Role of Immunomarkers in the Clinicopathological Analysis of Unicystic Ameloblastoma

Abstract

Purpose. The clinical behavior of unicystic ameloblastoma varies according to its subtype. The assessment of its proliferative capacity, neovascularization, and invasiveness using relevant immunomarkers may aid in appropriate surgical therapeutic protocol. Methods. 18 cases of clinically and histologically confirmed unicystic ameloblastoma, categorized as luminal, intraluminal, or mural subtypes, were analyzed retrospectively. Immunomarkers such as Ki-67, CD34, MMP-2, and MMP-9 were studied to evaluate their behavior. Results. Labeling index of Ki-67 was 4.25% in the intraluminal subtype, compared with 2.14% in the luminal and 4.04% in the mural variant (P = 0.3). CD34 immunostaining was significantly higher in the mural variant (43 per high power field) than the other two subtypes (P = 0.04). MMP-2 and MMP-9 were strongly expressed in mural, moderately in intraluminal, and weakly to absent in luminal variant. Conclusions. High proliferative index, angiogenesis, and protease activity in the mural ameloblastoma, ascertained by the expression of these markers, confirm its aggressive phenotype. The intraluminal and luminal subtype exhibiting decreased expression are compatible with their indolent clinical behavior.

Immunohistochemical expression of matrix metalloproteinases in ameloblastomas and pericoronal follicles

“…In the present study, all AM samples showed expressions of MMP‐1, MMP‐2 and MMP‐9 in the epithelium and connective tissues, as has been described by other authors . Although MMP expression has been associated with local invasiveness of AM , this study found significantly lower expressions of MMP‐1 and MMP‐2 in the epithelium of SA when compared with UA and PF cases. Furthermore, there was a significantly lower expression of MMP‐9 found in the stroma of SA and UA when compared with PF.…”

“…Ameloblastoma is an odontogenic neoplasm characterized by local invasiveness and recurrence (1). The mechanisms underlying the local invasiveness have been associated with proteolytic enzymes related to the invasive phenotype, such as MMP (3)(4)(5)(6)(7)(8)(9)(17)(18)(19)(20)(21)(22).…”

Calretinin Expression in Unicystic Ameloblastoma and Odontogenic Cysts

“…It arises from epithelial remnants of the dental lamina, enamel organ, reduced enamel epithelium, epithelial cell rests of Malassez, basal layer of the oral mucosa, as well as the epithelial lining of odontogenic cysts like dentigerous cyst, radicular cyst and odontogenic keratocyst (keratocystic odontogenic tumour) [3] [4]. Unicystic ameloblastoma (UA) is a clinico-pathologically distinct type of ameloblastoma comprising 5% to 22% of all reported cases of intraosseous ameloblastoma [5] [6]. It displays clinical, radiographic or gross features of a jaw cyst, but shows an epithelial lining composed focally or entirely of ameloblastomatous epithelium on histopathologic examination [5] [6].…”

“…Unicystic ameloblastoma (UA) is a clinico-pathologically distinct type of ameloblastoma comprising 5% to 22% of all reported cases of intraosseous ameloblastoma [5] [6]. It displays clinical, radiographic or gross features of a jaw cyst, but shows an epithelial lining composed focally or entirely of ameloblastomatous epithelium on histopathologic examination [5] [6].…”

Evaluation of cell proliferation in cystic lesions associated with impacted third molars

“…Therefore, it is widely used as a diagnostic and prognostic tool due to the association between tumor cell proliferation index and lesion behavior. The proliferation index of tumor cells may represent an important indicator of the aggressiveness and potential recurrence of neoplasia (Metgud & Gupta, 2013;Sah, Menon, Kamath, Chandrashekar, & Carnelio, 2013).…”