Role of Interleukin-22 in ulcerative colitis

Zhao, Nan; Liu, Chuanguo; Li, Ning; Zhou, Shuang; Guo, Yuting; Shen, Yang; Liu, Huimin

doi:10.1016/j.biopha.2023.114273

Cited by 7 publications

(3 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Berberine, as an important metabolite of BTW, has been extensively studied as an anti-autoimmune agent and has been reported to be a promising candidate for the treatment of UC (Li et al, 2016;Yang et al, 2023b). Anemoside B4 could prevent chronic relapsing colitis in mice by modulating the inflammatory response, the colonic transcriptome, and the gut microbiota (Han et al, 2022;Feng et al, 2023;Zhao et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

“…Both the results of western blot (Figure 4A) and proteomics (Figure 4B) indicated that the relative expression levels of DDC were decreased in the colon of UC mice, but were significantly increased after BTW treatment. AHR activation-induced IL-22 production is important Frontiers in Pharmacology frontiersin.org Frontiers in Pharmacology frontiersin.org for maintaining epithelial cell integrity (Meynier et al, 2022;Zhao et al, 2023). The ELISA results showed that IL-22 was downregulated in colon tissue from the model group, while BTW reversed the DSS-induced reduction in IL-22 (Figure 4C).…”

Section: Btw Activates the Ahr/il-22 Signaling Pathway Through Upregu...mentioning

confidence: 93%

See 1 more Smart Citation

Baitouweng decoction alleviates ulcerative colitis by regulating tryptophan metabolism through DOPA decarboxylase promotion

Zhang,

Lin,

Zhang

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

BackgroundBaitouweng decoction (BTW) is a classic botanical drugs formula that has been widely used clinically for the treatment of gut-related disorders in China. However, its role in ameliorating ulcerative colitis (UC) remains to be explored.PurposeThe study aimed to determine the therapeutic efficacy and potential mechanism of action of BTW on dextran sodium sulfate (DSS)-induced colitis mice.MethodsIn vivo: 3.5% DSS-induced experimental colitis mice were treated with BTW (Pulsatilla chinensis (Bunge) Regel, Phellodendron chinense C. K. Schneid, Coptis chinensis Franch and Fraxinus chinensis Roxb), kynurenine or DOPA decarboxylase (DDC) inhibitor (carbidopa). In vitro: Caco-2 cells were stimulated with TNF-α to activate inflammation and later treated with various concentrations of BTW and carbidopa. Model evaluation included body weight, disease activity index (DAI) score, colon length and histopathology. Cytokine levels were measured by flow cytometry. Protein levels were analyzed by proteomics and functionally annotated. The levels of tryptophan metabolites in mouse serum and colon were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Alcian Blue/Phosphate Acid Schiff (AB/PAS) staining, immunohistochemistry and western blot were used to assess the intestinal barrier function and detect the protein expression levels.ResultsBTW significantly reduced the DAI, ameliorated colonic injury and regulated inflammatory cytokines in DSS-induced colitis mice. The botanical drugs formula also promoted intestinal epithelial barrier repair by enhancing the expression of the tight junction (TJ) proteins. Tryptophan metabolic signaling pathway was significantly enriched in DSS-induced UC mice, and BTW decreased the level of kynurenine, increased indole metabolites. The therapeutic effect of BTW was evidently reduced when kynurenine was given to mice. Also, BTW promoted DDC protein expression and activated the aryl hydrocarbon receptor (AHR)/IL-22 signaling pathway.ConclusionBTW improves ulcerative colitis by promoting DDC expression, regulating the conversion of tryptophan metabolism from the kynurenine pathway to the indole metabolism pathway, thereby modulating tryptophan metabolism to increase indole metabolites, and activating AHR receptors to restore intestinal barrier function.

show abstract