Role of hydroperoxide lyase in white-backed planthopper (Sogatella furciferaHorváth)-induced resistance to bacterial blight in rice,Oryza sativaL.

Abstract

SUMMARYA pre-infestation of the white-backed planthopper (WBPH), Sogatella furcifera Horvá th, conferred resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) in rice (Oryza sativa L.) under both laboratory and field conditions. The infestation of another planthopper species, the brown planthopper (BPH) Nilaparvata lugens Stå l, did not significantly reduce the incidence of bacterial blight symptoms. A large-scale screening using a rice DNA microarray and quantitative RT-PCR revealed that WBPH infestation caused the upregulation of more defence-related genes than did BPH infestation. Hydroperoxide lyase 2 (OsHPL2), an enzyme for producing C 6 volatiles, was upregulated by WBPH infestation, but not by BPH infestation. One C 6 volatile, (E)-2-hexenal, accumulated in rice after WBPH infestation, but not after BPH infestation. A direct application of (E)-2-hexenal to a liquid culture of Xoo inhibited the growth of the bacterium. Furthermore, a vapour treatment of rice plants with (E)-2-hexenal induced resistance to bacterial blight. OsHPL2-overexpressing transgenic rice plants exhibited increased resistance to bacterial blight. Based on these data, we conclude that OsHPL2 and its derived (E)-2-hexenal play some role in WBPH-induced resistance in rice.

Accumulation of salicylic acid, jasmonic acid and phytoalexins in rice, Oryza sativa, infested by the white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)

“…The expression of the other antimicrobial genes, such as OsPR-1.1, OsPR-1.3, EL2 and PBZ1, by the method of Northern hybridization, and also the induction of four kinds of PR proteins, PR-1, 2, 3 and 5 by the method of Western blotting, were also confirmed in rice infested by S. furcifera (Kanno et al, unpublished data). Recently, Gomi et al (2010) reported that OsHPL2 and its derived (E)-2-hexenal play some role in S. furcifera-induced resistance to X. oryzae pv. oryzae in rice.…”

“…We subsequently H. Kanno Laboratory of Applied Entomology, Kyushu Okinawa National Agricultural Research Center, Kohshi, Kumamoto 861-1192, Japan confirmed the induced resistance to the bacterial leaf blight, Xanthomonas oryzae pv. oryzae (Ishiyama), in rice infested by S. furcifera (Gomi et al 2010).…”

Multiple roles of plant volatiles in jasmonate-induced defense response in rice

“…[12][13][14][15] In addition to the function as a signaling molecule, some volatile compounds have antimicrobial activities against plant pathogens. [16][17][18][19][20][21] When hydroperoxide lyase, which is a key enzyme to produce the C 6 and C 9 volatiles, was overexpressed in rice and Arabidopsis, these plants exhibited increased resistance against pathogens. 21,22 Furthermore, some defense-related genes were upregulated by vapor treatment with a C 6 volatile, (E)-2-hexenal, in rice and Arabidopsis.…”

“…[16][17][18][19][20][21] When hydroperoxide lyase, which is a key enzyme to produce the C 6 and C 9 volatiles, was overexpressed in rice and Arabidopsis, these plants exhibited increased resistance against pathogens. 21,22 Furthermore, some defense-related genes were upregulated by vapor treatment with a C 6 volatile, (E)-2-hexenal, in rice and Arabidopsis. 21,23 Among terpenoids, monoterpenes and sesquiterpenes are one of the universal volatile components of plant.…”

“…21,22 Furthermore, some defense-related genes were upregulated by vapor treatment with a C 6 volatile, (E)-2-hexenal, in rice and Arabidopsis. 21,23 Among terpenoids, monoterpenes and sesquiterpenes are one of the universal volatile components of plant. These volatile terpenoids are emitted from damaged plant tissues by attack of herbivores.…”