Role of Host and Bacterial Factors in Modulating Staphylococcal Adhesion to Implanted Polymer Surfaces

Vaudaux, Pierre; Yasuda, Hiroshi; Velazco, Maria Inés; Huggler, Elzbieta; Ratti, I.; Waldvogel, Francis; Lew, Daniel Pablo; Proctor, Richard A.

doi:10.1177/088532829000500204

Cited by 51 publications

(36 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Data (shown in figure 5) demonstrated that multiple S. aureus-binding regions were still expressed after limited proteolytic attack by different, nonoverlapping fragments of fibronectin. These in vitro data agree with previous observations [16,41] showing the presence of secondary S. aureus-binding region(s) of fibronectin, contributing to bacterial adherence, in addition to the initially described S. aureus-binding domain located in the 27-kDa amino-terminal segment [15,16,18,41). Whereas we found this secondary binding site in a mixture of 190-and 200-kDa tryptic fragments of fibronectin, which excluded the presence of the amino-terminal domain of the protein, a recent report has localized this site within the heparin-binding domain of fibronectin [46].…”

Section: Discussionsupporting

confidence: 94%

“…In contrast to fibrinogen, fragmented fibronectin could still adequately promote S. aureus Cowan I adherence [16,41]. Previous reports confirmed that the amino-terminal 27-kDa tryptic fragment bound specifically to S. aureus in suspension [15] or after adsorption on surfaces [16,41]. We also found a significant adherence-promoting activity in a mixture of 190-and 200-kDa tryptic fragments lacking both the amino-and carboxy-terminal ends of fibronectin [27,28].…”

Section: Selective Inactivation Ofadherence-promoting Activity Offibrsupporting

confidence: 59%

“…Finally, each of the 6 bacteremic isolates of S. aureus showed an extensive loss of adherence-promoting activity, similar to that of strain Cowan I, when tested on PUR-V cannulas coated with fragment D) versus native fibrinogen. In contrast to fibrinogen, fragmented fibronectin could still adequately promote S. aureus Cowan I adherence [16,41]. Previous reports confirmed that the amino-terminal 27-kDa tryptic fragment bound specifically to S. aureus in suspension [15] or after adsorption on surfaces [16,41].…”

Section: Selective Inactivation Ofadherence-promoting Activity Offibrmentioning

confidence: 99%

See 2 more Smart Citations

Fibronectin Is More Active than Fibrin or Fibrinogen in Promoting Staphylococcus aureus Adherence to Inserted Intravascular Catheters

Vaudaux¹,

Pittet²,

Haeberli³

et al. 1993

Journal of Infectious Diseases

Self Cite

136

View full text Add to dashboard Cite

To further define the role offibrin(ogen) and fibronectin in Staphylococcus aureusadherence to central venous catheters, the amount, chemical integrity, and biologic activity of these proteins adsorbed on lines inserted in hospitalized patients were prospectively studied. Polyurethane cannulas promoted a significantly lower adherence of S. aureusthan polyvinyl chloride (P < .01) or Hickman (P < .001) cannulas and contained the lowest amount of immunologically assayed fibronectin but not offibrin(ogen). Fibrinogen showed an extensive loss of adherence-promoting activity on inserted cannulas, which was related to its proteolytic breakdown, as detected by SDS-PAGE and immunoblots with antifibrinogen antibodies and confirmed by in vitro studies with purified protein fragments. In contrast, either intact or fragmented fibronectin, although present in much lower amounts than fibrin(ogen), could actively promote S. aureus adherence onto intravenous catheters.Microbial colonization of intravascular devices is an important source of nosocomial infection and sepsis [1,2]. Such device-related infections are frequently due to staphylococci and represent a major risk to the patients receiving intravenous (iv) therapy [I, 2]. Several factors may explain the frequent colonization of iv devices by Staphylococcus aureus, Staphylococcus epidermidis, or other species ofcoagulase-negative staphylococci: First, the presence ofa transcutaneous cannula wound allowing skin microorganisms to migrate across the skin barrier [1,2]; second, the microbial production of mucoid exopolymeric substances enhancing the stickiness of colonizing organisms [3][4][5][6]; and third, the presence of plasma proteins adsorbed on iv devices, which might selectively promote staphylococcal attachment to their surfaces [7][8][9][10].We have reported that plasma proteins deposited on various categories of iv catheters removed from patients played a significant role in staphylococcal adherence [11]. Clinical isolates of S. aureus were more strongly promoted for adherence by cannula-adsorbed plasma proteins than clinical isolates of S. epidermidis [11]. This observation was explained in part by the more frequent occurrence ofclinical [8,12,13] 1993;167:633-41 of S. epidermidis on surface determinants recognizing some major plasma or matrix proteins, such as fibronectin [8,[10][11][12][14][15][16][17][18], fibrinogen [9,16,[19][20][21], collagen [13, 17], laminin [8, 17, 22], or vitronectin [23]. Each of these individual proteins is known to promote staphylococcal adherence when adsorbed in vitro on polymeric surfaces [8][9][10]. In our in vivo study, we also found that on cannulas inserted in patients, most of the adherence-promoting activity was mediated via surface-bound fibronectin and fibrinogen or fibrin [11]. However, the respective roles of each of these protein components in staphylococcal adherence could not be precisely evaluated [11].The objectives ofthis study were to quantify the respective content of surface-bound fibronectin versus fibrinoge...

