Role of<i>Fabp7</i>, a Downstream Gene of Pax6, in the Maintenance of Neuroepithelial Cells during Early Embryonic Development of the Rat Cortex

Arai, Yoko; Funatsu, Nobuo; Numayama-Tsuruta, Keiko; Nomura, Tadashi; Nakamura, Shun; Osumi, Noriko

doi:10.1523/jneurosci.2512-05.2005

Cited by 158 publications

(149 citation statements)

References 61 publications

(71 reference statements)

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“…Pax6 is a multifunctional protein that regulates the proliferation and differentiation of neural stem/progenitor cells (NSCs) in the CNS by regulating the expression of several downstream genes, including Fabp7, Ngn2, and Wnt7b [16,[34][35][36][37]. Pax6 is also expressed in astrocytes in late embryogenesis and postnatally, and deleting Pax6 causes cells to retain their NSC-like properties and inhibits astrocyte maturation [28].…”

Section: Discussionmentioning

confidence: 99%

Horizontal Basal Cell-Specific Deletion ofPax6Impedes Recovery of the Olfactory Neuroepithelium Following Severe Injury

Suzuki

Sakurai

Yamazaki

et al. 2015

Stem Cells and Development

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In the mammalian olfactory epithelium (OE), olfactory receptor neurons (ORNs) are continuously regenerated throughout the animal's lifetime. Horizontal basal cells (HBCs) in the OE express the epithelial marker keratin 5 (K5) and the stem cell marker Pax6 and are considered relatively quiescent tissue stem cells in the OE. Pax6 is a key regulator of several developmental processes in the central nervous system and in sensory organs. Although Pax6 is expressed in the OE, its precise role remains unknown, particularly with respect to stem celllike HBCs. To investigate the function of Pax6 in the developmental and regenerative processes in the OE, we generated conditional Pax6-knockout mice carrying a loxP-floxed Pax6 gene. Homozygous Pax6-floxed mice were crossed with K5-Cre transgenic mice to generate HBC-specific Pax6-knockout (Pax6-cKO) mice. We confirmed that the deletion of Pax6 expression in HBCs was sufficiently achieved in zone 1 of the OE in Pax6-cKO mice 3 days after methimazole-induced severe damage. In this condition, regeneration of the OE was dramatically impaired; both OE thickness and the number of ORNs were significantly decreased in the regenerated OE of Pax6-cKO mice. These results suggest that Pax6 expression is essential for HBCs to differentiate into neuronal cells during the regeneration process following severe injury.

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Section: Discussionmentioning

confidence: 99%

Horizontal Basal Cell-Specific Deletion ofPax6Impedes Recovery of the Olfactory Neuroepithelium Following Severe Injury

Suzuki

Sakurai

Yamazaki

et al. 2015

Stem Cells and Development

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“…Among various FABPs, brain (B-), epidermal (E-), and heart (H-) type FABPs are expressed in brain (Owada et al, 1996). B-FABP knockdown by small interfering RNA impairs cell proliferation and promotes neuronal differentiation in cortical neuroepithelial cells (Arai et al, 2005). B-FABP knock-out (KO) mice show abnormalities in emotional behavior (Owada et al, 2006), decreased neurogenesis in the dentate gyrus, and impaired prepulse inhibition (Watanabe et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Heart-Type Fatty Acid Binding Protein Regulates Dopamine D₂Receptor Function in Mouse Brain

Shioda¹,

Yamamoto²,

Watanabe³

et al. 2010

J. Neurosci.

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“…Various inhibitors can be applied to the culture medium to examine signaling pathways involved in axis formation and cell cycle during development 10,11 . Furthermore, we can simply introduce expression plasmids and siRNA into developing brains of cultured embryos by electroporation to analyze gene functions 12,13,14 . We also investigated behavior of neuroepithelial cells labeled with fluorescent protein in organ culture or slice culture following WEC (see ref.…”

Section: Discussionmentioning

confidence: 99%

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

Takahashi

Osumi

2010

JoVE

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Whole embryo culture (WEC) technique has been developed in 1950's by New and his colleagues, and applied for developmental biology 1 . Although development and growth of mammalian embryos are critically dependent on the function of the placenta, WEC technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages during embryonic day (E) 6.5-E12.5 in the mouse or E8.5-E14.5 in the rat 2, 3, 4 . In WEC, we can directly target desired areas of embryos using fine glass capillaries because embryos can be manipulated under the microscope. Therefore, rodent WEC is very useful technique when we want to study dynamic developmental processes of postimplanted mammalian embryos. Up to date, several types of WEC systems have been developed 1 . Among those, the rotator-type bottle culture system is most popular and suitable for long-term culture of embryos at midgestation, i.e., after E9.5 and E11.5 in the mouse and rat, respectively 1 . In this video protocol, we demonstrate our standard procedures of rat WEC after E12.5 using a refined model of the original rotator system, which was designed by New and Cockroft 5, 6 , and introduce various applications of WEC technique for studies in mammalian developmental biology.

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Role ofFabp7, a Downstream Gene of Pax6, in the Maintenance of Neuroepithelial Cells during Early Embryonic Development of the Rat Cortex

Cited by 158 publications

References 61 publications

Horizontal Basal Cell-Specific Deletion ofPax6Impedes Recovery of the Olfactory Neuroepithelium Following Severe Injury

Horizontal Basal Cell-Specific Deletion ofPax6Impedes Recovery of the Olfactory Neuroepithelium Following Severe Injury

Heart-Type Fatty Acid Binding Protein Regulates Dopamine D₂Receptor Function in Mouse Brain

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

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Role ofFabp7, a Downstream Gene of Pax6, in the Maintenance of Neuroepithelial Cells during Early Embryonic Development of the Rat Cortex

Cited by 158 publications

References 61 publications

Horizontal Basal Cell-Specific Deletion ofPax6Impedes Recovery of the Olfactory Neuroepithelium Following Severe Injury

Horizontal Basal Cell-Specific Deletion ofPax6Impedes Recovery of the Olfactory Neuroepithelium Following Severe Injury

Heart-Type Fatty Acid Binding Protein Regulates Dopamine D2Receptor Function in Mouse Brain

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

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Heart-Type Fatty Acid Binding Protein Regulates Dopamine D₂Receptor Function in Mouse Brain