Role of genetic resources and molecular markers in<i>Mucuna pruriens</i>(L.) DC improvement

Sathyanarayana, N.; Mahesh, S.; Leelambika, M.; Jaheer, Mahammad; Chopra, Ratan; Rashmi, K.

doi:10.1017/s1479262116000071

Cited by 10 publications

(4 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a predominantly self-pollinating species like M. pruriens, we expect lower levels of genetic diversity. But higher values recorded here and in some earlier studies (summarized inSathyanarayana et al 2016) categorically points to presence of out-crossing in this species as observed initially byPadmesh et al (2006). This is partly supported by a high average gene ow among the population groups (Nm = 3.03).…”

supporting

confidence: 79%

Association mapping of important agronomic traits in Mucuna pruriens (L.) DC.

Lepcha

Mahesh²,

Leelambika³

et al. 2023

Preprint

View full text Add to dashboard Cite

The Mucuna pruriens (L.) DC. can meet three burgeoning needs of tropical agriculture: low-cost protein source, high-value medicinal plant, and an excellent green manure cover crop. But like other underutilized crops, it lacks modern breeding resources. Identifying marker-trait associations (MTAs) can prompt marker-assisted breeding paving a way for development of the improved varieties. Recent studies have demonstrated feasibility of identifying MTAs using a small number of accessions (<100) triggering the hope for resource poor crops. Taking cues from there, we characterized a panel of 70 M. pruriens accessions across two consecutive years and performed association analysis for 16 phenotypic traits using 66 genic-microsatellite markers. The results showed significant phenotypic (P < 0.05) and genetic diversity (Shannon’s information index, I = 0.62) in our germplasm collection. Many of the tested traits were highly heritable (42.86% to 99.93%). A total of 15 MTAs were detected at an adjusted significance level of P < 5.55 × 10-3 for nine economic traits such as – seed length, seed thickness, seed width, hundred seed weight, seed yield per plant, inflorescence length, flower buds per inflorescence, flower length, and petiole length with phenotypic variance explained (PVE) ranging from 14.72 to 31.12%. Their annotation using the Arabidopsis genome database affirmed reliability of our MTAs. This is the first report on association mapping in M. pruriens and results are expected to galvanize marker-assisted breeding as well as mining of candidate genes in this promising legume.

show abstract

supporting

confidence: 79%

Association mapping of important agronomic traits in Mucuna pruriens (L.) DC.

Lepcha

Mahesh²,

Leelambika³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Mucuna pruriens (L.) DC. (velvet bean) is reported to be native to China and East India [ 5 ] but has now attained a pantropical distribution with a major niche in the Indian sub-continent [ 6 ]. Like common bean and mung bean, velvet bean has a chromosome number of 2n = 2x = 22 [ 7 ], but with a much larger estimated genome size of between 1,281 to 1,361 Mbp/C (A.N.…”

Section: Introductionmentioning

confidence: 99%

“…With the advent of genomic tools that can aid in developing molecular markers, genetic maps etc., the genetic improvement of underutilized crops has been greatly facilitated, enabling the development of improved genotypes or varieties with enhanced trait values [ 21 , 22 ]. However, studies focusing on the development of genomic resources of M. pruriens are lacking, with only a few reports available on the use of molecular markers such as RAPDs and AFLPs (reviewed in [ 6 ]). In recent years, transcriptome sequencing has emerged as an efficient method to generate genomic-level data, large expressed sequence tag (EST) sequences, and molecular markers.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic resources for the medicinal legume Mucuna pruriens: de novo transcriptome assembly, annotation, identification and validation of EST-SSR markers

et al. 2017

Self Cite

View full text Add to dashboard Cite

BackgroundThe medicinal legume Mucuna pruriens (L.) DC. has attracted attention worldwide as a source of the anti-Parkinson’s drug L-Dopa. It is also a popular green manure cover crop that offers many agronomic benefits including high protein content, nitrogen fixation and soil nutrients. The plant currently lacks genomic resources and there is limited knowledge on gene expression, metabolic pathways, and genetics of secondary metabolite production. Here, we present transcriptomic resources for M. pruriens, including a de novo transcriptome assembly and annotation, as well as differential transcript expression analyses between root, leaf, and pod tissues. We also develop microsatellite markers and analyze genetic diversity and population structure within a set of Indian germplasm accessions.ResultsOne-hundred ninety-one million two hundred thirty-three thousand two hundred forty-two bp cleaned reads were assembled into 67,561 transcripts with mean length of 626 bp and N50 of 987 bp. Assembled sequences were annotated using BLASTX against public databases with over 80% of transcripts annotated. We identified 7,493 simple sequence repeat (SSR) motifs, including 787 polymorphic repeats between the parents of a mapping population. 134 SSRs from expressed sequenced tags (ESTs) were screened against 23 M. pruriens accessions from India, with 52 EST-SSRs retained after quality control. Population structure analysis using a Bayesian framework implemented in fastSTRUCTURE showed nearly similar groupings as with distance-based (neighbor-joining) and principal component analyses, with most of the accessions clustering per geographical origins. Pair-wise comparison of transcript expression in leaves, roots and pods identified 4,387 differentially expressed transcripts with the highest number occurring between roots and leaves. Differentially expressed transcripts were enriched with transcription factors and transcripts annotated as belonging to secondary metabolite pathways.ConclusionsThe M. pruriens transcriptomic resources generated in this study provide foundational resources for gene discovery and development of molecular markers. Polymorphic SSRs identified can be used for genetic diversity, marker-trait analyses, and development of functional markers for crop improvement. The results of differential expression studies can be used to investigate genes involved in L-Dopa synthesis and other key metabolic pathways in M. pruriens.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3780-9) contains supplementary material, which is available to authorized users.

show abstract

“…Also, it has been found to reactivate the antioxidant defence system, necessary for maintaining homeostasis within the body. For further details, see Sathyanarayana et al (2016).…”

Section: Mucuna Pruriens (L) DC Velvet Beanmentioning

confidence: 99%

Exotic ancient plant introductions: part of Indian ‘Ayurveda’ medicinal system

Singh

2016

Plant Genet. Resour.

View full text Add to dashboard Cite

India is home to some of the oldest civilizations, during which period the local communities domesticated indigenous plant species for food and agriculture and medicinal uses. In this process, they also bio-prospected and/or absorbed potentially valuable exotic plant species, making them integral part of Indian culture, including the traditional medicinal system, the Ayurveda. The present paper discusses the absorption of 26 plant species of exotic origin, before 8th century, as evidenced by archaeological sculptural or botanical remains and documentation of such plants in Sanskrit, the Vedic language. Occurrence and/or introduction of such plants at such distant places in ancient times is visualized as a result of geographical continental fragmentation followed by drift, natural or man-made transoceanic movement, and cultural and trade exchange of plant material over time and space.

show abstract

Role of genetic resources and molecular markers inMucuna pruriens(L.) DC improvement

Cited by 10 publications

References 72 publications

Association mapping of important agronomic traits in Mucuna pruriens (L.) DC.

Association mapping of important agronomic traits in Mucuna pruriens (L.) DC.

Transcriptomic resources for the medicinal legume Mucuna pruriens: de novo transcriptome assembly, annotation, identification and validation of EST-SSR markers

Exotic ancient plant introductions: part of Indian ‘Ayurveda’ medicinal system

Contact Info

Product

Resources

About