Role of hydroxyapatite nanoparticle size in bone cell proliferation

Cai, Yurong; Liu, Yukan; Yan, Wei; Hu, Qinghong; Tao, Jinhui; Zhang, Ming; Shi, Zhijun; Tang, Ruikang

doi:10.1039/b705129h

Cited by 367 publications

(308 citation statements)

References 45 publications

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“…NanoHA possesses a significantly higher surface area, porosity and densification, which may improve its mechanical properties under load, shows solubility in vivo and the capacity to penetrate cell membranes [25]. Also, nanoscale topography have a positive effect on osteoblastic proliferation and differentiation, and the nano-crystals allow a constant regeneration of bone, resulting in improved biocompatibility and osteointegration [26]. Some authors have addressed the capacity of blending nanoHA with antimicrobial agents (e.g., amoxicillin, erythromycin, minocycline, zinc oxid, cobalt) [8,[27][28][29] or materials (e.g., hydroxide, chitosan) [30,31] for the treatment of infections related to biomedical devices and implants.…”

Section: Introductionmentioning

confidence: 99%

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Barros

Grenho

Fernandes

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Nanohydroxyapatite possesses exceptional biocompatibility and bioactivity regarding bone cells and tis-sues, justifying its use as a coating material or as a bone substitute. Unfortunately, this feature may also encourage bacterial adhesion and biofilm formation. Surface functionalization with antimicrobials is a promising strategy to reduce the likelihood of bacterial infestation and colonization on medical devices. Chlorhexidine digluconate is a common and effective antimicrobial agent used for a wide range of medical applications. The purpose of this work was the development of a nanoHA biomaterial loaded with CHX to prevent surface bacterial accumulation and, simultaneously, with good cytocompatibility, for application in the medical field. CHX (5-1500 mg/L) was loaded onto nanoHA discs and the materials were evaluated for CHX adsorption and release profile, physic-chemical features, antibacterial activity against Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis, and cytocompatibility toward L929 fibroblasts. Results showed that the adsorption of CHX on nanoHA surface occurred by electrostatic inter-actions between the cationic group of CHX and the phosphate group of nanoHA. The release of CHX from CHX-loaded nanoHA showed a fast initial rate followed by a slower kinetics release, due to constraints caused by dilution and diffusion-limiting processes. NanoHA.50 to nanoHA.1500 showed strong anti-sessile activity, inhibiting bacterial adhesion and the biofilm formation. CHX-nanoHA caused a dose-and time-dependent inhibitory effect on the proliferation of fibroblasts for nanoHA.100 to nanoHA.1500. Cellular behavior on 2 nanoHA.5 and nanoHA.50 was similar to control. Therefore, CHX-loaded nanoHA surfaces appear as a promising alternative to prevention of devices-related infections.

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Section: Introductionmentioning

confidence: 99%

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Barros

Grenho

Fernandes

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…54 Furthermore, 2Fe-SeHA material had the lowest crystallite size (about 48 nm) ( Table II), suggesting that 2Fe-SeHA nanoparticles might have penetrated into the cell membranes and inhibited the cell growth. 55 To evaluate how hFOB cells responded to different surfaces of Fe-SeHA materials, the morphology of cells on these materials was studied by SEM examinations. The morphology of hFOB cells on Fe-SeHA materials discs after 3 days of incubation can be seen in SEM images Figure 9(a-d).…”

Section: Resultsmentioning

confidence: 99%

“…54 Another reason would be that cells might have been disturbed due to probable penetration of these nanoparticles into the cells. 55 Tissue nonspecific alkaline phosphatase (ALP) is the natural enzyme produced by osteoblasts cells during osteogenesis. To investigate the early stage of stem cell differentiation, ALP expression and intracellular calcium deposition are considered as an early osteoblastic marker of osteogenic differentiation.…”

