Role of Immunology in Defining Transmitter‐Specific Neurons

Rl, Kenigsberg; Cuello, A. Claudio

doi:10.1111/j.1600-065x.1987.tb00536.x

Cited by 6 publications

(1 citation statement)

References 106 publications

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“…Preabsorption of this primary antiserum with the synthetic epitope sequence MOR1 387-398 (L EN L EAETAPL P) at 10 Ϫ4 M overnight at 4°C was used as a control. The other primary antisera used were as follows: a monoclonal mouse anti-Met / Leu-enkephalin (Chemicon, Temecula, CA; 1:250 dilution; characterized by Cuello et al, 1984;Kenigsberg and Cuello, 1987), a monoclonal mouse anti-synaptophysin (Sigma; 1:100 dilution; characterized by Devoto and Barnstable, 1987), and a polyclonal goat anti-PHA-L antibody (Vector Laboratories; 1:100 dilution; characterized by Wouterlood et al, 1990).…”

Section: Methodsmentioning

confidence: 99%

Presynaptic Versus Postsynaptic Localization of μ and δ Opioid Receptors in Dorsal and Ventral Striatopallidal Pathways

Olive¹,

Antón²,

Micevych

et al. 1997

J. Neurosci.

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Parallel studies have demonstrated that enkephalin release from nerve terminals in the pallidum (globus pallidus and ventral pallidum) can be modulated by locally applied opioid drugs. To investigate further the mechanisms underlying these opioid effects, the present study examined the presynaptic and postsynaptic localization of ␦ (DOR1) and (MOR1) opioid receptors in the dorsal and ventral striatopallidal enkephalinergic system using fluorescence immunohistochemistry combined with anterograde and retrograde neuronal tracing techniques. DOR1 immunostaining patterns revealed primarily a postsynaptic localization of the receptor in pallidal cell bodies adjacent to enkephalin-or synaptophysin-positive fiber terminals. MOR1 immunostaining in the pallidum revealed both a presynaptic localization, as evidenced by punctate staining that co-localized with enkephalin and synaptophysin, and a postsynaptic localization, as evidenced by cytoplasmic staining of cells that were adjacent to enkephalin and synaptophysin immunoreactivities. Injections of the anterograde tracer Phaseolus vulgaris leucoagglutinin (PHA-L) or the retrograde tracer Texas Red-conjugated dextran amine (TRD) into the dorsal and ventral striatum resulted in labeling of striatopallidal fibers and pallidostriatal cell bodies, respectively. DOR1 immunostaining in the pallidum co-localized only with TRD and not PHA-L, whereas pallidal MOR1 immunostaining co-localized with PHA-L and not TRD. These results suggest that pallidal enkephalin release may be modulated by opioid receptors located presynaptically on striatopallidal enkephalinergic neurons and by ␦ opioid receptors located postsynaptically on pallidostriatal feedback neurons.

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Section: Methodsmentioning

confidence: 99%

Presynaptic Versus Postsynaptic Localization of μ and δ Opioid Receptors in Dorsal and Ventral Striatopallidal Pathways

Olive¹,

Antón²,

Micevych

et al. 1997

J. Neurosci.

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Morphological characterization of substance P‐like immunoreactive glomeruli in the superficial dorsal horn of the rat spinal cord and trigeminal subnucleus caudalis: A quantitative study

Ribeiro‐da‐Silva

Tagari

Cuello

1989

J of Comparative Neurology

132

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The aim of this work was to study the ultrastructural distribution of substance P-like immunoreactivity in laminae I and II of rat spinal cord and trigeminal subnucleus caudalis in relation to synaptic glomeruli. A bispecific monoclonal antibody directed against substance P and horseradish peroxidase was used, combining sensitive immunocytochemistry with preservation of fine ultrastructural detail. Some of the quantitative observations were carried out with an automated image analysis system. The study revealed that in lamina I of the spinal cord, almost all immunoreactive profiles counted were nonglomerular, and a considerable number of them contacted medium-size or large dendrites or were in direct contact with other vesicle-containing profiles. In ventral lamina II, 9.4% of the labeled axonal varicosities were central boutons of type I glomeruli (CI). They could be identified by their scalloped contour, number and types of peripheral profiles, reduced density of mitochondria, and localization in the dorsal horn. However, these immunoreactive glomerular CI boutons (14.1% of the total number of CI) differed statistically from the prevailing population of nonimmunoreactive CI, by being surrounded by less peripheral neuronal profiles, which established fewer synapses. In addition, they contained more than three dense-core vesicles per central profile. In the trigeminal subnucleus caudalis laminae I and II, the substance P fibers and varicosities had a plexiform orientation at the light microscopic level, which contrasted with the mainly rostrocaudal orientation of the spinal cord's lamina II plexus. However, the main ultrastructural findings were similar. These results demonstrate that substance P-like immunoreactivity occurs in a large number of type I synaptic glomeruli with specific morphological features and reinforce the current concept that the substantia gelatinosa of the spinal cord and trigeminal subnucleus caudalis are homologous structures.

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The production of a ?universal developer? for the immunological detection of human IgG and its application in immunodiagnostics

1988

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This study describes the development of a biospecific monoclonal antibody capable of the simultaneous recognition of horseradish peroxidase (HRP) and human IgG. This antibody, coded McC2, has been applied in a novel manner as a universal developing reagent for the detection of human IgG. McC2 cross-reacts with all human IgG subtypes and was found to recognise an epitope on the Fc portion of human IgG. McC2 does not cross-react with human IgM or IgA. This bi-specific antibody belongs to the mouse IgG1 subclass. McC2 was used for the detection of human IgG in a simple one step enzyme-linked immunosorbent assay (ELISA). Use of this bi-specific antibody in this assay resulted in an excellent signal to noise ratio with background in negative control wells virtually nonexistent. McC2 was also applied in a clinical diagnostic test for the detection of auto anti-nuclear antibodies in patient sera. McC2 was substituted, in a blind study, for a HRP-conjugated second antibody supplied with the test kit. All sera were tested both with the kit's second antibody and McC2. When using McC2, we obtained no false positive results whereas five false positives were obtained when using the kit's second antibody. However, one false negative result was obtained with the use of McC2 as a developing reagent while none were noted with the use of the kit's second antibody.(ABSTRACT TRUNCATED AT 250 WORDS)

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Role of Immunology in Defining Transmitter‐Specific Neurons

Cited by 6 publications

References 106 publications

Presynaptic Versus Postsynaptic Localization of μ and δ Opioid Receptors in Dorsal and Ventral Striatopallidal Pathways

Presynaptic Versus Postsynaptic Localization of μ and δ Opioid Receptors in Dorsal and Ventral Striatopallidal Pathways

Morphological characterization of substance P‐like immunoreactive glomeruli in the superficial dorsal horn of the rat spinal cord and trigeminal subnucleus caudalis: A quantitative study

The production of a ?universal developer? for the immunological detection of human IgG and its application in immunodiagnostics

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