Role of insect sex pheromones in mating behavior. V. Profile of process on male flying approach toward sex pheromone source

Hirooka, Yoshitoshi

doi:10.1007/bf02348250

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…molesta vary up to 30% among individuals reared under different conditions; therefore, the different number of sensilla between studies could be related to this factor (Nagy & George 1981). The insignificant difference in zigzag flight may be related to the ratio of E 8–12:Ac to Z 8–12:Ac (Hirooka 1986; Kennedy 1983; Willis & Baker 1988, 1994; Yamanaka et al . 2003).…”

Section: Discussionmentioning

confidence: 99%

Male Grapholita molesta (Busck) adults responding to various component combinations of synthetic female sex pheromone

Guo

Wang

Chai

et al. 2022

Entomological Research

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Grapholita molesta (Busck) is a cosmopolitan fruit pest. The sex pheromones released by females have four components, namely (Z)-8-dodecenyl acetate (Z8-12:Ac), E8-12:Ac, (Z)-8-dodecen-1-ol (Z8-12:OH), and dodecanol (12:OH). This multichemical attractive system has been reported to induce various male behavioral responses, from arousal to directional flight, and enhance trapping. However, the specific male behaviors induced by individual component or component blend is unclear. This study investigated the effects of different combinations of these four components using electroantennogram, wind tunnel assays, and field tests on aspects of male behavioral performance. Our results showed that the (Z8-12:Ac + E8-12:Ac)containing pheromones were mainly responsible for long-distance detection and close-distance location in males, including antennal detection of the stimulus, flight initiation, directional flight, and trapping. The addition of E8-12:Ac to Z8-12:Ac plays an important role in pheromone searching behavior of males in close range. The ternary component, Z8-12:Ac + E8-12: Ac + Z8-12:OH was crucial for capturing male moths, and 12:OH significantly increased the male catch. The addition of Z8-12:OH and 12:OH to Z8-12: Ac + E8-12:Ac plays an important role in completing the whole response sequence in field attraction. This study revealed that Z8-12:Ac + E8-12:Ac acts as a attractant to males, whereas Z8-12:OH, and 12:OH are the synergists that enhance male attraction in the sex pheromone blend.

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Section: Discussionmentioning

confidence: 99%

Male Grapholita molesta (Busck) adults responding to various component combinations of synthetic female sex pheromone

Guo

Wang

Chai

et al. 2022

Entomological Research

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Functional characterization of PBP1 gene in Helicoverpa armigera (Lepidoptera: Noctuidae) by using the CRISPR/Cas9 system

Liu

Han

et al. 2017

Sci Rep

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Pheromone binding proteins (PBPs) are thought to play crucial roles in perception of the sex pheromones particularly in noctuid moths, but this is rarely in vivo evidenced due to lacking an effective technique. Here, we reported an in vivo functional study of PBP1 in the important lepidopteran pest Helicoverpa armigera (HarmPBP1), by using the CRISPR/Cas9 system. Efficient and heritable mutagenesis was achieved by egg injection of mixture of Cas9-mRNA and HarmPBP1-sgRNA. The TA cloning and sequencing revealed various insertion and/or deletion (indel) mutations at the target site. Among those, one mutation resulted in a premature stop codon at the target site, which led to a highly truncated protein with only 10 amino acids. The HarmPBP1 with this mutation would completely loss its function, and thus was used to select the homozygous mutant insects for functional analysis. The electroantennogram recording showed that the mutant male adults displayed severely impaired responses to all three sex pheromone components (Z11-16:Ald, Z9-16:Ald and Z9-14:Ald). Our study provides the first in vivo evidence that HarmPBP1 plays important role in perception of female sex pheromones, and also an effective methodology for using CRISPR/Cas9 system in functional genetic study in H. armigera as well as other insects.

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Role of insect sex pheromones in mating behavior. V. Profile of process on male flying approach toward sex pheromone source

Cited by 2 publications

References 31 publications

Male Grapholita molesta (Busck) adults responding to various component combinations of synthetic female sex pheromone

Male Grapholita molesta (Busck) adults responding to various component combinations of synthetic female sex pheromone

Functional characterization of PBP1 gene in Helicoverpa armigera (Lepidoptera: Noctuidae) by using the CRISPR/Cas9 system

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