Role of Integrin-Linked Kinase in Multi-drug Resistance of Human Gastric Carcinoma SGC7901/DDP Cells

Abstract

Gastric carcinoma is a leading cause of cancer death in the world and multi-drug resistance (MDR) is an essential aspect of gastric carcinoma chemotherapy failure. Recent studies have shown that integrin-linked kinase (ILK) is involved in metastasis of human tumors, expression silencing of ILK inhibiting the metastasis of several types of cultured human cancer cells. However, the role and potential mechanism of ILK to reverse the multidrug resistance in human gastric carcinoma is not fully clear. In this report, we focused on roles of expression silencing of ILK in multi-drug resistance reversal of human gastric carcinoma SGC7901/DDP cells, including increased drug sensitivity to cisplatin, cell apoptosis rates, and intracellular accumulation of Rhodamine-123, and decreased mRNA and protein expression of multi-drug resistance gene (MDR1), multi-drug resistanceassociated protein (MRP1), excision repair cross-complementing gene 1 (ERCC1), glutathione S-transferase -π (GST-π) and RhoE, and transcriptional activation of AP-1 and NF-κB in ILK silenced SGC7901/DDP cells. We also found that there was a decreased level of p-Akt and p-ERK. The results indicated that ILK might be used as a potential therapeutic strategy to combat multi-drug resistance through blocking PI3K-Akt and MAPK-ERK pathways in human gastric carcinoma.

Reversal of Resistance towards Cisplatin by Curcumin in Cervical Cancer Cells

“…Despite considerable advances in anticancer drug development, major fundamental obstacle associated with cancer chemotherapy is development of chemo-resistance (Song et al, 2012). Among several intrinsic cellular mechanism associated with the development of multirug resistance (MDR), overexpression of plasma membrane glycoprotein 1 or Pgp1 is considered as one of the most important known mediator of MDR (Szakacs et al, 2006).…”

Knockdown of MDR1 Increases the Sensitivity to Adriamycin in Drug Resistant Gastric Cancer Cells

“…Many drug resistance cell lines including SGC7901/ VCR (Zhang et al, 2012), SGC7901/DDP (Song et al, 2012), SGC7901/ADM have been established to investigate the mechanisms of MDR and to develop the strategies to overcome the MDR in gastric cancer. However, these cell lines were resistant to certain drugs and always lose the property of drug resistance after a few passages.…”

Knockdown of Med19 Suppresses Proliferation and Enhances Chemo-sensitivity to Cisplatin in Non-small Cell Lung Cancer Cells

“…However, inherent and acquired drug resistance limited therapeutic efficacy. It has been shown that cisplatin resistance is associated with multifactorial mechanisms, such as inactivation by glutathione, metallothionein, or other sulphur-containing molecules, increased repair of DNA damage and Autophagy, reduced apoptosis and intracellular drug accumulation by changing the profile of drug-influx or -efflux molecules (Song et al, 2012;Galluzzi et al, 2012;Zhang et al, 2013). Many studies, concerned with the specific gene manipulation, have provided a way to make cancer cells specifically more sensitive to cisplatin (Hu et al, 2012;Li et al, 2012;Yu et al, 2013).…”