Role of galE on biofilm formation by Thermus spp.

Niou, Yi-Kai; Wu, Wan-Chen; Lin, Ling-Chun; Yu, Mei-Shiuan; Shu, Hung-Yu; Yang, Hsin-Chia; Lin, Guang-Huey

doi:10.1016/j.bbrc.2009.09.120

Cited by 33 publications

(23 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to Cava et al (71), the composition of polysaccharides detected in T. thermophilus cell wall is 74% reducing sugars. In addition, previous studies indicated that in T. thermophilus HB27, the overexpression of GalE, which participates in the Leloir pathway for galactose metabolism, leads to an increase in biofilm formation (14). In this study, these mutant strains also incorporated a higher proportion (ϳ75%) of galactose into the EPS on their cell wall (supplemental Table S7).…”

Section: Phosphorylated Pilf Interferes With Pilus Formation and Retamentioning

confidence: 50%

“…ity, T4P is known to involve in the formation of complex colonial structures in biofilms (60). In a previous study, we demonstrated the ability of T. thermophilus HB27 to form biofilms (14). Thus, in the present work we further investigated the effect of PilF phosphorylation on biofilm-formation ability, using crystal violet staining and scanning electron microscopy.…”

Section: List Of Identified Phosphoproteins and Phosphopeptides From mentioning

confidence: 99%

“…that developed on microtiter plates or glass slides were characterized as described previously (14). The inoculated broth cultures were grown to stationary phase in 5 ml of TM medium at 70°C and then used to prepare serial dilutions in fresh medium down to A 600 ϭ 0.006.…”

Section: Overexpression Of Recombinant Pilfs In T Thermophilus Hb27-mentioning

confidence: 99%

“…In previous studies, a novel exopolysaccharide, TA-1, was isolated from a T. aquaticus YT-1 biofilm, and both its primary structure and its immunological activity were determined (13). In addition, we showed that the overexpression of uridine diphosphate (UDP)-galactose-4Ј-epimerase (GalE), which catalyzes the reversible interconversion of UDP-galactose and UDP-glucose, in T. thermophilus HB27 increases biofilm production because of the enzyme's involvement in an important step of exopolysaccharide (EPS) 1 biosynthesis (14). The other mechanism that enables Thermus to thrive in extreme habitats is natural trans-formation (i.e.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Phosphoproteomic Analysis Reveals the Effects of PilF Phosphorylation on Type IV Pilus and Biofilm Formation in Thermus thermophilus HB27

Liao

Lin

et al. 2013

Molecular & Cellular Proteomics

Self Cite

View full text Add to dashboard Cite

Thermus thermophilus HB27 is an extremely thermophilic eubacteria with a high frequency of natural competence. This organism is therefore often used as a thermophilic model to investigate the molecular basis of type IV pilimediated functions, such as the uptake of free DNA, adhesion, twitching motility, and biofilm formation, in hot environments. In this study, the phosphoproteome of T. thermophilus HB27 was analyzed via a shotgun approach and high-accuracy mass spectrometry. Ninety-three unique phosphopeptides, including 67 in vivo phosphorylated sites on 53 phosphoproteins, were identified. The distribution of Ser/Thr/Tyr phosphorylation sites was 57%/36%/7%. The phosphoproteins were mostly involved in central metabolic pathways and protein/cell envelope biosynthesis. According to this analysis, the ATPase motor PilF, a type IV pili-related component, was first found to be phosphorylated on Thr-368 and Ser-372. Through the point mutation of PilF, mimic phosphorylated mutants T368D and S372E resulted in nonpiliated and nontwitching phenotypes, whereas nonphosphorylated mutants T368V and S372A displayed piliation and twitching motility. In addition, mimic phosphorylated mutants showed elevated biofilm-forming abilities with a higher initial attachment rate, caused by increasing exopolysaccharide production. In summary, the phosphorylation of PilF might regulate the pili and biofilm formation associated with exopolysaccharide production. Molecular & Cellular Proteomics

show abstract

Section: Phosphorylated Pilf Interferes With Pilus Formation and Retamentioning

confidence: 50%

Section: List Of Identified Phosphoproteins and Phosphopeptides From mentioning

confidence: 99%

Section: Overexpression Of Recombinant Pilfs In T Thermophilus Hb27-mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Phosphoproteomic Analysis Reveals the Effects of PilF Phosphorylation on Type IV Pilus and Biofilm Formation in Thermus thermophilus HB27

