Role of interferon gamma release assay in the diagnosis and management of<i>Mycobacterium tuberculosis</i>-associated uveitis: a review

Rahman, Samra; Irfan, Muhammad; Siddiqui, Rehana

doi:10.1136/bmjophth-2020-000663

Cited by 10 publications

(7 citation statements)

References 61 publications

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“…To our knowledge, several studies have been conducted on the usefulness of monitoring the response to ATT in active or latent TB; however, the exact role of IGRAs in monitoring treatment response in TBU remains undefined. Additionally, no study has reported the accuracy and clinical application of QFT-Plus in TBU [47] . The evidence of using new generations of IGRAs in TBU is still lacking.…”

Section: Role Of Interferon-gamma Release Assays In Tuberculousmentioning

confidence: 99%

Interferon-gamma release assays in tuberculous uveitis: a comprehensive review

Tungsattayathitthan¹,

Tesavibul²,

Dharakul³

et al. 2022

Int J Ophthalmol

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Tuberculous uveitis (TBU) comprises a broad clinical spectrum of ocular manifestations, making its diagnosis challenging. Ophthalmologists usually require evidence from investigations to confirm or support a clinical diagnosis of TBU. Since direct isolation of the causative organism from ocular specimens has limitations owing to the small volume of the ocular specimens, resultant test positivities are low in yield. Immunodiagnostic tests, including the tuberculin skin test and interferon-gamma release assays (IGRAs), can help support a clinical diagnosis of TBU. Unlike the tuberculin skin test, IGRAs are in vitro tests that require a single visit and are not affected by prior Bacillus Calmette-Guerin vaccination. Currently, available IGRAs consist of different techniques and interpretation methods. Moreover, newer generations have been developed to improve the sensitivity and ability to detect active tuberculosis. This narrative review collates salient practice points as a reference for general ophthalmologists, such as evidence for the utilization of IGRAs in patients with suspected TBU, and summarizes basic knowledge and details of clinical applications of these tests in a clinical setting.

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Section: Role Of Interferon-gamma Release Assays In Tuberculousmentioning

confidence: 99%

Interferon-gamma release assays in tuberculous uveitis: a comprehensive review

Tungsattayathitthan¹,

Tesavibul²,

Dharakul³

et al. 2022

Int J Ophthalmol

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“… 7 The IGRA test was introduced in 2001 for the assessment of the IFN‐γ released by T‐cells following stimulation by antigen‐specific to Mycobacterium tuberculosis . 8 Following the COVID‐19 pandemic, the IGRA assays for SARS‐CoV‐2 were also developed. Currently, two types of IGRA tests are available for evaluation of the IFN‐γ released by T‐cells following the COVID‐19 vaccination or SARS‐CoV‐2 infection: Quanti‐FERON (QFN) SARS‐CoV‐2 (QIAGEN) and T SPOT Discovery SARS‐CoV‐2 kit (Oxford Immunotec).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis between STANDARD‐E Covi‐FERON ELISA with pre‐existing IFN‐γ release assays and determination of the optimum cutoff value for assessment of T‐Cell response to SARS‐CoV‐2

