Role of hageman factor in the initiation of clotting by glass

Ratnoff, Oscar D.; Rosenblum, Jerold M.

doi:10.1016/0002-9343(58)90023-8

Cited by 174 publications

(38 citation statements)

References 9 publications

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“…In the presence of Celite, collagen produced little or no further acceleration of clotting. These results suggest that collagen affects the same stages of coagulation as Celite which, like kaolin and glass, has been shown to activate factors XII and XI (9,(22)(23)(24)(25).…”

Section: Resultsmentioning

confidence: 82%

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Activation of hageman factor by collagen

Wilner¹,

Nossel²,

Leroy³

1968

J. Clin. Invest.

204

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A B S T R A C T Purified acid-soluble and insoluble human collagen accelerated the clotting of plateletpoor plasma in silicone-treated tubes. The clotpromoting effect did not appear to be due to thromboplastic activity since the collagen preparations did not activate factor X in the presence of factor VII and calcium. Instead, collagen appeared to accelerate clotting by activating Hageman factor (factor XII) on the basis of the following findings: collagen increased the clot-promoting activity of partially purified Hageman factor but exerted no further effect in the presence of kaolin, a known activator of Hageman factor; clot-promoting eluates were obtained from collagen exposed to normal, hemophilic, or PTC-deficient plasma but not from collagen exposed to Hageman or PTA-deficient plasma. The collagen molecule itself appeared to be required for the clot-promoting activity since digestion with collagenase or thermal denaturation at pH 2.5 (about 350C) resulted in very marked reduction in clot-promoting activity. Since thermal denaturation is associated with transformation of collagen structure from triple helical to random coil form, it is suggested that the native form of collagen is essential for the ability to activate Hageman factor.Blockage of the free amino groups by treatment with nitrous acid or dinitrofluorobenzene only slightly reduced the clot-promoting activity of collagen. In contrast, since addition of cationic proteins to collagen markedly reduced pro-coagulant activity it is suggested that negatively charged sites on the collagen molecule are critical for Hageman factor activation. This suggestion is supported by

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Section: Resultsmentioning

confidence: 82%

“…Celite eluates replace factor XII and XI activity to a much greater extent than collagen eluates do but are free of factor IX activity (Table V). The clot-promoting activity of a purified Hageman factor preparation was markedly increased by kaolin, a substance known to activate Hageman factor (22)(23)(24)(25), as well as by collagen (Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

Activation of hageman factor by collagen

Wilner¹,

Nossel²,

Leroy³

1968

J. Clin. Invest.

204

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“…The distribution of glass factor activity coincided with Hageman factor, suggesting that these may be identical. The identification of glass factor with Hageman factor has been previously suggested by Ratnoff and Rosenblum (17). It was possible to assay for PTA activity in only one run.…”

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confidence: 88%

Application of Continuous Flow Electrophoresis to the Study of the Blood Coagulation Proteins and the Fibrinolytic Enzyme System. I. Normal Human Materials1

Lewis¹,

Walters²,

Didisheim³

et al. 1958

J. Clin. Invest.

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Continuous flow filter paper electrophoresis, as developed by Svensson and Brattsen (1), Grassmann and Hannig (2-4) and Durrum (5), offers a method of plasma fractionation in which protein denaturation appears minimal. This study involves the testing of such fractions for biological activity in various coagulation systems. With the exception of fibrinogen, the coagulation proteins are present in plasma in minute amounts which, at the present time, can be assayed only by their activities in specific clotting systems. Many of these coagulation factors are readily denatured; thus, rigid control with particular attention to temperature is necessary to obtain good recovery of biological activity.Previous electrophoretic studies concerning distribution of plasma coagulation proteins have employed paper electrophoresis with elutions from cut strips. Owen and McKenzie (6) found prothrombin to coincide with a2 globulin, proconvertin (stable conversion factor) with ft globulin, proaccelerin (labile conversion factor) in a variable area between y and f8 peaks, antithrombin between /3 and a2, and fibrinogen remaining at the point of application. Using the same technique, Frick and Hagen (7) found Hageman factor activity in a fraction located between ft and -y globulin. On the other hand, the electrophoretic mobility of purified prothrombin was described by Seegers, McClaughry, and Fahey (8) as approximately equivalent to that of a, globulin. The

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“…Effect on Partial Thromboplastin Time Blood remains fluid within the body but quickly clots when placed in contact with a negatively charged surface, such as glass, quartz or kaolin (6). The series of reactions, leading to the clotting of blood, was called the cascade of coagulation by MacFarlane (7) and can be described as in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

Asbestos Fibers, Plasma and Inflammation

Hamilton¹

1983

Environmental Health Perspectives

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Fibrin clots have been detected at sites of inflammation, and kinins have been implicated as mediators of the vascular phenomena of acute inflammation, systemic shock, and disseminated intravascular coagulation. It is now reported that both negatively and positively charged asbestos fibers shorten the partial thromboplastin time of human plasma, indicating coagulation of the plasma. A sample containing short (<5 Mm in length) chrysotile fibers is ineffective. Only the negatively charged amphiboles (crocidolite and amosite) are able to activate factor XII (Hageman factor). This particular effect of the amphiboles is enhanced by high molecular weight kininogen and leads to kinin formation.

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Role of hageman factor in the initiation of clotting by glass

Cited by 174 publications

References 9 publications

Activation of hageman factor by collagen

Activation of hageman factor by collagen

Application of Continuous Flow Electrophoresis to the Study of the Blood Coagulation Proteins and the Fibrinolytic Enzyme System. I. Normal Human Materials1

Asbestos Fibers, Plasma and Inflammation

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