Role of Global Longitude Strain related miRNAs as potential prognostic indicators in myocardial infarction

Abstract

BACKGROUND Real-time three-dimensional spot tracking echocardiography (RT3D-STE) can be used to assess the prognosis of myocardial infarction (MI). Analyzing the correlation between miRNAs and RT3D-STE, especially miRNAs and Global Longitude Strain (GLS) and Global Circumferential Strain (GCS), can provide new ideas for discovering and evaluating the role of miRNAs in assessing myocardial function and prognosis of MI. METHODS The present study collected forty-nine (49) plasma samples (15 normal samples and 34 MI samples; 22 before and 12 after intervention therapy) and employed high-throughput sequencing technology and real-time quantitative polymerase chain reaction (qRT–PCR) to identify the differentially expressed miRNAs. The Pearson correlation coefficient was used to measure the strength of a linear association between differentially expressed miRNAs and strain parameters of RT3D-STE. RESULTS One hundred forty-one (141) differentially expressed plasma exosome miRNAs were identified in STEMI compared with NSTEMI. The target genes of these ten miRNAs were analyzed for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and they were found to be involved mainly in cellular metabolism processes, HIF-1 signaling pathway, and the FoxO signaling pathway. Moreover, three of these differentially expressed miRNAs (hsa-miR-4798-3p, hsa-miR-371a-3p and hsa-miR-4735-5p) had Pearson correlations greater than 0.5 based on GLS. CONCLUSION Differentially expressed miRNAs obtained before and after surgical therapy of MI may play important roles in the occurrence and development of MI. hsa-miR-4798-3p, hsa-miR-371a-3p, and hsa-miR-4735-5p may be used as potential prognostic indicators of MI.