Abstract:Candida albicans mutants deficient in homologous recombination (HR) are extremely sensitive to the alkylating agent methyl-methane-sulfonate (MMS). Here, we have investigated the role of HR genes in the protection and repair of C. albicans chromosomes by taking advantage of the heat-labile property (55 °C) of MMS-induced base damage. Acute MMS treatments of cycling cells caused chromosome fragmentation in vitro (55 °C) due to the generation of heat-dependent breaks (HDBs), but not in vivo (30 °C). Following re… Show more
“…Here, three different aspects of Candida infections are examined: the maintenance of chromosomal integrity; biofilm formation as a form of survival; and the establishment of new models of infection as an alternative to mice. Ciudad et al [ 2 ] address the problem of repairing the alkylation base damage in the genome of C. albicans . After analyzing the response of three homologous recombination (HR) mutants to chromosomal damage caused by methyl methanesulfonate (MMS), these authors propose that repair takes place through a mechanism (possibly base excision repair) that does not involve homologous recombination.…”
“…Here, three different aspects of Candida infections are examined: the maintenance of chromosomal integrity; biofilm formation as a form of survival; and the establishment of new models of infection as an alternative to mice. Ciudad et al [ 2 ] address the problem of repairing the alkylation base damage in the genome of C. albicans . After analyzing the response of three homologous recombination (HR) mutants to chromosomal damage caused by methyl methanesulfonate (MMS), these authors propose that repair takes place through a mechanism (possibly base excision repair) that does not involve homologous recombination.…”
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