-2792.1995

Cited by 11 publications

(10 citation statements)

References 9 publications

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“…It has been previously shown that IL-6 is needed for the development of protective T cells against intracellular parasite infections such as M. avium [46], L. monocytogenes [47] and M. tuberculosis [48]. In some of these works, IL-6 was shown to be necessary for the development of a Th1 response as assessed by the ability of the antigenspecific T cells to secrete IFN-γ [49,50]. In this context, we suggest that Nattectin antigen initiates a pro-inflammatory response and is able to induce the recruitment of macrophages, which depends on a microenvironment containing IL-4 and IFN-γ.…”

Section: Discussionmentioning

confidence: 99%

Role of interplay between IL-4 and IFN-γ in the in regulating M1 macrophage polarization induced by Nattectin

Ishizuka

Ferreira

Grund

et al. 2012

International Immunopharmacology

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Recently our group described that Nattectin, a C-type lectin of the venom of Thalassophryne nattereri shows a potent pro-inflammatory capacity. Here, we demonstrated that Nattectin is able to induce M1 macrophage marker iNOS, and up-regulate the expression of MHC class II, CD80, CD86 and CD40 molecules. The increase in MHC class II and CD49a integrin expression with MMP-9 production and endocytic capacity depend on lectin function of Nattectin. Moreover, the polarization of peritoneal and bone marrow-derived macrophages induced by Nattectin to M1 profile is dependent on Th1 cytokines (IL-12 and IFN-γ), and negatively regulated by Th2 cytokines (IL-4, IL-10 and IL-13). Also we reveal that IL-4 play a dual role in this polarization: a regular action of IL-4 was seen in the negative regulation of the CD40 expression, but an unexpected positive regulation was seen in the expression of CCR7 and MHC class II. Finally, our in vivo studies showed that the influx of neutrophils and small peritoneal macrophage--F4/80(low)MHCII(hi) induced by Nattectin is totally dependent on IL-4 and IFN-γ cytokines. Furthermore, the induction of IL-6 release is negatively regulated by IL-4 and positively regulated by IL-12 and IFN-γ. Together, the results allowed us to expand the knowledge about the regulation of macrophage activation, as well as confirmed the ability of Nattectin, a fish C-type lectin, as an important immunomodulatory agent.

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Section: Discussionmentioning

confidence: 99%

Role of interplay between IL-4 and IFN-γ in the in regulating M1 macrophage polarization induced by Nattectin

Ishizuka

Ferreira

Grund

et al. 2012

International Immunopharmacology

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“…The precise role of IL-6 in T-cell differentiation is far from clarified. Deficient priming of IFN-␥ responses in the absence of IL-6 was reported during infections by L. monocytogenes or M. tuberculosis (23,(25)(26)(27). However, others have shown that IL-6 can promote Th2 responses through the induction of IL-4 (37).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, IL-4 has the opposite effect by decreasing the expression of the beta 2 chain of the IL-12 receptor, thereby preventing the action of IL-12 on the T-helper-cell precursors (40,47). The role of IL-6 is unclear since it has been shown that this cytokine is required for the induction of protective Th1 cells during experimental infections by Mycobacterium avium (5), M. tuberculosis (23), and Listeria monocytogenes (25)(26)(27), whereas others have shown that IL-6 is involved in the generation of Th2 responses (37). Additionally, it has been shown that IL-6 can act on the infected macrophages harboring mycobacteria and promote mycobacterial growth (12,44) or antagonize the effects of bacteriostasis-inducing cytokines such as tumor necrosis factor alpha (7).…”

mentioning

confidence: 99%

Interleukin-6 and Interleukin-12 Participate in Induction of a Type 1 Protective T-Cell Response during Vaccination with a Tuberculosis Subunit Vaccine

et al. 1999

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We examined the role of cytokines in the development of gamma interferon (IFN-γ)-secreting protective T cells following immunization with a culture filtrate subunit vaccine againstMycobacterium tuberculosis containing the adjuvant dimethyldioctadecylammonium bromide (DDA). Depletion of either interleukin-6 (IL-6) or IL-12 with specific neutralizing antibodies during vaccination reduced the priming of T cells for antigen-specific proliferation and IFN-γ secretion. Such reduction was also observed in IL-6 gene-disrupted mice as compared to wild-type animals. IL-6 was found to play a role in the initial differentiation of Th1 cells but not in their expansion. The defect found after IL-6 depletion or in IL-6-knockout mice was compensated by the inclusion of recombinant mouse IL-12 in the vaccine. The induction of protective immunity against an intravenous or an aerosol challenge with live, virulentM. tuberculosis was markedly reduced by neutralizing either IL-6 or IL-12 during immunization with the vaccine. Likewise, the effects of IL-6 neutralization were partially reversed by including IL-12 in the vaccine. Our data point to an important role of IL-6 and IL-12 in the generation of cell-mediated immunity to tuberculosis.

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“…10 In addition, it has been shown that despite being necessary for the development of such cells, once the cells are differentiated the cytokine is no longer needed. 9,11,14 In some of these works, IL-6 was shown to be necessary for the development of a Th1 response as assessed by the ability of the antigen-speci®c T cells to secrete IFN-c. 11,14 We have previously demonstrated that the presence of IL-6 was necessary for the development of a T-cell response to a subunit vaccine able to protect against tuberculosis. 7 Here we show that the emergence of IFN-c-secreting CD4 + T cells during the ®rst days that followed a primary immunization was severely hampered in the absence of IL-6.…”

Section: Discussionmentioning

confidence: 99%

Interleukin‐6 regulates the phenotype of the immune response to a tuberculosis subunit vaccine

et al. 2001

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Summary We investigated the role of interleukin‐6 (IL‐6) in the development of the immune response to a subunit vaccine against tuberculosis consisting of the culture filtrate proteins of Mycobacterium tuberculosis emulsified in the adjuvant dimethyldioctadecylammonium bromide (DDA). C57Bl/6 mice immunized with this vaccine developed a strong T helper 1 (Th1) response characterized by an increased production of interferon‐γ (IFN‐γ) secreted by CD4+ T cells. Neutralization of IL‐6 during in vivo priming resulted in marked reduction in the ability of T cells to secrete IFN‐γ and IL‐2 and to proliferate. IL‐6 gene‐disrupted mice primed with the vaccine showed a decrease in the number of IFN‐γ‐producing cells and an increase in IL‐4‐secreting cells as compared to control mice. In contrast, neutralization of IL‐6 during a boost of the vaccine in previously primed mice did not affect the development of IFN‐γ‐producing cells but still increased the number of IL‐4‐producing cells. Our work shows that IL‐6 plays a major role in the priming but not in the later expression of a Th1 response to a tuberculosis vaccine.

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Role of interleukin-6 in T-cell activation during primary and secondary infection with Listeria monocytogenes

Cited by 11 publications

References 9 publications

Role of interplay between IL-4 and IFN-γ in the in regulating M1 macrophage polarization induced by Nattectin

Role of interplay between IL-4 and IFN-γ in the in regulating M1 macrophage polarization induced by Nattectin

Interleukin-6 and Interleukin-12 Participate in Induction of a Type 1 Protective T-Cell Response during Vaccination with a Tuberculosis Subunit Vaccine

Interleukin‐6 regulates the phenotype of the immune response to a tuberculosis subunit vaccine

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