Role of granulocyte colony-stimulating factor in infection and inflammation

Hartung, Thomas; Aulock, Sonja von; Wendel, Albrecht

doi:10.1007/s004300050075

Cited by 25 publications

(23 citation statements)

References 26 publications

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“…Fibroblasts, lymphocytes, endothelial cells, and neutrophils can release substantial amounts of G-CSF (40). Monocytes and macrophages are nevertheless considered the main cellular sources upon activation with cytokines or lipopolysaccharide (40).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Monocytes and macrophages are nevertheless considered the main cellular sources upon activation with cytokines or lipopolysaccharide (40). G-CSF is a hematopoietic growth factor for neutrophils and monocytes (40). It is basically a proinflammatory mediator that primes neutrophils for chemotaxis and phagocytosis, thereby leading to the release of reactive oxygen species and lysosomal enzymes (40).…”

Section: Discussionmentioning

confidence: 99%

“…G-CSF is a hematopoietic growth factor for neutrophils and monocytes (40). It is basically a proinflammatory mediator that primes neutrophils for chemotaxis and phagocytosis, thereby leading to the release of reactive oxygen species and lysosomal enzymes (40). Increased circulating levels of elastase (12,14,15) and alpha-1-antitrypsin (15) have been found in D ϩ HUS, as well as in HC (41).…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Circulating Granulocyte Colony-Stimulating Factor, C-X-C, and C-C Chemokines in Children with Escherichia Coli O157:H7 Associated Hemolytic Uremic Syndrome

et al. 2002

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Circulating Granulocyte Colony-Stimulating Factor, C-X-C, and C-C Chemokines in Children with Escherichia Coli O157:H7 Associated Hemolytic Uremic Syndrome

et al. 2002

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“…For several of these genes the findings support our previously described model of the intestinal epithelium as a sensor for infection with invasive enteric pathogens that can provide proinflammatory and chemoattractant signals to cells in the underlying lamina propria (3). For example, G-CSF is a growth and activation factor for neutrophils and their precursors (22) and could thereby complement the functions of epithelial cell-derived neutrophil chemoattractants such as IL-8 (23). Similarly, MIP-2␣ (also termed GRO␤), whose epithelial up-regulation after Salmonella infection was identified here, is closely related in structure and function to the previously studied GRO family members, GRO␣ and GRO␥, all of which have chemotactic and activating functions for neutrophils (6,23).…”

Section: Importance Of Nf-b In Regulating Increased Mrna Expression Imentioning

confidence: 99%

Analysis by High Density cDNA Arrays of Altered Gene Expression in Human Intestinal Epithelial Cells in Response to Infection with the Invasive Enteric BacteriaSalmonella

Eckmann

Smith

Housley

et al. 2000

Journal of Biological Chemistry

171

108

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Many clinically important enteric pathogens initiate disease by invading and passing through the intestinal epithelium, a process accompanied by increased epithelial expression of proinflammatory cytokines. To further define the role intestinal epithelial cells play in initiating and modulating the host response to infection with invasive bacteria, hybrid selection on high density cDNA arrays was used to characterize the mRNA expression profile of ϳ4,300 genes in human intestinal epithelial cells after infection with the prototypic invasive bacteria, Salmonella. Selected findings were further evaluated by reverse transcription-polymerase chain reaction, Northern blot analysis, and protein assays. Epithelial infection with Salmonella significantly up-regulated mRNA expression of a relatively small fraction of all genes tested. Of these, several cytokines (granulocyte colony-stimulating factor, inhibin A, Epstein-Barr virusinduced gene 3, interleukin-8, macrophage inflammatory protein-2␣), kinases (TKT, Eck, HEK), transcription factors (interferon regulatory factor-1), and HLA class I were the most prominent. Furthermore, the transcription factor NF-B is shown to be important for inducible mRNA expression for a broad group of genes tested. These findings expand the repertoire of known epithelial cell responses to infection with an invasive enteric pathogen. The results also show that evaluation of mRNA expression profiles by cDNA array analysis is a powerful approach to characterizing and understanding host-pathogen interactions.

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A New Strategy for the Synthesis of Dinucleotides Loaded with Glycosylated Amino Acids—Investigations on in vitro Non‐natural Amino Acid Mutagenesis for Glycoprotein Synthesis

et al. 2005

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The in vitro non-natural amino acid mutagenesis method provides the opportunity to introduce non-natural amino acids site-specifically into proteins. To this end, a chemically synthesised aminoacylated dinucleotide is enzymatically ligated to a truncated suppressor transfer RNA. The loaded suppressor tRNA is then used in translation reactions to read an internal stop codon. Here we report an advanced and general strategy for the synthesis of the aminoacyl dinucleotide. The protecting group pattern developed for the dinucleotide facilitates highly efficient aminoacylation, followed by one-step global deprotection. The strategy was applied to the synthesis of dinucleotides loaded with 2-acetamido-2-deoxy-glycosylated amino acids, including N- and O-beta-glycosides and O- and C-alpha-glycosides of amino acids, thus enabling the extension of in vitro non-natural amino acid mutagenesis towards the synthesis of natural glycoproteins of high biological interest. We demonstrate the incorporation of the glycosylamino acids--although with low suppression efficiency--into the human interleukin granulocyte-colony stimulating factor (hG-CSF), as verified by the ELISA technique.

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Role of granulocyte colony-stimulating factor in infection and inflammation

Cited by 25 publications

References 26 publications

Circulating Granulocyte Colony-Stimulating Factor, C-X-C, and C-C Chemokines in Children with Escherichia Coli O157:H7 Associated Hemolytic Uremic Syndrome

Circulating Granulocyte Colony-Stimulating Factor, C-X-C, and C-C Chemokines in Children with Escherichia Coli O157:H7 Associated Hemolytic Uremic Syndrome

Analysis by High Density cDNA Arrays of Altered Gene Expression in Human Intestinal Epithelial Cells in Response to Infection with the Invasive Enteric BacteriaSalmonella

A New Strategy for the Synthesis of Dinucleotides Loaded with Glycosylated Amino Acids—Investigations on in vitro Non‐natural Amino Acid Mutagenesis for Glycoprotein Synthesis

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