Liao

Lin

et al. 2013

Molecular & Cellular Proteomics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another identified protein named UDP-galactose 4-epimerase (GALE) was also down-reg- ulated in thiram treated chondrocytes. GALE protein is an enzyme responsible for the epimerization of UDP-glucose and UDP-N-acetyl glucosamine to UDP-galactose, and UDP-N-acetyl galactosamine, and vice versa (Niou et al, 2009;Bang et al, 2009). GALE plays crucial role in energy metabolism through production of UDP-glucose and UDP-N-acetyl glucosamine necessary for mucopolysaccharide, glycoprotein, glycolipid, and the proteoglycan biosynthesis (Sweeney et al, 1993;Lai et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Effect of thiram on avian growth plate chondrocytes in culture

Rasaputra

Liyanage²,

Lay³

et al. 2013

J. Toxicol. Sci.

View full text Add to dashboard Cite

-Thiram is a dithiocarbamate pesticide that causes tibial dyschondroplasia (TD), a growth plate defect, in poultry. Deaths of transitional zone chondrocytes appear to interrupt endochondral bone development leading to the broadening of growth plate. The mechanism of action of thiram on chondrocytes is not well understood. Since proteins play major roles in different aspects of cell's metabolism, growth, and survival, the objective of this study was to find whether thiram produces proteomic changes that could impair the development of chondrocytes. The chondrocytes, isolated from proximal tibial growth plates, were cultured with or without a sub-lethal concentration of thiram for 48 hr, and the cell proteins were extracted, and subjected to 2-D gel electrophoresis. The gel images were compared and statistically evaluated using Melanie software to identify differentially expressed protein spots. Of a total of 72 identifiable spots 3 were down-regulated and 2 up-regulated in thiram treated chondrocytes. In-gel trypsin digestion of the protein spots followed by their characterization by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry identified 25 spots comprising of 23 proteins. Two of 3 down-regulated proteins were identified as a heat shock protein 70 (HSP 70) and a GALE (UDP-galactose-4 epimerase) protein isoform I. The up-regulated proteins were Serpin H1, a protein involved in collagen metabolism and a redox sensor NmrA-like (NMRAL) family domain protein-1. Both GALE and NMRAL proteins are implicated in energy metabolism and redox regulation whereas the HSP 70 protects cells against stress, and implicated in chondrocyte hypertrophy, an important event in endochondral bone formation. The failure of chondrocyte protective mechanisms such as associated with protection against cellular stress and energy metabolism appear to be the likely cause for chondrocyte death induced by thiram.

show abstract

Determinants of the Nucleotide Specificity in the Carbohydrate Epimerase Family 1

Overtveldt

Costa

Gevaert

et al. 2020

Biotechnology Journal

View full text Add to dashboard Cite

In recent years, carbohydrate epimerases have attracted increasing attention as promising biocatalysts for the production of specialty sugars and derivatives. The vast majority of these enzymes are active on nucleotide‐activated sugars, rather than on their free counterparts. Although such epimerases are known to have a clear preference for a particular nucleotide (UDP, GDP, CDP, or ADP), very little is known about the determinants of the respective specificities. In this work, sequence motifs are identified that correlate with the different nucleotide specificities in one of the main epimerase superfamilies, carbohydrate epimerase 1 (CEP1). To confirm their relevance, GDP‐ and CDP‐specific residues are introduced into the UDP‐glucose 4‐epimerase from Thermus thermophilus, resulting in a 3‐fold and 13‐fold reduction in KM for GDP‐Glc and CDP‐Glc, respectively. Moreover, several variants are severely crippled in UDP‐Glc activity, which further underlines the crucial role of the identified positions. Hence, the analysis should prove to be valuable for the further exploration and application of epimerases involved in carbohydrate synthesis.

show abstract

Role of galE on biofilm formation by Thermus spp.

Cited by 33 publications

References 38 publications

Phosphoproteomic Analysis Reveals the Effects of PilF Phosphorylation on Type IV Pilus and Biofilm Formation in Thermus thermophilus HB27

Phosphoproteomic Analysis Reveals the Effects of PilF Phosphorylation on Type IV Pilus and Biofilm Formation in Thermus thermophilus HB27

Effect of thiram on avian growth plate chondrocytes in culture

Determinants of the Nucleotide Specificity in the Carbohydrate Epimerase Family 1

Contact Info

Product

Resources

About