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Selective Inactivation Ofadherence-promoting Activity Offibrsupporting

confidence: 59%

Section: Selective Inactivation Ofadherence-promoting Activity Offibrmentioning

confidence: 99%

See 1 more Smart Citation

Fibronectin Is More Active than Fibrin or Fibrinogen in Promoting Staphylococcus aureus Adherence to Inserted Intravascular Catheters

Vaudaux¹,

Pittet²,

Haeberli³

et al. 1993

Journal of Infectious Diseases

Self Cite

136

View full text Add to dashboard Cite

show abstract

“…The microtiter plate method was used to evaluate and quantify the attachment ability of these strains. Microbial cell attachment to surfaces and the formation of multicellular communities is a key step in infection, and this characteristic is considered to be a virulence factor for the organism [30]. Using the microtiter plate method, all of the MRSA strains studied were found to produce biofilm except for one isolate; this rate was much lower (60%) in MSSA isolates.…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation and antimicrobial resistance in methicillin-resistant Staphylococcus aureus isolated from burn patients, Iran

Moghadam

Pourmand

Aminharati

2014

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Burns are the most serious forms of trauma and a major cause of mortality worldwide. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common pathogens of burn wound infections; treatment has faced serious problems due to antibiotic resistance in these strains. Biofilm formation, which increases antibiotic resistance capabilities and is considered to be a virulence factor, also causes treatment failure and recurrent staphylococcal infections in burn patients. Methodology: A total of 135 pus/wound swabs were collected; S. aureus was identified by confirmatory tests. The icaA/D and mecA genes were detected in DNA extracts by polymerase chain reaction assay separately. To determine the prevalence of biofilm formation, a modified Congo red agar and the microtiter plate method were used. Investigation of antibiotic resistance was performed using the disk diffusion method. Results: S. aureus (48.87%) was identified in 65 (48.87%) samples, of which 40 (61.53%) were confirmed to be MRSA. Among MRSA and methicillin-sensitive S. aureus (MSSA) isolates, 97.5% and 60% produced biofilm, respectively. Resistance of MRSA isolates to amikacin, ceftriaxone, ciprofloxacin, erythromycin, gentamicin, mupirocin, rifampin, tetracycline, and tobramycin was 64.1%, 76.92%, 51.28%, 87.18%, 71.8%, 10.26%, 5.13%, 89.74%, and 61.54%, respectively. All MRSA and MSSA isolates were susceptible to fusidic acid, linezolid, teicoplanin, tigecycline, and vancomycin. Conclusions: The high prevalence of biofilm-producing, drug-resistant S. aureus isolates in our study suggests that epidemiological studies on the characteristics of common strains found in burn centers and a definition of their antibiotic resistance pattern would be helpful for therapeutic decisions.

