Role of glycoprotein 130 and c-Kit signaling in proliferation and differentiation of human hematopoietic progenitor cells

Nakahata, Tatsutoshi; Sui, Xingwei; Tanaka, Ryuhei; Tajima, S; Muraoka, Kaori; Ebihara, Yasuhiro; Tsuji, Kohichiro

doi:10.1007/s002800051041

Cited by 12 publications

(7 citation statements)

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“…Studies in vitro also demonstrated that IL-6 was a permissive factor for monocytic colony formation by human HPC and its paracrine production by bone marrow monocytes was responsible for this process [23]. Furthermore, recent studies have shown that both proliferation and differentiation of human HPC could be induced by IL-6-IL-6sR complex, through gp 130 signalling on stem cells, in the presence of SCF [24].…”

Section: Discussionmentioning

confidence: 97%

Surgical Trauma Evokes a Rise in the Frequency of Hematopoietic Progenitor Cells and Cytokine Levels in Blood Circulation

Grzelak

Olszewski²,

Zaleska³

et al. 1998

Eur Surg Res

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Alterations in the mononuclear cell populations in the blood circulation are among the most characteristic changes after surgical trauma. They reflect changes in the hematopoietic compartment which develop following surgery. The process of mobilization and differentiation of the hematopoietic population is regulated by cytokines known as growth factors for stem and progenitor cells (SCF, IL-1, IL-3, IL-6, IL-11, TNF, CSFs). Our question was whether operative trauma resulted in the release of the hematopoietic progenitor cells to the blood circulation and an increase in the blood level of cytokines participating in hematopoiesis. The studies were carried out in patients with chronic cholelithiasis, undergoing elective open cholecystectomy under general anesthesia. An increase in the frequency of circulating CD34+ hematopoietic progenitor cells was seen between days 3 and 7 after surgery. Moreover, a significant increase in the percentage of immature cells of myeloid lineage (CD13+, CD14+, CD33+) was seen on the 1st and 3rd postoperative days. This could be the result of an expansion of the total bone marrow cell number after surgery and a subsequent release of these cells into the blood circulation. The changes in blood cell populations were accompanied by an increase in IL-6 on days 1, 3, and 7 following surgery, in IL-6sR on days 10 and 14 and in IL-8 on days 1 and 3. No significant changes in IL-1α, IL-1β, IL-3 and IL-11 were noted. A small rise in GM-CSF was noted in few patients on the 3rd and 7th postoperative days. It is known that IL6 is involved in hematopoiesis, that the IL-6-IL-6sR complex may induce both proliferation and differentiation of hematopoietic progenitor cells and that IL-8 possesses progenitor cell mobilization properties. The appearance of hematopoietic progenitor cells in the blood following surgery may represent a process for the expansion of the immune cell pool after trauma and maintaining of the reserves at a certain level.

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Section: Discussionmentioning

confidence: 97%

Surgical Trauma Evokes a Rise in the Frequency of Hematopoietic Progenitor Cells and Cytokine Levels in Blood Circulation

Grzelak

Olszewski²,

Zaleska³

et al. 1998

Eur Surg Res

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“…The common transducing signal chain gp130, for all receptors of the IL-6 cytokine family including IL-6, IL-11, oncostatin M, leukemia inhibitory factor, ciliary neurotrophic factor, and cardiotrophin, is homodimerized by both IL-6 and IL-11 in murine bone marrow stroma [24]. gp130 is expressed in most primitive human hemopoietic CD34 + cells [25], and its signaling was shown in numerous studies to synergize with other hemopoietic cytokines to stimulate trilineage human hemopoiesis and expand primitive human hemopoietic stem cells [26][27][28][29][30][31][32]. Also, the addition of anti-gp130 monoclonal antibodies completely abrogated the synergistic action [26][27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

“…gp130 is expressed in most primitive human hemopoietic CD34 + cells [25], and its signaling was shown in numerous studies to synergize with other hemopoietic cytokines to stimulate trilineage human hemopoiesis and expand primitive human hemopoietic stem cells [26][27][28][29][30][31][32]. Also, the addition of anti-gp130 monoclonal antibodies completely abrogated the synergistic action [26][27][28][29][30]. Moreover, the genetic insertion of gp130 into primitive hemopoietic stem cells from murine bone marrow has been shown to expand the proliferation of these cells in the presence of stem cell factor [33].…”

