Role of hyaluronidase in the regulation of hemopoiesis

Abstract

We evaluated the possibility of hemopoiesis stimulation with hyaluronidase. The response of the blood system depended on the dose of this enzyme. Functional activity of hemopoietic precursor cells increased under the influence of hyaluronidase in a dose of 20 arb. units, which was accompanied by an increase in the secretion of hemopoietins by adherent myelokaryocytes and elevation of hemopoietic activity in blood plasma. The number of erythrokaryocytes and mature neutrophilic granulocytes in the bone marrow, as well as the count of reticulocytes and neutrophils in the peripheral blood increased under these conditions. Administration of hyaluronidase in a high dose (100 arb. units) led to uncoupling of proliferation and differentiation of hemopoietic precursors and produced no considerably changes in the blood.

Effects of granulocyte colony-stimulating factor and hyaluronidase on the formation of blood system reactions

“…units/mouse stimulated granulomonocyto-and erythropoiesis. These results coincided with the previously observed hematotropic effects of this enzyme [6]. Treatment with hyaluronidase in the specified dose increased the content of reticulocytes, stab and segmented neutrophils in the peripheral blood and the content of erythrokaryocytes and mature neutrophilic granulocytes in the hemopoietic tissue (Fig.…”

“…3). These changes associated with pronounced destruction of hyaluronic acid and chondroitin sulfate of the extracellular matrix [6,12] were observed after administration of hyaluroni- dase alone (which confirmed our previous results [6]) and in combination with G-CSF.…”

“…These findings and published data [2,6,9] attest to an important role of hyaluronic acid and hyaluronidase in the regulation of hemopoiesis, in particular under conditions of G-CSSF treatment. The regularities the formation of hemopoietic shifts are largely determined by the degree of hydrolysis of hyaluronic acid and the proportion between its different molecular forms in situ [6,11,12].…”

“…units/mouse) more markedly increased the content of reticulocytes in the peripheral blood compared to the corresponding values in animals receiving hyaluronidase alone (by 58.6% on day 8) and more drastically elevated the count of stab neutrophils compared to mice receiving G-CSF alone (by 92.3% on day 8). These changes were paralleled by a sharp decrease in the count of circulating segmented neutrophils, which was probably related to their migration into peripheral tissues [5,6]. This probably resulted from increased permeability of tissue barriers under the effect of hyaluronidase [12] and increased functional activity of neutrophils under the effect of G-CSF [5,8].…”

“…At the same time, the processes of hemopoiesis are largely determined by the state of extracellular matrix of the hemopoietic tissue, in particular, by hyaluronic acid in situ constituting up to 40% of all glycosaminoglycans [9,11,12]. Previous experiments showed [2,3,6] that hyaluronidase, the enzyme cleaving hyaluronic acid to polymers with different molecular weight and various effects on the function of cell elements [12], can modulate the state of the blood system. At the same time, the mechanisms of summary effects of individual cytokines and components of the extracellular matrix to hemopoiesis (i.e.…”