Role ofBlepharis maderaspatensisandAmmannia bacciferaplant extracts onin vitrooxygen radical scavenging, secretion of gastric fluid and gastroprotection on ulcer induced rats

Rajasekaran, A.; Sivakumar, Vellaichamy; Darlinquine, Sabarimuthu

doi:10.3109/13880209.2012.657305

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…The curative properties of Blepharis species paved the way for researchers to explore their therapeutic properties. Herein, several pharmacological properties for other plant species from the genus Blepharis have been reported, such as anti-inflammatory and anti-nociceptive properties [6], wound healing activities [7], and gastroprotective [8] effects of B. maderaspatensis (L.) B.Heyne ex Roth. Moreover, other studies reported antioxidant activity for B. maderaspatensis and B. molluginifolia Pers.…”

Section: Introductionmentioning

confidence: 99%

Phenolic Compounds from the Aerial Parts of Blepharis linariifolia Pers. and Their Free Radical Scavenging and Enzyme Inhibitory Activities

et al. 2019

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Background: Blepharis linariifolia Pers. (Family: Acanthaceae) is used in traditional medicines as a general tonic and for the treatment of various health problems in Sudan. The main aim of this study was to isolate and identify the major chemical constituents from the aerial parts of B. linariifolia and evaluate their bioactivities. Methods: The dried aerial parts of the plant were extracted successively with 100% acetone and 50% acetone, and thereafter the combined extract was subjected to repeated column chromatography to isolate the main components. Free radical scavenging activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical method, and in vitro enzyme inhibitory activities against α-glucosidase, pancreatic lipase, and mushroom tyrosinase were evaluated. Results: From the detailed chemical analysis, verbascoside (1), vanillic acid (2), apigenin (3), and 6″-O-p-coumaroylprunin (4), were isolated and their structures were identified on the basis of their nuclear magnetic resonance (NMR) spectral data. Among the isolated compounds, verbascoside (1) showed the most potent free radical scavenging activity (IC50 = 22.03 ± 0.04 μM). Apigenin (3) and 6″-O-p-coumaroylprunin (4) showed promising inhibitory activities against all tested enzymes. Apigenin (3) showed the most potent inhibitory activity against α-glucosidase and tyrosinase (IC50 = 34.73 ± 1.78 μM and 23.14 ± 1.83 μM, respectively), whereas 6″-O-p-coumaroylprunin (4) showed potent inhibition for lipase (IC50 = 2.25 ± 0.17 μM). Conclusions: Four phenolic compounds were isolated and identified from B. linariifolia acetone extract, which are reported for the first time from this plant. All compounds showed good DPPH free radical scavenging activities, with verbascoside (1) being the most potent. Apigenin (3) was the most active as α-glucosidase and mushroom tyrosinase inhibitor, while 6″-O-p-coumaroylprunin (4) showed potent inhibitory activity for pancreatic lipase.

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Section: Introductionmentioning

confidence: 99%

Phenolic Compounds from the Aerial Parts of Blepharis linariifolia Pers. and Their Free Radical Scavenging and Enzyme Inhibitory Activities

et al. 2019

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“…The lowest effective dose of chrysin, 10 mg/kg, was used in the subsequent experiments because there was no significant difference among the tested doses. Ethanol can easily penetrate the gastric mucosa, and the lesions observed in the gastric mucosa can be attributed to the production of reactive oxygen species, rupture of endogenous mucus, secretion of gastric acid [13], damage to the gastric mucosa related to hemorrhagic lesions, apoptosis, induction of lipid peroxidation and oxidative stress, and reduction in reduced glutathione (GSH) and prostaglandin levels [14]. The cellular damage increases neutrophil infiltration in the gastric tissue, resulting in the generation of free radicals that consequently aggravate the lesions [15].…”

Section: Resultsmentioning

confidence: 99%

Chrysin Modulates Genes Related to Inflammation, Tissue Remodeling, and Cell Proliferation in the Gastric Ulcer Healing