Section: Discussionmentioning

confidence: 99%

Mechanisms Mediating the Inhibitory Effect of All‐Trans Retinoic Acid on Primitive Hematopoietic Stem Cells in Human Long‐Term Bone Marrow Culture

et al. 2000

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All-trans retinoic acid (RA) has generally been found to stimulate late committed (colony-forming unitgranulocyte, macrophage [CFU-GM]) and inhibit early (CFU-Blast) normal human myeloid progenitor cells. The present study provides the first evidence that the pharmacological concentration of 1 µM RA, exerts an inhibitory effect on the proliferation of functional human primitive hemopoietic stem cells (cobblestone area-forming cell [CFAC]) in long-term bone marrow cultures. Treatment of four-week confluent bone marrow culture with 1 µM RA for five days significantly reduced week 4 CAFC from 88 ± 10 in control cultures to only 52 ± 12 per 10 5 cells, p < 0.01. Quantitative enzyme-linked immunosorbent assay measurement of interleukin 6 (IL-6) and IL-11 produced from the fourweek bone marrow stroma culture revealed only a slight and moderate increase of IL-6 and IL-11 production after treatment with RA. On the other hand, treatment with RA profoundly increased the soluble receptor gp130 released from the four-week bone marrow stroma by 7.5-fold from only 145 ± 2.1 pg per ml in control cultures to 1,069.9 ± 3.8 pg per ml in RA-treated cultures. A similar marked increase in the soluble adhesion molecules ICAM-1, and to a lesser extent VCAM-1, released from the four-week bone marrow stroma was observed after RA treatment.IL-6 has been implicated in the inhibitory effect of RA in several human hemopoietic and nonhemopoietic cells. The common transducing signal chain gp130, for all receptors of the IL-6 cytokine family, is expressed in most primitive human hemopoietic CD34 + cells and its signaling was shown to synergize with other hemopoietic cytokines to expand primitive human hemopoietic stem cells. Recently, soluble gp130 was shown to be a natural potent antagonist of the human IL-6 cytokine family by binding the ligand and thereby reducing its bioavailability.The profound and rapid 7.5-fold increase in the natural antagonist of human IL-6 cytokine family after RA treatment could abrogate the gp130 signaling required for proliferation and/or expansion of human primitive hemopoietic stem cells and lead to the observed inhibitory effect of RA on CAFC. Both adhesion molecules VCAM-1 and ICAM-1 mediate human hemopoietic stem cell adhesion to marrow stroma. The present significant increase in the soluble form of these adhesion molecules after RA treatment could exert a significant antagonist effect on their function and hence may impair CAFC adhesion to marrow stroma.In conclusion, the RA inhibitory effect on the proliferation of primitive human hemopoietic stem cells could be mediated through: A) an impaired hemopoietic stem cell adhesion due to the significant increase in soluble adhesion molecules released from the marrow stroma after RA treatment, and B) a significantly reduced gp130 signaling that is necessary for stem cell proliferation due to the natural antagonistic effect of the profoundly increased level of soluble gp130 released from the marrow stroma after treatment with RA.

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“…In this study, we proposed a new biointerface to capture cells. This biointerface have affinity with glycoproteins, which play the important roles in cell adhesion, proliferation, and differentiation as the antenna molcules of cell membrane [6,7]. First, we designed and synthesized a phospholipid polymer (PMBV) composed of 2-methacryloyloxyethyl phosphorylcholine (MPC), n-butyl methacrylate (BMA), and 4-vinylphenylboronic acid units (VPBA).…”

Section: Introductionmentioning

confidence: 99%

Glycoprotein recognition for cell capturing on artificial lectin surface

Saito

Konno

Ikake

et al. 2010

Trans. Mat. Res. Soc. Japan

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We prepared a new biointerface for obtaining specific recognition of glycoproteins having polysaccharide chains to capture corresponding cells by a phospholipid polymer having phenylboronic acid moiety (PMBV), so-called artificial lectin. The PMBV was coated on the surfaces of Au substrate and tissue culture polystyrene. The affinity of the PMBV toward the glycoproteins and its effects on cell adhesion were evaluated. X-ray photoelectron spectroscopic analysis revealed that the PMBV existed on both substrates. Although adsorption of protein without polysaccharide chain was suppressed, glycoprotein as fibronectin (FN) adsorbed effectively, which was confirmed by surface plasmon resonance. Kinetic parameters calculated by SPR measurements showed high affinity between PMBV and FN, 1.4×10 5 (M -1 ). Cell adhesion and growth behavior on PMBV was observed using L-929 fibroblast cells. It was demonstrated that cells proliferated well on PMBV surface and maintained their sphere morphology. Also, the activity of cells was kept in higher level on the PMBV surface compared with conventional surface.

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Role of glycoprotein 130 and c-Kit signaling in proliferation and differentiation of human hematopoietic progenitor cells

Cited by 12 publications

References 0 publications

Surgical Trauma Evokes a Rise in the Frequency of Hematopoietic Progenitor Cells and Cytokine Levels in Blood Circulation

Surgical Trauma Evokes a Rise in the Frequency of Hematopoietic Progenitor Cells and Cytokine Levels in Blood Circulation

Mechanisms Mediating the Inhibitory Effect of All‐Trans Retinoic Acid on Primitive Hematopoietic Stem Cells in Human Long‐Term Bone Marrow Culture

Glycoprotein recognition for cell capturing on artificial lectin surface

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