Jang

Widyasari

Kim

2023

Clinical Laboratory Analysis

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Background Interferon‐gamma (IFN‐γ) release assays (IGRAs) are useful for the assessment of the T‐cell response to severe acute respiratory syndrome‐coronavirus‐2 (SARS‐CoV‐2). We aimed to assess the performance of the newly developed IGRA ELISA test compared to the pre‐existing assays and to validate the cutoff value in real‐world conditions. Methods We enrolled 219 participants and assessed agreement between STANDARD‐E Covi‐FERON ELISA with Quanti‐FERON SARS‐CoV‐2 (QFN SARS‐CoV‐2), as well as with T SPOT Discovery SARS‐CoV‐2 based on Cohen's kappa‐index. We further determined the optimal cutoff value for the Covi‐FERON ELISA according to the immune response to vaccinations or infections. Results We found a moderate agreement between Covi‐FERON ELISA and QFN SARS‐CoV‐2 before vaccination (kappa‐index = 0.71), whereas a weak agreement after the first (kappa‐index = 0.40) and second vaccinations (kappa‐index = 0.46). However, the analysis between Covi‐FERON ELISA and T SPOT assay demonstrated a strong agreement (kappa‐index >0.7). The cut‐off value of the OS (original spike) marker was 0.759 IU/mL with a sensitivity of 96.3% and specificity of 78.7%, and that of the variant spike (VS) marker was 0.663 IU/mL with a sensitivity and specificity of 77.8% and 80.6%, respectively. Conclusion The newly determined cut‐off value may provide an optimum value to minimize and prevent the occurrence of false‐negative or false‐positive during the assessment of T‐cell immune response using Covi‐FERON ELISA under real‐world conditions.

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“…A wide range of hosts can be infected by mycobacteria, complicating efforts at disease control [ 2 ]. TB, which is still considered a major health threat of public health concern, causes an estimated 8.6 million new cases and 1.3 million deaths annually [ 3 ]. Hence, the rapid and accurate diagnosis of TB is critical.…”

Section: Introductionmentioning

confidence: 99%

Comparative diagnosis of bovine tuberculosis using single intradermal cervical tuberculin technique, conventional methods, enzyme-linked immunosorbent assay, and the gamma-interferon assay

Hekal

Dapgh²,

Abd-Elhafeez

et al. 2022

Vet World

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Background and Aim: Bovine tuberculosis (TB) is a zoonotic disease that causes huge economic losses. This study aimed to compare the result obtained from the single intradermal test, conventional methods (culture and microscopy), gamma-interferon (IFN-γ) assay, and indirect enzyme-linked immunosorbent assay (ELISA) to diagnose bovine TB. Materials and Methods: This study evaluated 2913 animals from milk farms in Cairo, El-Sharkia, and El-Qalyubia Governorates by single intradermal cervical tuberculin technique (SICTT), ELISA, and IFN-γ assay. Results: Of the 2913 dairy cows surveyed, 3.7% yielded positive results. Culture prepared samples on Lowenstein-Jensen and Middlebrook 7H10 agar media yielded 52 (1.85%) isolates of Mycobacterium spp. from 2805 milk samples that yielded negative tuberculin reactions and 56 (51.85%) isolates of Mycobacterium spp. were recovered from 108 lymph node samples from positive cases. ELISA analysis of the sera of 108 positive SICTT reactors revealed that 94 (87.03%) and 97 (89.81%) animals were positive for bovine purified protein derivative (PPD-B) antigen and commercial polypeptide antigen, respectively. IFN-γ assays were performed on whole blood samples collected from positive SICTT reactors and showed that 103 (95.37%) animals were positive. Conclusion: M. tuberculosis complex may be isolated from raw milk and not all infected animals shed mycobacterial bacilli in their milk. The use of polypeptide antigen in ELISA provides better diagnostic efficacy than PPD-B antigen. The IFN-γ assay is more sensitive than both SICTT and ELISA. It should be used in parallel with SICTT to allow the detection of more positive animals before they become a source of infection to other animals and humans.

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Role of interferon gamma release assay in the diagnosis and management ofMycobacterium tuberculosis-associated uveitis: a review

Cited by 10 publications

References 61 publications

Interferon-gamma release assays in tuberculous uveitis: a comprehensive review

Interferon-gamma release assays in tuberculous uveitis: a comprehensive review

Comparative analysis between STANDARD‐E Covi‐FERON ELISA with pre‐existing IFN‐γ release assays and determination of the optimum cutoff value for assessment of T‐Cell response to SARS‐CoV‐2

Comparative diagnosis of bovine tuberculosis using single intradermal cervical tuberculin technique, conventional methods, enzyme-linked immunosorbent assay, and the gamma-interferon assay

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