show abstract

“…As far as S. epidermidis is concerned, polysaccharide slime (also called biofilm) seems to be the most important factor by which it adheres to and colonizes artificial materials (31). As for S. aureus, it was well known, until now, for its ability to express molecules which recognize host matrix proteins (8,10,23,32). It has recently been shown that S. aureus as well as S. epidermidis is capable of forming slime (2,5,9,21,23).…”

mentioning

confidence: 99%

Presence of icaA and icaD Genes and Slime Production in a Collection of Staphylococcal Strains from Catheter-Associated Infections

2001

View full text Add to dashboard Cite

Both Staphylococcus epidermidis and Staphylococcus aureus are important causes of infections associated with catheters and other medical devices. It has recently been shown that not only S. epidermidis but also S. aureus can produce slime and carries the ica operon responsible for slime production. In the operon, coexpression of icaA and icaD is required for full slime synthesis. In this study, the presence of icaA and icaD was determined in a collection of 91 staphylococcal (68 S. epidermidis and 23 S. aureus) strains from intravenous catheterassociated infections, in 10 strains from the skin and mucosa of healthy volunteers, and in two reference strains by a PCR method. Slime-forming ability was tested on Congo red agar plates; 49% of S. epidermidis strains from catheters and, surprisingly, 61% of S. aureus strains were icaA and icaD positive and slime forming. All the saprophytic strains turned out to be negative for both icaA and icaD and also non-slime forming. Two S. aureus and one S. epidermidis strain from catheters, detected as icaA and icaD positive by PCR analysis and as slime forming (black colonies) at 24 h on Congo red agar, at 48 h exhibited tiny red spikes at the center of black colonies. The onset of these variants could not be ascribed to a mutagenic potential of Congo red, which, in the Ames test, was devoid of mutagenicity. PCR analysis showed that these red variants were negative for both icaA and icaD and even lacking the entire icaADBC operon. The data reported indicate an important role of ica genes as a virulence marker in staphylococcal infections from intravenous catheters.Staphylococcus epidermidis is a saprophyte which is part of the normal mucosa and skin microflora. In recent years, however, S. epidermidis emerged, together with Staphylococcus aureus, as a frequent etiologic agent of infections associated with catheters and other indwelling medical devices. As they possess little intrinsic pathogenic power, staphylococci are usually regarded as opportunistic agents (16,18). Over the last few years, several studies have been done to elucidate the structures and pathogenetic mechanisms by which staphylococci are able to cause severe and irreducible infections associated with biomaterials (4, 13, 22). As far as S. epidermidis is concerned, polysaccharide slime (also called biofilm) seems to be the most important factor by which it adheres to and colonizes artificial materials (31). As for S. aureus, it was well known, until now, for its ability to express molecules which recognize host matrix proteins (8,10,23,32). It has recently been shown that S. aureus as well as S. epidermidis is capable of forming slime (2, 5, 9, 21, 23).Recently, the genetic control of slime production has begun to be elucidated (17), first in S. epidermidis and then in S. aureus (9, 21). Synthesis of the capsular polysaccharide is mediated by the ica operon. Upon activation of this operon, a polysaccharide intercellular adhesin is synthesized. This supports cell-to-cell bacterial contacts by means of a multila...

show abstract

Role of Host and Bacterial Factors in Modulating Staphylococcal Adhesion to Implanted Polymer Surfaces

Cited by 51 publications

References 42 publications

Fibronectin Is More Active than Fibrin or Fibrinogen in Promoting Staphylococcus aureus Adherence to Inserted Intravascular Catheters

Fibronectin Is More Active than Fibrin or Fibrinogen in Promoting Staphylococcus aureus Adherence to Inserted Intravascular Catheters

Biofilm formation and antimicrobial resistance in methicillin-resistant Staphylococcus aureus isolated from burn patients, Iran

Presence of icaA and icaD Genes and Slime Production in a Collection of Staphylococcal Strains from Catheter-Associated Infections

Contact Info

Product

Resources

About