Role of IL28B genotyping in patients with hepatitis C virus-associated mixed cryoglobulinemia and response to PEG-IFN and ribavirin treatment

Boglione, Lucio; Cusato, Jessica; Allegra, Sarah; Castaldo, Giuseppe; Perri, Giovanni Di; D’Avolio, Antonio

doi:10.1007/s00705-015-2482-3

Cited by 10 publications

(7 citation statements)

References 38 publications

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“…[36] Another study showed that Patients with the TT/CC genotype for rs12979860/rs8099917 had a significant correlation with higher HCVRNA levels. [37] Our results did not confirm this paradoxical association, but it shows that as we expect a better response to treatment. Favorable polymorphisms of rs12979860 are associated with a lower base line viral load.…”

Section: Discussioncontrasting

confidence: 72%

A Tetra-Primer Amplification Refractory Mutation System–Polymerase Chain Reaction (T-ARMS-PCR) for Genotyping of rs8099917 & rs12979860 IL28B Polymorphisms and Its Correlation of Various Variables in Iranian HCV Patients

Bahari

Hashemi

Bahari

et al. 2020

JPRI

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Background: Selecting patients for new direct acting antiviral treatment of HCV has prompted a conflicting matter worldwide because of its high cost and limited availability. Genotyping of IL28B polymorphisms will aid clinical decision making for identifying priorities of urgent treatment in resource-limited countries. Objectives: The aim of the present study was to design a simple tetra-primer amplification refractory mutation system–polymerase chain reaction (T-ARMS-PCR) for genotyping of the rs8099917 and rs12979860 IL28B gene polymorphisms. Furthermore, we identify the correlation of variables such as gender, serum ALT level, histology of liver and baseline viral load with these polymorphisms. Patients and Methods: We efficiently designed a T-ARMS-PCR for detection of rs12979860 and rs8099917 IL28B gene polymorphisms. Using this method, we genotyped 148 hepatitis C patients. To ensure T-ARMS genotyping quality, we, regenotyped samples with the PCR- sequencing method. Results: Results of genotyping of rs12979860 and rs8099917 by T-ARMS PCR method were 100% concordant with the sequencing results. Among these 148 patients with chronic hepatitis C, the frequency of the rs12979860 CT, TT and CC genotypes was 72.3%, 14.2% and 13.5%, respectively and the frequency of the rs8099917 TT, GT and GG genotypes was 58.1%, 38.5% and 3.4%. Low frequency (2.7%) of association of two unfavourable homozygous genotypes (TT rs12979860 / GG rs809917) as well as 56.7% of association of 3 or 4 favorable alleles could explain good response of Iranians to HCV treatment with interferon-based regimens. About correlation of polymorphisms with different variables, only high viral load showed a statistically significant correlation to unfavorable genotype of TT rs12979860 ( p value = 0/05 ) and there was no correlation of serum ALT level, gender and histology of liver to IL28B genotypes. Conclusions: We report that rs8099917 polymorphisms could predict outcomes better than rs12979860 in Iranian HCV patients.

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Section: Discussioncontrasting

confidence: 72%

A Tetra-Primer Amplification Refractory Mutation System–Polymerase Chain Reaction (T-ARMS-PCR) for Genotyping of rs8099917 & rs12979860 IL28B Polymorphisms and Its Correlation of Various Variables in Iranian HCV Patients

Bahari

Hashemi

Bahari

et al. 2020

JPRI

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“…Boglione et al 20 found the combined assessment of both IL28B SNPs useful in MC; nevertheless, the Authors included a consistent group of Egyptian patients presenting with different distribution and predictive value of IL28B with respect to Caucasians.…”

Section: Discussionmentioning

confidence: 99%

“… 18 Nevertheless, a different distribution of IL28B genotypes has been reported by a recent study, even though this result may be dependent on the geographical origin of the enrolled patients. 20 …”

