Role of Immunohistochemical Markers in Surgical Margins of Patients with Head and Neck Carcinoma – A Systematic Review

Abstract

Objective(s):The aim and objective of this study was to systematically evaluate the prognostic role of immunohistochemical markers in surgical margins of patients with head and neck squamous cell carcinoma (HNSCC).Materials and Methods: MEDLINE/PubMed, Scopus and Cochrane library were searched for relative studies until December 2018. Retrospective and prospective original research studies published in English language assessing the prognostic value of immunohistochemical markers and disease-free survival in HNSCC and oral squamous cell carcinoma (OSCC) were included.Results: A total of eight studies were included comprising of 269 cases. The studies included here used eukaryotic initiation transcription factor 4E (eIF4E) in HNSCC patients; Matrix metalloproteinase (MMP), MMP2, MMP3, MMP9, Dentin Sialo-Phosphoprotein (DSPP), Bone Sialoprotein (BSP), Osteopontin (OPN), Beta-2-adrenergic receptors and E-cadherin in OSCC patients and p53 in HNSCC and OSCC. Among all the markers studied MMP9 had the highest accuracy at 80% followed by p53 (75%), DSPP (70%) and OPN (70%) while eIF4E (33.3%) had least accuracy. A study suggested that E-cadherin is the preferred marker over MMP9. Almost all the studies used Fisher's exact and Fisher-Freeman-Halton significance test. Only one study was at low risk of bias, three studies were at moderate risk of bias, three studies had serious risk of bias and in one study bias could not be calculated due to inadequate information. Conclusions:The study shows that immunohistochemical markers can significantly contribute to the field of head and neck carcinomas. Future efforts should concentrate on improving the antibody selection and its performance in the patients.

Comparison of Microwave Treatment and Pressure Cooker Methods for Antigen Retrieval Techniques in Immunohistochemistry

“…The extreme high temperatures may be expected to provide sufficient vitality to deliver the calcium particles and divalent metal cations from the pen like buildings that they form with proteins. A Number of related studies were reviewed [12][13][14][15][16][17]. The present investigation gives further help to the usefulness of HBAR and gives new data in order to change this 'kitchen' approach into a more standardized apparatus [18][19][20][21][22][23][24][25][26][27][28][29][30].…”

The prognostic role of immunohistochemical markers in surgical margins of Indian patients with oral squamous cell carcinoma- a retrospective study protocol

“…7 Immunohistochemical evaluation of negative surgical margins could embody a more reliable method to detect the MRC in them as genetic alteration goes ahead of the phenotypic changes in the epithelium. 8 To establish the dependable immunohistochemical marker, the IHC marker ought to be precise for tumor cells that could distinguish between normal cells and tumor cells. 9 Recent studies are centered on finding the IHC marker that may provide useful prognostic information and help in better clinical management of OSCC patients.…”