Role of HIF-1α in Cold Ischemia Injury of Rat Donor Heart Via the miR-21/PDCD4 Pathway

Zhu, Liang; Mu, Jingzhou; Wu, Yunhong; Feng, Shanshan; Qu, Chang; Cao, Tingting; Jing, Tangtang; Xie, Yan; Wen, Lianpu; Fan, Zida; Jia, Jie; Ma, Tonghui

doi:10.1016/j.transproceed.2019.11.001

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…Akt/protein kinase B (PKB) is a serine/threonine kinase that can be involved in tubular apoptosis and inflammatory response during renal ischemia-reperfusion injury ( 46 , 47 ). The transcription factor hypoxia-inducible factor-1α (HIF-1α) ( 48 ) has been associated to glomerulosclerosis and renal interstitial fibrosis ( 49 ). NCOAs include coactivators and corepressors and significantly boost nuclear receptor transcriptional activity.…”

Section: Discussionmentioning

confidence: 99%

The effect of Astragali Radix-Radix Angelica Sinensis on acute kidney injury: a network pharmacology and molecular docking study

Wang,

Zhen,

Lippi

et al. 2024

Transl Androl Urol

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Background Acute kidney injury (AKI) is a devastating clinical syndrome with high mortality rate attributed to lack of effective treatment. The herbal pair of Astragali Radix (AR) and Radix Angelica Sinensis (RAS) is a commonly prescribed herbal formula or is added to other traditional Chinese medicine (TCM) prescriptions for the treatment of kidney diseases. AR-RAS has certain protective effects on AKI in experiments, but the relevant mechanisms have yet to be clear. So this study aims to explore the mechanism of action of AR-RAS in AKI by combining network pharmacology and molecular docking methods. Methods In Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), the major AR-RAS chemical components and associated action targets were found and screened. The DrugBank and GeneCards databases were used to find AKI-related targets. The targets that are in close relationship with AKI were obtained from Therapeutic Target database (TTD), Online Mendelian Inheritance in Man (OMIM), and PharmGKB databases. The “herb-active ingredient-target” network was drawn by Cytoscape 3.8.0 software. The Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database was used to build the protein-protein interaction network. Bioconductor/R was used to examine Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. AR-RAS components and critical targets were docked using the AutoDock Vina program. Results A compound-target network, built by screening and analyzing the results, allowed to identify 19 active components and 101 possible therapeutic targets for AKI. The main ingredients were quercetin, kaempferol, 7-o-methylisocronulatol, formononetin and isorhamnetin. The key targets included AKT serine/threonine kinase 1 (AKT1), nuclear receptor coactivator 1 (NCOA1), JUN, estrogen receptor alpha (ESR1) and mitogen-activated protein kinase 8 (MAPK8). These molecules are targeted by pathways such as the calcium signaling route, the tumor necrosis factor (TNF) signaling pathway and the interleukin-17 (IL-17) signaling pathway, as well as the development of T helper 17 cells. Molecular docking demonstrated that AR-active RAS components exhibited strong binding activities to probable targets of AKI. Conclusions We described here the potential active ingredients, possible targets responsible for the efficacy of AR-RAS in AKI treatment, providing a theoretical basis for further research.

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Section: Discussionmentioning

confidence: 99%

The effect of Astragali Radix-Radix Angelica Sinensis on acute kidney injury: a network pharmacology and molecular docking study

Wang,

Zhen,

Lippi

et al. 2024

Transl Androl Urol

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“…Through the miR-21/PDCD4 pathway, HIF-1a can work on the myocardial ischemia injury in rats [77]. The IL-6/stat3 pathway leads to metastasis and chemotherapy resistance of hepatocellular carcinoma after interventional therapy through the HIF-1a/SNAI1 axis [78]. MALAT1 affects hypoxia-induced vascular endothelial cell damage and autophagy by regulating the miR-19b-3p/HIF-1a axis [79].…”

Section: Other Related Signalling Pathwaysmentioning

confidence: 99%

An overview of biological research on hypoxia-inducible factors (HIFs)

Liu

Fan

et al. 2020

Endokrynologia Polska

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“…Hypoxia-inducible factor-1 α (HIF-1 α ) is a transcription factor that plays a major role under hypoxic conditions. Various studies reported that the increased expression of HIF-1 α was associated with cancer risk and cancer prognosis [ 9 – 12 ]. However, currently, the effects of HIF and vascular endothelial growth factor (VEGF) are not well known.…”

Section: Introductionmentioning

confidence: 99%

Association of Nuclear Receptor Coactivators with Hypoxia-Inducible Factor-1α in the Serum of Patients with Chronic Kidney Disease

Zhou

Lin

et al. 2020

BioMed Research International

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Nuclear receptor coactivators (NCOAs), consisting of coactivators and corepressors, dramatically enhance the transcriptional activity of nuclear receptors. Hypoxia-inducible factor-1α (HIF-1α) is a transcription factor that plays a major role under hypoxic conditions. This study was performed with the focus on the association of NCOAs with HIF-1α in the serum of chronic kidney disease (CKD) patients. Sixty patients with stage 5 CKD and 30 healthy controls from The Second Affiliated Hospital of Shantou University Medical College, between March 21, 2019, and October 30, 2019, were recruited in this prospective cohort study. We analyzed the serum levels of NCOAs (NCOA1, NCOA2, and NCOA3), HIF-1α, vascular endothelial growth factor (VEGF), etc. and assessed whether there was any relationship between these parameters and CKD disease. We found that circulating NCOA1 was positively associated with circulating NCOA2, NCOA3, and HIF-1α. A positive correlation was also observed between NCOA2 and NCOA1, NCOA3, HIF-1α, and VEGF. Furthermore, statistically significant correlations between NCOA3 and NCOA1, NCOA2, and HIF-1α were observed. The serum levels of VEGF in the CKD group were higher than those of the healthy control group. Circulating NCOA1 and circulating NCOA2 were negatively associated with procalcitonin. In conclusion, there was an association between circulating NCOA1, NCOA2, NCOA3, and circulating HIF-1α, and circulating VEGF was a risk factor for CKD disease. However, more studies should be performed to confirm this hypothesis.

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Role of HIF-1α in Cold Ischemia Injury of Rat Donor Heart Via the miR-21/PDCD4 Pathway

Cited by 4 publications

References 22 publications

The effect of Astragali Radix-Radix Angelica Sinensis on acute kidney injury: a network pharmacology and molecular docking study

The effect of Astragali Radix-Radix Angelica Sinensis on acute kidney injury: a network pharmacology and molecular docking study

An overview of biological research on hypoxia-inducible factors (HIFs)

Association of Nuclear Receptor Coactivators with Hypoxia-Inducible Factor-1α in the Serum of Patients with Chronic Kidney Disease

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