Section: Discussionmentioning

confidence: 99%

“…34 , 35 A further study reported that cryoglobulinemia is associated with low SVR rates in symptomatic patients, while asymptomatic MC presents with similar SVR rates than noncryoglobulinemic subjects. 20 It is conceivable that the discrepancies might be dependent on ethnic differences. 36 – 40 Moreover, this study confirms that IL28B SNPs are associated with SVR, irrespective of HCV genotype, in patients with no circulating cryoglobulins but not in those with presence of serum cryoglobulins.…”

Section: Discussionmentioning

confidence: 99%

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Combined Effects of 2 Interleukin 28B Polymorphisms on the Therapeutic Outcome of Hepatitis C Patients With Circulating Cryoglobulins

et al. 2015

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Chronic hepatitis C is commonly associated with extrahepatic manifestations. Cryoglobulins are observed in 40% to 60% of such patients and their presence seems to modify response to therapy. The new antivirals are greatly improving the sustained virological response (SVR); however, their high cost limits the use, leaving pegylated interferon plus ribavirin (PR) still the standard-of-care therapy worldwide. Since PR therapy is burdened with several side effects, pretreatment predictions of patients who are unlikely to respond to this regimen may avoid ineffective treatment. Variants of the interleukin-28B (IL28B) gene correlate with an SVR to PR, and combined IL28B polymorphisms may improve the prediction of treatment outcome.The potential role of both rs8099917 and rs12979860 IL28B single nucleotide polymorphisms (SNPs) combined with presence of cryoglobulins in predicting SVR to PR in hepatitis C virus (HCV)-chronically infected patients was analyzed in the present study.Single and combined IL28B SNPs (rs12979860 and rs8099917) were analyzed in 64 chronic HCV patients treated with PR showing circulating cryoglobulins and compared to 108 noncryoglobulinemic subjects to verify the predictive value on the SVR.The association of rs12979860CC or rs8099917TT with SVR was confirmed in the noncryoglobulinemic group but not in cryoglobulinemic patients. Moreover, the combined determination of both SNPs improved the prediction of SVR in noncryoglobulinemic patients but not in the cryoglobulinemic subgroup.We report that both single and combined determination of IL28B rs12979860 and rs8099917 SNPs in chronic HCV patients with circulating cryoglobulins treated with PR may have a reduced predictive value of SVR.

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“…Patients with CC or TT genotypes present a higher baseline viral load than CT/TT or TG/GG genotypes, despite a greater chance of viral clearance or response to PEG-IFN. Regarding this finding, in our previous published study we reported that the IL28B genotypes were related both to baseline viremia and to the presence or absence of mixed cryoglobulinemia [5]. Patients with the TT/CC genotype for rs12979860/rs8099917 showed a significant correlation with higher HCV-RNA levels, the presence of mixed cryoglobulinemia and insulin resistance.…”

Section: Figmentioning

confidence: 91%

The Role of IL28B Genotype in HCV-RNA Baseline Levels

Boglione

Cusato²,

Perri³

et al. 2016

Intervirology

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Role of IL28B genotyping in patients with hepatitis C virus-associated mixed cryoglobulinemia and response to PEG-IFN and ribavirin treatment

Cited by 10 publications

References 38 publications

A Tetra-Primer Amplification Refractory Mutation System–Polymerase Chain Reaction (T-ARMS-PCR) for Genotyping of rs8099917 & rs12979860 IL28B Polymorphisms and Its Correlation of Various Variables in Iranian HCV Patients

A Tetra-Primer Amplification Refractory Mutation System–Polymerase Chain Reaction (T-ARMS-PCR) for Genotyping of rs8099917 & rs12979860 IL28B Polymorphisms and Its Correlation of Various Variables in Iranian HCV Patients

Combined Effects of 2 Interleukin 28B Polymorphisms on the Therapeutic Outcome of Hepatitis C Patients With Circulating Cryoglobulins

The Role of IL28B Genotype in HCV-RNA Baseline Levels

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