Fagundes

Piffer

Périco

et al. 2020

IJMS

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Chrysin exhibits anti-inflammatory and antioxidant activities. Here, the gastroprotective effect of chrysin was investigated in mouse models of gastric ulcer induced by absolute ethanol, acetic acid, and ischemia-reperfusion injury. The gastric-healing effect was evaluated at 7 and 14 days after treatment; the mechanism of action was verified using the expression of metalloproteinase 2 (MMP-2) and 9 (MMP-9), caspase-3, cyclooxygenase 1 (COX-1) and 2 (COX-2), epidermal growth factor (EGF), and interleukin-10. Chrysin (10 mg/kg) inhibited macroscopic lesions and increased catalase activity in the mouse model established using absolute ethanol. It ameliorated the gastric ulcer caused by acetic acid by improving the expression of inflammatory genes such as COX-2, inhibiting negative remodeling promoted by MMP-9, increasing cell proliferation effect via EGF, and reducing cellular apoptosis by modulating caspase-3. A faster healing effect was evident in the first 7 days of treatment compared to 14 days of treatment, indicating the pharmacological potential of chrysin. Overall, these results demonstrate the potent effect of chrysin in the gastrointestinal tract and elucidate the genes involved in the healing of gastric ulcers. Moreover, an increase in the levels of gastric mucosa defensive factors is involved in the activity of chrysin in the gastric mucosa.

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“…It can quickly penetrate the gastric mucosal layer in a short time, causing damage to the membrane and leading to tissue erosion (Sidahmed et al, 2013), endogenous mucus rupture, and increased gastric acid secretion. These effects result in the development of hemorrhagic lesions and gastric mucosal injury (Rajasekaran et al, 2012). An ethanolinduced peptic ulcer disease murine model has been widely used in the study of the mechanism of anti-ulcer drugs (Zhang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture ameliorates peptic ulcer disease in association with gastroduodenal microbiota modulation in mice

Feng²,

Tuo³

et al. 2022

Front. Cell. Infect. Microbiol.

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Peptic ulcer disease (PUD) is a common disease and frequently encountered in the clinic. Accumulating evidence suggests that PUD is associated with the gastrointestinal microbiota. Electroacupuncture (EA) is an improved version of acupuncture, which can improve the clinical effect by increasing the stimulation and delivering appropriate electrical pulses to needles. This method has been widely used in the treatment of peptic ulcer disease. However, its effect on gastrointestinal microbiota remains unclear. Therefore, in the present study, the ameliorative effect of EA was evaluated on the gastroduodenal mucosa, and the regulatory effect of the gastroduodenal microbiota was assessed in PUD mice. A total of 48 male Kun Ming mice were randomly divided into the following groups: normal control group (NC), PUD model group (PUD), Shousanli group (LI10), and Zusanli group (ST36) (n=12). The mice in groups LI10 and ST36 were treated with EA at LI10 and ST36, respectively. This intervention was continued for 7 days. Subsequently, we evaluated the morphological changes in the gastric and duodenal mucosa, and specific indices were measured, including the contents of serum dopamine (DA), the trefoil factor (TFF), and the vasoactive intestinal peptide (VIP). In addition, the gastric and duodenal microbiota were assessed via 16S ribosomal DNA sequencing. The results indicated that EA at LI10 or ST36 significantly reduced the injury of the gastroduodenal mucosa in PUD mice. The gastric microbial community structure of the groups LI10 and ST36 was similar to that of the NC group following comparison with the microbial community structure of the PUD model group. Moreover, the abundance of Firmicutes in the stomach was decreased, whereas that of Bacteroidetes was increased, and the abundance of Firmicutes in the duodenum was decreased. Furthermore, the microbial diversity and richness of the gastric microbiota in group LI10 were also significantly increased, and the serum dopamine and trefoil factor levels in group ST36 were significantly increased. Therefore, it is suggested that EA ameliorating PUD is in association with improving the levels of DA and TFF and regulating the relative abundances of Firmicutes and Bacteroidetes in the gastric microbiota.

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Role ofBlepharis maderaspatensisandAmmannia bacciferaplant extracts onin vitrooxygen radical scavenging, secretion of gastric fluid and gastroprotection on ulcer induced rats

Cited by 12 publications

References 22 publications

Phenolic Compounds from the Aerial Parts of Blepharis linariifolia Pers. and Their Free Radical Scavenging and Enzyme Inhibitory Activities

Phenolic Compounds from the Aerial Parts of Blepharis linariifolia Pers. and Their Free Radical Scavenging and Enzyme Inhibitory Activities

Chrysin Modulates Genes Related to Inflammation, Tissue Remodeling, and Cell Proliferation in the Gastric Ulcer Healing

Electroacupuncture ameliorates peptic ulcer disease in association with gastroduodenal microbiota modulation in mice

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