Section: Resultsmentioning

confidence: 99%

Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

et al. 2017

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Dual ions substituted hydroxyapatite (HA) received attention from scientists and researchers in the biomedical field owing to their excellent biological properties. This paper presents a novel biomaterial, which holds potential for bone tissue applications. Herein, we have successfully incorporated ferric (Fe 31 )/selenate (SeO 22 4 ) ions into the HA structure (Ca 10-x-y Fe y (PO 4 ) 6-x (SeO 4 ) x (OH) 2-x-y O y ) (Fe-SeHA) through a microwave refluxing process. The Fe-SeHA materials were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and field emission scanning electron microscopy (FESEM). XRD and FTIR analyses revealed that Fe-SeHA samples were phase pure at 9008C. FESEM images showed that formation of rod-like shaped particles was inhibited dramatically with increasing Fe 31 amount. The Vickers hardness (HV) test showed that hardness values increased with increasing Fe 31 concentrations. Optical spectra of Fe-SeHA materials contained broadband over (200-600) nm. In vitro degradation and bioactivity tests were conducted in simulated body fluid (SBF). The incorporation of Fe 31 /SeO 22 4 ions into the HA structure resulted in a remarkably higher degradation rate along with intense growth of apatite granules on the surface of the Fe-SeHA discs with Ca/P ratio of 1.35-1.47. In vitro protein adsorption assay was conducted in fetal bovine serum (FBS) and it was observed that the adsorption of serum proteins on Fe-SeHA samples significantly increased with increasing Fe 31 concentration. In vitro cytotoxicity tests were performed with human fetal osteoblast (hFOB)

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“…On the one hand, hydroxyapatite and titania nanoparticles of up to 40 nm diameter decreased osteoblastic cell proliferation and viability, respectively. 99,116 On the other hand, in another study, only hydroxyapatite nanoparticles with a diameter of 20 nm enhanced cell growth of osteoblast-like cells compared with larger particles with a diameter of 80 nm. 117 In addition to particle uptake by osteoblasts and potential effects on proliferation and viability, the consequences of nanoparticle presence on the differentiation and mineralization of osteoblastic cells were assessed in a variety of studies with a wide range of particles.…”

Section: Osteoblastsmentioning

confidence: 99%

“…98 MSC proliferation was affected in a sizedependent manner by the exposure of these cells to calcium phosphate nanoparticles, with larger particles being more harmful. 99 The same research group further investigated a potential dependency on particle concentration and their form of appearance. It was observed that with increasing concentration as well as with amorphous particles in contrast to crystalline calcium phosphate particles, osteogenic cell differentiation and matrix mineralization decreased.…”

mentioning

confidence: 99%

Nanoparticles and their potential for application in bone

Tautzenberger¹,

Kovtun²,

Ignatius³

2012

IJN

161

137

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Abstract:Biomaterials are commonly applied in regenerative therapy and tissue engineering in bone, and have been substantially refined in recent years. Thereby, research approaches focus more and more on nanoparticles, which have great potential for a variety of applications. Generally, nanoparticles interact distinctively with bone cells and tissue, depending on their composition, size, and shape. Therefore, detailed analyses of nanoparticle effects on cellular functions have been performed to select the most suitable candidates for supporting bone regeneration. This review will highlight potential nanoparticle applications in bone, focusing on cell labeling as well as drug and gene delivery. Labeling, eg, of mesenchymal stem cells, which display exceptional regenerative potential, makes monitoring and evaluation of cell therapy approaches possible. By including bioactive molecules in nanoparticles, locally and temporally controlled support of tissue regeneration is feasible, eg, to directly influence osteoblast differentiation or excessive osteoclast behavior. In addition, the delivery of genetic material with nanoparticulate carriers offers the possibility of overcoming certain disadvantages of standard protein delivery approaches, such as aggregation in the bloodstream during systemic therapy. Moreover, nanoparticles are already clinically applied in cancer treatment. Thus, corresponding efforts could lead to new therapeutic strategies to improve bone regeneration or to treat bone disorders.

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Role of hydroxyapatite nanoparticle size in bone cell proliferation

Cited by 367 publications

References 45 publications

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications

Nanoparticles and their potential for application in bone

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Role of hydroxyapatite nanoparticle size in bone cell proliferation

Cited by 367 publications

References 45 publications

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Fe3+/− dual doped nano hydroxyapatite: A novel material for biomedical applications

Nanoparticles and their potential for application in bone

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Fe³⁺/⁻ dual doped nano hydroxyapatite: A novel material for biomedical applications